Category: View from the Bench

July 29, 2022

28 April

Temperature & C. elegans Part Two: How does Temperature affect development?

By:

Christine Kim ,

Thomas Adams ,

April 28, 2022

14 April

The Benefits of Standardizing Zebrafish Diets

By:

Gianna DeMarco ,

Devon Scallion ,

April 14, 2022

24 March

How Do Knockins Work?

By:

Karl Stuntzner-Gibson ,

March 24, 2022

15 March

Zebrafish 101: A White Paper

By:

March 15, 2022

9 March

C. elegans 101: A White Paper

By:

March 9, 2022

24 February

Why is temperature important for C. elegans?

By:

February 24, 2022

3 February

Considerations When Adding New Zebrafish Racks

By:

Devon Scallion ,

Gianna DeMarco ,

February 3, 2022

27 January

Tips and Tricks for Successful Worm Picking

By:

Laura Taggart-Murphy ,

Christine Kim ,

Dan Richards ,

January 27, 2022

22 December

What is the new Split hygromycin method? How does it differ from MosSCI? How can we use it to help you?

By:

Marilee Hoyle ,

Anna Malinkevich, MS ,

December 22, 2021

21 July

Which Popular Live Food is Best for Your Zebrafish? A Head-to-Head Comparison of Rotifers and Artemia

By:

Madeleine Bunnenberg-Ross ,

July 21, 2021

19 July

Zebrafish Husbandry 101: Crossing Basics

By:

July 19, 2021

8 July

Zebrafish Husbandry 101: Embryo Injections

By:

July 8, 2021

30 June

Zebrafish Husbandry 101: Maintaining Water Quality

By:

June 30, 2021

28 June

Zebrafish Husbandry 101: Tracking Fish Health

By:

June 28, 2021

23 June

Zebrafish Husbandry 101: Live Food vs. Dry Food

By:

June 23, 2021

18 June

How to Sterilize Fish Tank and Equipment: Cleaning And Disinfecting The Tanks

By:

June 18, 2021

2 June

Zebrafish Husbandry 101: Processing & Fluorescent Screening

By:

June 2, 2021

3 May

Check it before you wreck it

By:

Stelian Pop ,

May 3, 2021

30 April

Zebrafish husbandry resources

By:

Kalyn Hubbard ,

April 30, 2021

21 April

Zebrafish Husbandry Basics: Breeding and Sexing [Video]

By:

Kalyn Hubbard ,

April 21, 2021

19 April

Key Considerations When Designing a Lifespan Assay

By:

Adam Saunders ,

April 19, 2021

10 April

Zebrafish Husbandry Basics: Breeding [Video]

By:

Kalyn Hubbard ,

April 10, 2021

9 April

4 Key Steps to Quality C. elegans Strain Confirmation

By:

Preston Kendrick ,

April 9, 2021

3 December

Zebrafish Genome Editing: CRISPR vs. Tol2

By:

Ashwin Suresh ,

December 3, 2020

One of our scientists compares two popular genome editing tools, CRISPR and Tol2 to better understand the pros and cons of each technology

20 November

Microfluidic EPG Recordings From Diverse Nematode Species

By:

Janis Weeks, Ph.D. ,

November 20, 2020

The InVivo Biosystem ScreenChip platform was developed for C. elegans, which is a free-living (non-parasitic) species that feeds on bacteria and has both hermaphroditic and

12 November

OP50 Vs. HB101: Which Food Should I Choose?

By:

Christine Kim ,

November 12, 2020

Nematode worms such as Caenorhabditis elegans feed on bacteria as a food source. In the wild, C. elegans obtain various bacteria from rotting plants, fruit,

3 November

A Spotlight on Recent Zebrafish Research

By:

November 3, 2020

We’ve compiled some of the most recent informative and influential research being done using the zebrafish model

21 October

How do I get rid of this pesky protein? The Hows and Whys of Protein Degradation

By:

October 21, 2020

Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.

20 October

How to identify a successful transgenic edit in C. elegans Part 1

By:

October 20, 2020

In this blog, we will discuss key factors to consider when choosing a screening method, introduce various editing techniques, and discuss the pros & cons

25 September

90 Mutations with 1 Gene: Functional Analysis of Variants Using C. elegans

By:

September 25, 2020

We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants

14 September

C. elegans CV

By:

September 14, 2020

1 September

Worms on drugs: How well can C. elegans predict drug toxicity in mammals?

By:

Adam Saunders ,

September 1, 2020

One can kill nematodes by feeding them any number of noxious chemicals, but how effectively can worms predict potential human toxicity of drug leads?

7 August

PCR Vs. HRMA For Genotyping: When To Use What?

By:

Preston Kendrick ,

August 7, 2020

3 August

Zebrafish CV

By:

August 3, 2020

25 June

Fluorescent Tagging Capability Demonstration

By:

June 25, 2020

At InVivo Biosystems, we have been working with fluorescent tags in C. elegans for over five years, and we have made over 600 fluorescent transgenic lines.

22 June

Research Spotlight: Alzheimer’s Disease

By:

June 22, 2020

June is Alzheimer’s & Brain Awareness Month. We wanted to take a few moments to spotlight some of the awe-inspiring research on Alzheimer’s disease taking

18 June

We Used CRISPR To Make 15 Point Mutations In 5 Genes. Here’s What We Learned.

By:

June 18, 2020

17 June

Zebrafish Injection Mix: Buy verses DIY, what are the real costs? [Infographic]

By:

June 17, 2020

Make sure you understand all of the costs associated with DIY-ing a zebrafish CRISPR project verses outsourcing it to a team with years of experience.

15 June

Why Validating Your Plasmids is Key to a Successful Transgenic, and How You Can Do It Too.

By:

Ashwin Suresh ,

June 15, 2020

Plasmids are a key tool in molecular biology. These circular pieces of DNA, also called “vectors” or “constructs”, can be engineered to contain large made-to-order

9 June

Seventeen Minutes of Science: From Zebrafish to Electric Fish, the Evolution of a PhD Student’s Research

By:

June 9, 2020

Tune in weekly to our virtual series “Seventeen Minutes of Science” every Tuesday at 11am PST / 2pm ET where we go live on Facebook

1 June

Precise Knock-Outs For Precise Research

By:

June 1, 2020

29 May

5 Simple Steps to Improve Your Injection Success

By:

Theresa Patch ,

May 29, 2020

We do injections everyday. While we’ve gotten pretty good at them, they don’t always go as planned. What does it mean if an injection has

20 May

A New School of Thought: Using Zebrafish to Understand Genetics of Group Behavior

By:

May 20, 2020

As we all spend time isolating ourselves for social distancing purposes, group behavior is on the forefront of our minds in new and unforeseen ways.

19 May

Seventeen Minutes of Science: Worming our way into Machine Learning (Episode 9)

By:

May 19, 2020

Stuck at home with nothing to do during this quarantine? Restless to still be learning and interacting with other scientists? You’re in luck! You can

14 May

When Details Matter: Bacterial Inactivation in Compound Associated Longevity Testing

By:

Tanner Feustel ,

May 14, 2020

One of our lab techs gives us an inside look at the development process of our automated Lifespan Analysis Platform and the importance of UV-inactivated

12 May

Seventeen Minutes of Science: What it Takes to Support Zebrafish Research (Episode 8)

By:

May 12, 2020

Stuck at home with nothing to do during this quarantine? Restless to still be learning and interacting with other scientists? You’re in luck! You can

30 April

11 Steps to a Successful CRISPR Knock-in

By:

April 30, 2020

Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in

20 April

[Webinar] A Shortcut To Understanding Longevity: A Rapid In Vivo Analysis Of A Compound’s Effect On Health And Aging

By:

April 20, 2020

16 April

Zebrafish Acute Toxicity Study Demonstrates Concordance With Rat Fetal Embryotoxicity

By:

April 16, 2020

8 April

The Challenge of Getting CRISPR-based Knock-ins to Work in Zebrafish

By:

Chris Hopkins ,

April 8, 2020

We take a look into why so many zebrafish groups struggle to achieve knock-in transgenesis and what makes knock-ins so difficult.

7 April

Seventeen Minutes of Science: The More Models the Merrier – Zebrafish & C. elegans Working Together (Episode 3)

By:

April 7, 2020

1 April

6 Things That Can Go Wrong While Making Your Zebrafish CRISPR Knock-In

By:

April 1, 2020

25 March

Seventeen Minutes of Science: Ben Loves Zebrafish & CRISPR (Episode 1)

By:

March 25, 2020

24 March

To truly validate your sgRNAs, go In Vivo

By:

Marnie Preston ,

March 24, 2020

CRISPR/Cas9 gene editing for Zebrafish is a rapidly growing molecular biology toolset with diverse applications, but is not without its technological barriers. Reagent sourcing, design

30 October

Where the Lead Leads: Seeking Mechanisms of Action in C. elegans for Neurotoxic Effects of Lead

By:

Janis Weeks, Ph.D. ,

October 30, 2019

Neurotoxin exposure is implicated in the etiology of neurodegenerative diseases including Parkinson’s disease, Alzheimer’s disease and ALS (amyotrophic lateral sclerosis). Traditionally, vertebrate animals such as

24 October

Featured Scientist: Ben Jussila (NemaMetrix)

By:

October 24, 2019

Ben Jussila graduated from the University of Minnesota (Twin Cities/Minneapolis) with a major in Genetics, Cell Biology & Development. Ben is currently an R&D Scientist

25 September

The Evolution of Drug Resistance; an Issue of “When, not if”

By:

Janis Weeks, Ph.D. ,

September 25, 2019

Drug resistance in many pathogens — viruses, bacteria, protozoa, parasitic worms and more — presents a growing and potentially catastrophic challenge to human and animal

24 September

Total Domination – Uncovering the Phenomenon of 1:2 Dominant vs Recessive ratio for Variation in the Genome

By:

Chris Hopkins ,

September 24, 2019

Our CSO revisits his Veritas Genomic Sequencing report for a closer look at how significant some of the discovered variants are

13 September

Using Zebrafish for Rapid Genetic Analysis

By:

September 13, 2019

11 September

Article Review: The Caenorhabditis elegans Transgenic Toolbox

By:

Sophia Bethel ,

September 11, 2019

We reviewed a recent article that discusses the best techniques available for C. elegans transgenics, as well as experimental concerns that should be considered.

15 August

C. elegans New Lab Checklist

By:

Sophia Bethel ,

August 15, 2019

Are you starting a new C. elegans lab and feeling overwhelmed with starting from scratch? We want to help make this process as smooth as

9 July

Featured Scientist: Adrian Wolstenholme (University of Georgia)

By:

July 9, 2019

Dr. Adrian Wolstenholme graduated from the University of London with a BSc in Biochemistry and has since spent almost 43 years working in the lab.

6 May

Conditional mutagenesis by loxP insertion in zebrafish – outfoxing the unfloxable

By:

May 6, 2019

CRISPR/Cas9-mediated integration of loxP sequences for conditional mutagenesis in zebrafish has recently made its way into the toolkit of zebrafish researchers. In particular, CRISPR/Cas9 mutagenesis

1 April

Featured Scientist: Anne Hart (Brown University)

By:

April 1, 2019

Anne Hart’s research focuses on fundamental problems in neuroscience using tools available in the nematode C. elegans. She received her Ph.D. in Neuroscience from UCLA,

4 March

The Unnecessary Procedure – A Problem with False Positives in Genomic Testing

By:

Chris Hopkins ,

March 4, 2019

There is a significant pressure to increase diagnostic yield and it has its consequences. BRCA testing is probably the most developed ecosystem for genetic tests

20 February

Meet Janis Weeks: A Neuroscientist Turned Global Health Advocate

By:

February 20, 2019

6 February

Using C. elegans to study parasitism

By:

Ramesh Ratnappan ,

February 6, 2019

C. elegans is one of the most powerful model organisms and has been used to unravel several key genetic pathways. The C. elegans nematode has been used

8 January

Featured Scientist: Sangeetha Iyer (Perlara PBC)

By:

January 8, 2019

Sangeetha Iyer is the director of preclinical development at Perlara. In her work, she is interested in demonstrating that nematodes can be used as model

18 December

A New Era of Transgenics in Parasitic Nematodes

By:

Ramesh Ratnappan ,

December 18, 2018

One of the most commonly known nematodes in the scientific community is Caenorhabditis elegans. Ever since Sydney Brenner introduced it as a model organism to

1 November

Featured Scientist: Shawn Lockery (University of Oregon, NemaMetrix)

By:

November 1, 2018

My academic research at the University of Oregon concerns the neuronal basis of behavior in one of the smallest brains known to science,

17 October

CRISPR/Cas9 off target effects: Hyperactive cutting or media hype?

By:

October 17, 2018

There has been a lot of media coverage in recent months surrounding CRISPR/Cas9 and the potentially life changing impact the gene-editing technology could have. More

wMicroTracker Applications: Mutant and Toxicity Screening

By:

October 9, 2018

The wMicroTracker is an essential tool to add to your mutant and toxicity screening work. Check out the application sheet below to see how easy

wMicroTracker Applications: Oxidative Stress Assays

By:

October 9, 2018

The wMicroTracker is an essential tool to add to your oxidative stress assays. Check out the application sheet below to see how easy it is

wMicroTracker Applications: Aging and Healthspan

By:

October 9, 2018

The wMicroTracker is a great product to add to your work around aging and healthpsan. Check out the application sheet below to see how easy

wMicroTracker Applications: Drug Screening

By:

October 9, 2018

The wMicroTracker is a great product to add to your drug screening toolkit. Check out the application sheet below to see how easy it is

3 October

Insertion of two loxP sites to produce a knockout (KO) of an embryonic lethal gene

By:

October 3, 2018

A client wanted a knockout (KO) of an embryonic lethal gene. We could not make this line using our standard methods. Instead, we inserted two

21 September

Analyzing Small C. elegans Populations with the wMicroTracker

By:

September 21, 2018

Most experiments are based on population analysis. However, some researchers are interested in the study of a single individual. In wMicrotracker it is possible to

7 May

Genome wide associations and the nematode pharynx: ever the twain shall meet

By:

Shawn Lockery, Ph.D., Co-founder ,

May 7, 2018

Question: What might you get if you cross the feeding organ of a roundworm with a genome wide association study? Answer: A cornucopia of novel

1 September

C. elegans in cancer research

By:

September 1, 2017

In recent years, the nematode C. elegans has emerged as a model for systematic dissection of the molecular basis of tumorigenesis. Many cancer genes and

24 August

Deciphering Breast Cancer with C. elegans – A Case Study

By: