The InVivo Biosystem ScreenChip platform was developed for C. elegans, which is a free-living (non-parasitic) species that feeds on bacteria and has both hermaphroditic and male sexes. However, C. elegans represents only one of the estimated 1 million members of the highly diverse phylum Nematoda, of which only ~25,000 species have been described. In addition to free-living species, other nematodes parasitize humans, animals or plants, with significant medical, veterinary and economic consequences. To demonstrate the utility of microfluidic EPG recordings from species beyond C. elegans, we adapted the platform to record from human and animal parasites, male and female members of a dioecious species, and a carnivorous nematode.
View from the Bench
Nematode worms such as Caenorhabditis elegans feed on bacteria as a food source. In the wild, C. elegans obtain various bacteria from rotting plants, fruit, and vegetation, but under laboratory conditions, C. elegans is normally cultured on agar plates with a standardized bacterial lawn. There are many bacterial diets used for culturing C. elegans. The …
Are you wanting to learn more about zebrafish husbandry? Have you ever wondered how we do our zebrafish crosses? Our zebrafish team put together this insightful video to help explain some of the zebrafish husbandry process, focusing on zebrafish crosses
We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants were selected from the ClinVar database, literature, the Gnomad database, clinical researchers, and the STXBP1 foundation.
When you have to apply for a new job, you are asked to provide a resume, CV, and a cover letter. So why then aren’t you asking the same of the model organisms you are using? We put together a fun, yet very informative, C. elegans CV for you to learn more about the small worm and why it makes an ideal model for studying human diseases.
One can kill nematodes by feeding them any number of noxious chemicals, but how effectively can worms predict potential human toxicity of drug leads? Also, given that the dosage is often the difference between a remedy or a poison, how well does the dosage of a drug translate between worms and humans? If worms lack most of the organ systems that would be key targets of drug toxicity, then how do we test toxicity in organ systems that the worms don’t have? We will discuss how InVivo Biosystems addresses these questions when using C. elegans as a model for drug testing.