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InVivo Biosystems Blog

Category: View from the Bench

Employee Spotlight: Technical Applications Scientist Ben Jussila

By:
Astha Nimavat ,
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Employee Spotlight: Lab Manager LeAnn Bowers

By:
Astha Nimavat ,
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Employee Spotlight: Project Manager Kalyn Hubbard

By:
Astha Nimavat ,
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Employee Spotlight: Senior Software Developer Kolt McBride

By:
Astha Nimavat ,
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How do we use Machine Learning to Automatically Measure C.elegans Lifespan?

By:
Kolt McBride ,
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Testing the efficacy of JadeAging®, a Chinese Medicine based longevity formulation, using InVivo Biosystems’ suite of in vivo platforms

By:
Alexandra Narin ,
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Temperature & C. elegans Part Two: How does Temperature affect development?

By:
Christine Kim ,
Thomas Adams ,
Summary: We recently published a piece on the importance of temperature in C.elegans which got the whole InVivio Biosystems team thinking about C. elegans’ development,
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The Benefits of Standardizing Zebrafish Diets

By:
Emma Lustig ,
Gianna DeMarco ,
Devon Scallion ,
We recently were asked to procure a special zebrafish diet, which got us thinking about zebrafish diets overall. Once we started researching this custom diet,
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A Day in the Life: Lab Tech I

By:
Marilee Hoyle ,
Summary: Marilee joined InVivo Biosystems in 2021 after graduating with a BSc in Environmental Science from OSU, in this article she chronicles a day in
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Mistakes and Lessons Learned Part 5: Maintaining Homozygous Cultures/Male Identification

By:
Dan Richards ,
LeAnn Bowers ,
Summary: In this five part series we are exploring some common mishaps when working with C. elegans, so you can hopefully avoid them too! Especially since
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Mistakes and Lessons Learned Part 3: Worms Over the Edge

By:
Dan Richards ,
LeAnn Bowers ,
In this article we will discuss the issues that arise with worms burrowing or crawling over the edge of the plate.
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How Do Knockins Work?

By:
Karl Stuntzner-Gibson ,
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Mistakes and Lessons Learned Part 2: Fungal and Bacterial Contamination

By:
Dan Richards ,
LeAnn Bowers ,
What can I do if I accidentally left a plate of worms uncovered on my bench and now there is a spot of contamination?
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Mistakes and Lessons Learned Part 1: Mistakes That Lead to Stress or Death

By:
Dan Richards ,
LeAnn Bowers ,
In this blog we will discuss ways worms may be stressed or killed unintentionally, such as being too rough or slow during worm transferring.
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Zebrafish 101: A White Paper

By:
Alexandra Narin ,
In this white paper we will discuss the advantages and limitations of zebrafish and ask: is a zebrafish model right for my research?
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C. elegans 101: A White Paper

By:
Alexandra Narin ,
In this whitepaper we discuss the advantages and limitations of C. elegans, and ask the question: is a C. elegans model right for my research?
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How does Cre/loxP recombination work?

By:
Ashwin Suresh ,
Jenn Lawson ,
In this blog, we discuss the basics of the Cre/Lox technique which is used in conditionally controlling gene manipulation in zebrafish.
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Why is temperature important for C. elegans?

By:
Trisha Brock ,
In this article we discuss the effect temperature has on our favorite worms, and how we can exploit these effects when designing studies.
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Considerations When Adding New Zebrafish Racks

By:
Emma Lustig ,
Devon Scallion ,
Gianna DeMarco ,
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Tips and Tricks for Successful Worm Picking

By:
Laura Taggart-Murphy ,
Christine Kim ,
Dan Richards ,
Alexandra Narin ,
When working with C. elegans it is vital that you can deftly handle them. Worm picking, however, is a skill where practice makes perfect. At
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What is the new Split hygromycin method? How does it differ from MosSCI? How can we use it to help you?

By:
Lauren Resch ,
Alexandra Narin ,
Marilee Hoyle ,
Anna Malinkevich, MS ,
In 2020 a new method of inserting transgenes into the genome of C, elegans was introduced to the field, and was quickly adopted by InVivo
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Which Popular Live Food is Best for Your Zebrafish? A Head-to-Head Comparison of Rotifers and Artemia

By:
Madeleine Bunnenberg-Ross ,
When choosing the right food for a Danio rerio colony there are a number of factors to consider. This guide will help navigate the roadblocks
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Zebrafish Husbandry 101: Crossing Basics

By:
InVivo Biosystems ,
This video provides a brief overview of the zebrafish crossing process that we utilize in-house at InVivo Biosystems
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Zebrafish Husbandry 101: Embryo Injections

By:
InVivo Biosystems ,
Learn how to perform a zebrafish embryo injection and some tips and tricks so you can ace your next injections too.
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Zebrafish Husbandry 101: Maintaining Water Quality

By:
InVivo Biosystems ,
Maintaining water quality is crucial for successful zebrafish maintenance and husbandry, especially during the startup of a fish rack. This video discusses how to keep
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Zebrafish Husbandry 101: Tracking Fish Health

By:
InVivo Biosystems ,
This video discusses the daily maintenance and upkeep of zebrafish, including keeping track of population health, our daily zebrafish rack checklist (water flow, buffer fills,
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Zebrafish Husbandry 101: Live Food vs. Dry Food

By:
InVivo Biosystems ,
Have you ever wondered what type of food to feed your zebrafish? This video highlights what we choose to feed our fish and why
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Zebrafish Husbandry 101: Cleaning and Disinfecting the Tanks

By:
InVivo Biosystems ,
An important, but time-consuming, aspect of zebrafish maintenance and husbandry is the proper cleaning and disinfecting of lab equipment. Tanks should be cleaned every 5-6
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Validating Expression of Humanizations – What We’ve Done so Far

By:
Jenn Lawson ,
In an effort to create better research models for epilepsy, we’ve been working on a grant-funded project to humanize numerous C. elegans genes. Learn more
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Zebrafish Husbandry 101: Processing & Fluorescent Screening

By:
InVivo Biosystems ,
How can you better study your gene of interest? Fluorescent tagging! This video showcases InVivo Biosystems’ fluorescent screening process using zebrafish. Watch the video
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Problem Solved: Using a new technique to solve a problem

By:
Jenn Lawson ,
Two main lessons learned: 1. stay current with a broad variety of literature and 2. always be willing to try new methods
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Check it before you wreck it

By:
Stelian Pop ,
Mistakes can happen, various design issues can be overlooked, or maybe just a less than optimal design was used, so how do we at InVivo
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Zebrafish husbandry resources

By:
Kalyn Hubbard ,
Visual learners rejoice! We’ve compiled a list of great resources for you to learn everything you want to know about zebrafish husbandry.
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Zebrafish Husbandry Basics: Breeding and Sexing [Video]

By:
Kalyn Hubbard ,
Emma Lustig ,
Are you wanting to learn more about zebrafish breeding? Have you ever wondered how to tell the males from the females when they’re all swimming
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Key Considerations When Designing a Lifespan Assay

By:
Adam Saunders ,
Alexandra Narin ,
Developing an analysis strategy up front in parallel with experimental design is key to avoiding costly errors down the road.
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Zebrafish Husbandry Basics: Breeding [Video]

By:
Kalyn Hubbard ,
Emma Lustig ,
Are you wanting to learn more about zebrafish husbandry? Have you ever wondered how we do our zebrafish crosses? Our zebrafish team put together this
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4 Key Steps to Quality C. elegans Strain Confirmation

By:
Preston Kendrick ,
How do we ensure that we deliver high quality C. elegans strains to our clients so that they are confident in the transgenic animals they
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Egg Viability Assay – A View From The Bench

By:
Ashwin Suresh ,
The main purpose of this assay is to find the potential humanized LMNA worm variants that have a fecundity defect.
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Zebrafish Genome Editing: CRISPR vs. Tol2

By:
Ashwin Suresh ,
One of our scientists compares two popular genome editing tools, CRISPR and Tol2 to better understand the pros and cons of each technology
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Microfluidic EPG Recordings From Diverse Nematode Species

By:
Janis Weeks, Ph.D. ,
The InVivo Biosystem ScreenChip platform was developed for C. elegans, which is a free-living (non-parasitic) species that feeds on bacteria and has both hermaphroditic and
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OP50 Vs. HB101: Which Food Should I Choose?

By:
Christine Kim ,
Nematode worms such as Caenorhabditis elegans feed on bacteria as a food source. In the wild, C. elegans obtain various bacteria from rotting plants, fruit,
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A Spotlight on Recent Zebrafish Research

By:
Ben Jussila ,
We’ve compiled some of the most recent informative and influential research being done using the zebrafish model
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How do I get rid of this pesky protein? The Hows and Whys of Protein Degradation

By:
Trisha Brock ,
Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.
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How to identify a successful transgenic edit in C. elegans Part 1

By:
Lauren Resch ,
In this blog, we will discuss key factors to consider when choosing a screening method, introduce various editing techniques, and discuss the pros & cons
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90 Mutations with 1 Gene: Functional Analysis of Variants Using C. elegans

By:
Trisha Brock ,
Kathryn McCormick ,
We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants
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C. elegans CV

By:
Hannah Huston ,
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Worms on drugs: How well can C. elegans predict drug toxicity in mammals?

By:
Adam Saunders ,
One can kill nematodes by feeding them any number of noxious chemicals, but how effectively can worms predict potential human toxicity of drug leads?
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The Need for Animal Models in COVID-19 Research

By:
InVivo Biosystems ,
Our CSO recently helped write a letter expressing the need for animal models in COVID-19 research
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PCR Vs. HRMA For Genotyping: When To Use What?

By:
Preston Kendrick ,
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Research Spotlight: Summer Sun Safety Month

By:
Jenn Lawson ,
C. elegans and Zebrafish have been used to study the effects of ultraviolet radiation (UVR) for decades. August is Summer Sun Safety Month so in
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Zebrafish CV

By:
Hannah Huston ,
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Worms, They’re Just Like Us! [Infographic]

By:
Hannah Huston ,
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Fluorescent Tagging Capability Demonstration

By:
Lauren Resch ,
At InVivo Biosystems, we have been working with fluorescent tags in C. elegans for over five years, and we have made over 600 fluorescent transgenic lines.
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A View From the Bench: Roadblocks and Frustrations When Expectation Meets Reality.

By:
Jenn Lawson ,
In an effort to create better models for epilepsy, we’ve been working on a grant-funded project to humanize important synaptic machinery by replacing endogenous coding
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Research Spotlight: Alzheimer’s Disease

By:
Kathryn McCormick ,
June is Alzheimer’s & Brain Awareness Month. We wanted to take a few moments to spotlight some of the awe-inspiring research on Alzheimer’s disease taking
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We Used CRISPR To Make 15 Point Mutations In 5 Genes. Here’s What We Learned.

By:
Trisha Brock ,
Kathryn McCormick ,
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Zebrafish Injection Mix: Buy verses DIY, what are the real costs? [Infographic]

By:
InVivo Biosystems ,
Make sure you understand all of the costs associated with DIY-ing a zebrafish CRISPR project verses outsourcing it to a team with years of experience.
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Why Validating Your Plasmids is Key to a Successful Transgenic, and How You Can Do It Too.

By:
Ashwin Suresh ,
Plasmids are a key tool in molecular biology. These circular pieces of DNA, also called “vectors” or “constructs”,  can be engineered to contain large made-to-order
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Seventeen Minutes of Science: From Zebrafish to Electric Fish, the Evolution of a PhD Student’s Research

By:
InVivo Biosystems ,
Tune in weekly to our virtual series “Seventeen Minutes of Science” every Tuesday at 11am PST / 2pm ET where we go live on Facebook
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Precise Knock-Outs For Precise Research

By:
Lauren Resch ,
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5 Simple Steps to Improve Your Injection Success

By:
Theresa Patch ,
We do injections everyday. While we’ve gotten pretty good at them, they don’t always go as planned. What does it mean if an injection has
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A New School of Thought: Using Zebrafish to Understand Genetics of Group Behavior

By:
Ben Jussila ,
As we all spend time isolating ourselves for social distancing purposes, group behavior is on the forefront of our minds in new and unforeseen ways.
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Seventeen Minutes of Science: Worming our way into Machine Learning (Episode 9)

By:
InVivo Biosystems ,
Stuck at home with nothing to do during this quarantine? Restless to still be learning and interacting with other scientists? You’re in luck! You can
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When Details Matter: Bacterial Inactivation in Compound Associated Longevity Testing

By:
Tanner Feustel ,
One of our lab techs gives us an inside look at the development process of our automated Lifespan Analysis Platform and the importance of UV-inactivated
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Seventeen Minutes of Science: What it Takes to Support Zebrafish Research (Episode 8)

By:
InVivo Biosystems ,
Stuck at home with nothing to do during this quarantine? Restless to still be learning and interacting with other scientists? You’re in luck! You can
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The Importance of COVID-19 Environmental Testing

By:
Sandra Torres ,
Originally posted on the Enviral Tech website. Read it on their site here. Why you should start testing your facilities for COVID-19 now As COVID-19
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11 Steps to a Successful CRISPR Knock-in

By:
Trisha Brock ,
Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in
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6 Key Steps to a Successful Zebrafish Knock-in [Infographic]

By:
Hannah Huston ,
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[Webinar] A Shortcut To Understanding Longevity: A Rapid In Vivo Analysis Of A Compound’s Effect On Health And Aging

By:
Hannah Huston ,
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Zebrafish Acute Toxicity Study Demonstrates Concordance With Rat Fetal Embryotoxicity

By:
Kathryn McCormick ,
Ben Jussila ,
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The Challenge of Getting CRISPR-based Knock-ins to Work in Zebrafish

By:
Chris Hopkins ,
Kathryn McCormick ,
We take a look into why so many zebrafish groups struggle to achieve knock-in transgenesis and what makes knock-ins so difficult.
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Seventeen Minutes of Science: The More Models the Merrier – Zebrafish & C. elegans Working Together (Episode 3)

By:
Hannah Huston ,
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6 Things That Can Go Wrong While Making Your Zebrafish CRISPR Knock-In

By:
Ben Jussila ,
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Seventeen Minutes of Science: Ben Loves Zebrafish & CRISPR (Episode 1)

By:
Hannah Huston ,
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To truly validate your sgRNAs, go In Vivo

By:
Marnie Preston ,
CRISPR/Cas9 gene editing for Zebrafish is a rapidly growing molecular biology toolset with diverse applications, but is not without its technological barriers. Reagent sourcing, design
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Intro to CRISPR: Zebrafish Mutations [Infographic]

By:
Hannah Huston ,
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Where the Lead Leads: Seeking Mechanisms of Action in C. elegans for Neurotoxic Effects of Lead

By:
Janis Weeks, Ph.D. ,
Neurotoxin exposure is implicated in the etiology of neurodegenerative diseases including Parkinson’s disease, Alzheimer’s disease and ALS (amyotrophic lateral sclerosis). Traditionally, vertebrate animals such as
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Featured Scientist: Ben Jussila (NemaMetrix)

By:
InVivo Biosystems ,
Ben Jussila graduated from the University of Minnesota (Twin Cities/Minneapolis) with a major in Genetics, Cell Biology & Development. Ben is currently an R&D Scientist
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The Evolution of Drug Resistance; an Issue of “When, not if”

By:
Janis Weeks, Ph.D. ,
Drug resistance in many pathogens — viruses, bacteria, protozoa, parasitic worms and more — presents a growing and potentially catastrophic challenge to human and animal
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Total Domination – Uncovering the Phenomenon of 1:2 Dominant vs Recessive ratio for Variation in the Genome

By:
Chris Hopkins ,
Our CSO revisits his Veritas Genomic Sequencing report for a closer look at how significant some of the discovered variants are
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Using Zebrafish for Rapid Genetic Analysis

By:
Hannah Huston ,
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Article Review: The Caenorhabditis elegans Transgenic Toolbox

By:
Sophia Bethel ,
We reviewed a recent article that discusses the best techniques available for C. elegans transgenics, as well as experimental concerns that should be considered.
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A Look Inside the CRISPR Toolbox

By:
Sophia Bethel ,
This visual quick-guide offers a look inside the control possible through CRISPR genome edited transgenics, and will offer guidance as to which method would be
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C. elegans New Lab Checklist

By:
Sophia Bethel ,
Are you starting a new C. elegans lab and feeling overwhelmed with starting from scratch? We want to help make this process as smooth as
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Ask An Expert: The importance of experimental assay or analysis temperatures in C. elegans studies

By:
Anna Malinkevich, MS ,
Note: We no longer sell the Arena Instrument, so please check out our updated article on the importance of temperature in C. elegans here to
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Featured Scientist: Adrian Wolstenholme (University of Georgia)

By:
InVivo Biosystems ,
Dr. Adrian Wolstenholme graduated from the University of London with a BSc in Biochemistry and has since spent almost 43 years working in the lab.
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Conditional mutagenesis by loxP insertion in zebrafish – outfoxing the unfloxable

By:
Ben Jussila ,
CRISPR/Cas9-mediated integration of loxP sequences for conditional mutagenesis in zebrafish has recently made its way into the toolkit of zebrafish researchers. In particular, CRISPR/Cas9 mutagenesis
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C. elegans showing its weight in neuro-toxicology research

By:
,
Hannah Huston ,
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Featured Scientist: Anne Hart (Brown University)

By:
InVivo Biosystems ,
Anne Hart’s research focuses on fundamental problems in neuroscience using tools available in the nematode C. elegans. She received her Ph.D. in Neuroscience from UCLA,
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The Unnecessary Procedure – A Problem with False Positives in Genomic Testing

By:
Chris Hopkins ,
There is a significant pressure to increase diagnostic yield and it has its consequences. BRCA testing is probably the most developed ecosystem for genetic tests
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Meet Janis Weeks: A Neuroscientist Turned Global Health Advocate

By:
Hannah Huston ,
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Using C. elegans to study parasitism

By:
Ramesh Ratnappan ,
C. elegans is one of the most powerful model organisms and has been used to unravel several key genetic pathways. The C. elegans nematode has been used
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Ask an expert: What is the difference between the Worminator and the wMicroTracker?

By:
Yoanne Clovis, Ph.D. ,
Buying a product is no longer as easy as looking it up and purchasing it. Instead, we spend additional time “price-shopping” and comparing the features
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Featured Scientist: Sangeetha Iyer (Perlara PBC)

By:
InVivo Biosystems ,
Sangeetha Iyer is the director of preclinical development at Perlara. In her work, she is interested in demonstrating that nematodes can be used as model
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A New Era of Transgenics in Parasitic Nematodes

By:
Ramesh Ratnappan ,
One of the most commonly known nematodes in the scientific community is Caenorhabditis elegans. Ever since Sydney Brenner introduced it as a model organism to
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Dealing with Uncertain Significance of the Genome

By:
Chris Hopkins ,
“Risky Business” Most of us are wired to be risk averse. Yet, I have been giving serious contemplation to the “risky business” of having my whole
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Featured Scientist: Shawn Lockery (University of Oregon, NemaMetrix)

By:
InVivo Biosystems ,
    My academic research at the University of Oregon concerns the neuronal basis of behavior in one of the smallest brains known to science,
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CRISPR/Cas9 off target effects: Hyperactive cutting or media hype?

By:
Ben Jussila ,
There has been a lot of media coverage in recent months surrounding CRISPR/Cas9 and the potentially life changing impact the gene-editing technology could have. More
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wMicroTracker Applications: Mutant and Toxicity Screening

By:
Yoanne Clovis, Ph.D. ,
The wMicroTracker is an essential tool to add to your mutant and toxicity screening work. Check out the application sheet below to see how easy
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wMicroTracker Applications: Oxidative Stress Assays

By:
Yoanne Clovis, Ph.D. ,
The wMicroTracker is an essential tool to add to your oxidative stress assays. Check out the application sheet below to see how easy it is
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