Are you wanting to learn more about zebrafish breeding? Have you ever wondered how to tell the males from the females when they’re all swimming around? Our zebrafish team put together this insightful video to show the process of breeding zebrafish and sexing.
View from the Bench
The InVivo Biosystem ScreenChip platform was developed for C. elegans, which is a free-living (non-parasitic) species that feeds on bacteria and has both hermaphroditic and male sexes. However, C. elegans represents only one of the estimated 1 million members of the highly diverse phylum Nematoda, of which only ~25,000 species have been described. In addition to free-living species, other nematodes parasitize humans, animals or plants, with significant medical, veterinary and economic consequences. To demonstrate the utility of microfluidic EPG recordings from species beyond C. elegans, we adapted the platform to record from human and animal parasites, male and female members of a dioecious species, and a carnivorous nematode.
Nematode worms such as Caenorhabditis elegans feed on bacteria as a food source. In the wild, C. elegans obtain various bacteria from rotting plants, fruit, and vegetation, but under laboratory conditions, C. elegans is normally cultured on agar plates with a standardized bacterial lawn. There are many bacterial diets used for culturing C. elegans. The …
Are you wanting to learn more about zebrafish husbandry? Have you ever wondered how we do our zebrafish crosses? Our zebrafish team put together this insightful video to help explain some of the zebrafish husbandry process, focusing on zebrafish crosses
We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants were selected from the ClinVar database, literature, the Gnomad database, clinical researchers, and the STXBP1 foundation.