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InVivo Biosystems specializes in providing customized transgenic services and gene editing services tailored to the specific research needs of its clients. The company’s team of experts has extensive experience in designing, constructing, and delivering custom reagents or genetically edited Caenorhabditis elegans strains optimized to clients’ specifications. Close collaboration with clients ensures a clear understanding of their research goals and the provision of transgenic worms that are best suited for their experiments.
With over 3,000 successfully delivered transgenic lines, clients can be confident in receiving top-quality results, facilitating data generation, publication of results, and the development of affordable therapeutics for rare diseases.
InVivo Biosystems is an officially licensed CRISPR solutions provider and experts in C. elegans genome editing. The company’s unique internal processes, including plasmid validation, injection protocol, and editing efficiency optimization, ensure the delivery of high-quality strains that meet clients’ research needs.
Understanding gene function: With C. elegans genome editing, researchers can manipulate specific genes and study their effects on the organism. This allows for a deeper understanding of gene function and the underlying mechanisms of biological processes.
Disease modeling: C. elegans can serve as a model organism for human diseases due to its genetic similarity. By editing the genome of C. elegans, researchers can create disease models to study the disease mechanisms and test potential treatments.
Drug discovery: Genome editing in C. elegans can also be used in the discovery of new drugs or the evaluation of existing ones. By targeting specific genes, researchers can identify potential drug targets and test the efficacy of drugs in treating diseases.
Evolutionary biology: The use of C. elegans genome editing can also aid in understanding evolutionary biology. By studying changes in the genome over time, researchers can gain insights into the evolution of species.
Biotechnology: C. elegans can be used in biotechnology applications such as the production of recombinant proteins. Genome editing can be used to enhance these processes and improve their efficiency.
We work with you on project design using a collaborative approach to ensure that you receive the C. elegans line best suited for your experiments.
InVivo Biosystems offers various C. elegans service packages that are designed to meet the unique needs and budget of every laboratory.
The table below highlights the features and benefits of each package.
We collaborate with you to understand your gene expression expectations. This allows us to find the most... optimal promoter, coding, and terminator sequences to maximize the chance of successful strain generation. We listen to your needs and adapt the project accordingly. See more
Depending on the project approach, we often turn to de novo sequence generation through our suppliers... Alternatively, we can deploy a variety of gene assembly approaches to get the exact sequence needed for appropriate tissue-specific expression. Either way, we efficiently generate the sequence components needed for creation of high efficiency injection mix components. See more
With thousands of lines delivered, our team of injectionist have the necessary skill. They can ... obtain precision delivery of transgenesis reagents to the nematode gonad. Often, only a few animals need to be injected in order to generate multiple biological replicates. See more
Often our process has a selection marker that helps us identify candidates, but we don't stop there. For our ... genomic integrants, we look at each transgenesis junction by PCR. Once we confirm the ends of the gene insertion have proceeded as planned, clients can opinion for full sequencing of the entire transgene sequence so that a sequence verified strain is delivered. See more
We perform a sufficient number of injections that typically 3 or more biological replicates ... are generated. Occasionally the numbers of animals transformed is below this number in a set of injected animals. When this is detected, we quickly redo another set of injections to ensure that you received a good chance at multiple biological replicate strains. See more
At InVivo Biosystems, a strong emphasis is placed on quality assurance to ensure client satisfaction. When clients choose to work with InVivo Biosystems, they can expect a commitment to quality throughout their project.
Consultation with our genome engineering design experts to build the exact custom transgenic you need for your research.
Bi-weekly updates about the progress made on your project from our team of genome engineers. Key milestones will be noted.
Receive a validation report that describes the details of your genome edit and shows the evidence that the desired edit was made.
The transparent billing practices at InVivo Biosystems ensure that clients have a clear understanding of the costs involved in the project.
Full Build and Candidate Lines Projects
InVivo Biosystems bills on a 50/50 service payment plan for Full Build and Candidate Lines projects. The first 50% is required for project start and is non-refundable. The second 50% is paid at end of the project and is only billable if a line is delivered. Prices stated are exclusive of all taxes, fees, licenses, duties or levies and, unless otherwise stated in the quotation, transportation charges, freight and insurance.
Custom Injection Mix and Inject Express Projects
Payment in full is due for Custom Injection Mix and Inject Express project start and is non-refundable. InVivo Biosystems quotations are valid for thirty (30) days unless otherwise stated in the quotation. Prices stated are exclusive of all taxes, fees, licenses, duties or levies and, unless otherwise stated in the quotation, transportation charges, freight and insurance.
At the request of WormBase, genetic variation generated and recovered by InVivo Biosystems should retain the “knu # “ allele designation provided. Allele names can be ascertained from either product shipping documents or by emailing InVivo Biosystems . For example: The strain gene-1(knu123[GFP::gene-1]) was generated by InVivo Biosystems. Get started with our genome-edited C. elegans services today and propel your research to new heights. Fill up the form below to discuss your project requirements with our team of experts.
The transparent billing practices at InVivo Biosystems ensure that clients have a clear understanding of the costs involved in the project.
InVivo Biosystems has successfully delivered 3,000 C. elegans transgenic lines to researchers who rely on our genome-edited strains to generate data and publish results. As an expert in C. elegans genome editing, we have developed many unique internal processes to ensure the quality of the strains, including plasmid validation, injection protocol, and to improve our editing efficiency. Choices of genome editing methods are CRISPR, MosSCI and Extrachromosomal Array.
C. elegans has become a key model for understanding the molecular mechanisms involved in a growing number of human diseases. Having a short generation time along with invariant developmental lineage, C. elegans has proved to be extremely beneficial for understanding basic cell biology, neurobiology and aging. Another unique advantage of C. elegans over other multicellular model organisms is that individuals can be frozen indefinitely and revived to obtain an F2 generation within a week, thus eliminating the cost of maintaining colonies of mutants
Yes, our strain and allele designation have been confirmed by the WormBase, and any genetic variation generated and recovered by InVivo Biosystems should retain the designated “knu # “ allele name. Allele names can be ascertained from either product shipping documents or by emailing InVivo Biosystems . For example: The strain gene-1 (knu123[GFP::gene-1]) was generated by InVivo Biosystems
We enable you to tag your favorite gene at the native locus via CRISPR/Cas9 gene editing technology choosing from a large selection of fluorescent proteins, immunotags and degron tags. Custom tags and combinations (i.e. GFP::3xFLAG, etc.) are also available.
InVivo Biosystems has the capability to make specific DNA alterations including knockout, point mutation, precise deletion, and floxed allele with high precision which enables you to study gene function in exciting new ways.
We offer MosSCI and Extrachromosomal array with varying cost and delivery time. Please view our package details for further information.
Yes, we offer custom injection services where you provide the gene editing idea in your strain receive professionally prepared injection mix containing all of the required molecular components to achieve your desired custom genetic edit. You will, however, require access to microinjection rig, technical skills for proper injection, and the ability to screen and select final desired strain(s).
Yes, we offer custom injection services where you provide the gene editing idea in your strain receive professionally prepared injection mix containing all of the required molecular components to achieve your desired custom genetic edit. You will, however, require access to microinjection rig, technical skills for proper injection, and the ability to screen and select final desired strain(s).
We offer a 50/50 service payment plan for Full Build and Candidate Lines projects. The first 50% is required for project start and is non-refundable. The second 50% is paid at end of the project and is only billable if a line is delivered.
Prices stated are exclusive of all taxes, fees, licenses, duties or levies and, unless otherwise stated in the quotation, transportation charges, freight and insurance.
InVivo Biosystems quotations are valid for thirty (30) days unless otherwise stated in the quotation. Please report within the mentioned duration.
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An expert in CRISPR genome editing, InVivo Biosystems creates custom genome-edited C. elegans and zebrafish models to enable aging, developmental, and disease studies. Our unique in vivo platforms and technologies bridge the gap between cells and mice.
