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Zebrafish CRISPR Injection Mix with sgRNA Validation

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All the Power of CRISPR: Complete CRISPR Injection Mix & sgRNA Validation

CRISPR/Cas9 gene editing is a rapidly growing molecular biology toolset with diverse applications. However, designing and executing a CRISPR project turns out to be more complex than it appears from the outset. Reagent sourcing, and design and validation can be a time intensive process requiring strong internal expertise without a guarantee of success.

We know you have tight deadlines, and may have limited hands or expertise. Our goal is to help you get the tools you need to move forward with your experiments and accelerate the time from your idea to execution of the project. You can work with our team of genome editing specialists to design your injection mix so all you have to do is add water, inject and start screening!

Zebrafish Injection Mix: Comparing Buy vs. DIY.

Is CRISPR Injection Mix the right choice for you?

Our CRISPR injection mixes are fully customizable to get you the edit you want along with the support you need to start your project. Our injection mix packages range from standard ready-to-inject mixes to full in vivo evaluation of sgRNA cutting efficiency and in house validated screening tools to accommodate YOUR level of expertise. Our Standard Mix is for experienced researchers with extensive injection and screening competencies; the Complete Validated Mix is a rigorously tested injection mix for researchers looking for a fully plug and play format.

 

To mitigate failure rates, our custom injection mix includes an expertly designed set of reagents and tools that can help you:

Customer Story

Images below show two different zebrafish lines using the CRISPR Injection Mix. (Left): SelN-BFPSTOP with BFP followed by a STOP codon inserted into the N-terminus of Selenon (SelN). (Center and Right): Ryr1a-mCherry line where the endogenous Ryr1a was tagged with mCherry at the N-terminus. Image courtesy of Melissa A. Wright, MD/PhD, Assistant Professor of Pediatric Neurology at University of Colorado.

SelN-BFPSTOP line generated using CRISPR injection mix.. A ventral view of 7-day post-fertilization zebrafish larvae with blue fluorescent protein expression in several multiple facial structures and in the ear. Image courtesy of Melissa A. Wright, MD/PhD, Assistant Professor of Pediatric Neurology at University of Colorado.
Visualizing slow muscle fibers in trunk muscle.. The images show mCherry expression in slow muscle fibers in trunk muscle at different magnifications (lateral view). Using CRISPR injection mix, the subcellular localization of the protein is easily visible in the higher magnification image. Image courtesy of Melissa A. Wright, MD/PhD, Assistant Professor of Pediatric Neurology at University of Colorado.
Visualizing slow muscle fibers in trunk muscle. The images show mCherry expression in slow muscle fibers in trunk muscle at different magnifications (lateral view). Using CRISPR injection mix, the subcellular localization of the protein is easily visible in the higher magnification image. Image courtesy of Melissa A. Wright, MD/PhD, Assistant Professor of Pediatric Neurology at University of Colorado.

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In order to use our injection mix, you must have access to:

Injection Mix and sgRNA Validation Offerings

Looking for an easy way to bring CRISPR gene editing into your lab? Choose the level of service that is appropriate for you.

Standard Injection Mix

Validated sgRNA Injection Mix

Fully Validated Injection Mix

Design

Injection Mix

Screening Reagents

In vivo sgRNA Testing

In vivo Editing Assesment

*Prices reflect academic pricing.
*Pricing is for point mutation and knockout projects. Projects that require plasmid builds adds $2,000 and 4-6 weeks to each project.
*In-depth consultation with our expert CRISPR designers and editors can be included with any project.

What do I need?

In order to use our injection mix, you must have access to:

Injection Mix and sgRNA Validation Details

Standard Injection Mix

Choose this option if you want us to pick your sgRNA site, have already tested your sgRNA in vivo, or want to make a CRISPR knockout. We send a custom designed injection mix and you develop your own preferred detection/screening protocol.

  • I have an experimental idea, but not sure how to proceed
  • I know the data I’d like to get, but am not sure what tool to use
  • I’d like to talk to an expert

Standard Injection Mix + Screening Primers

Choose this option if you want to inject and screen for your edit by PCR without having to worry about designing your own screening primers. This option includes our standard mix plus ready-to-go screening primers.

  • I have an experimental idea, but not sure how to proceed
  • I know the data I’d like to get, but am not sure what tool to use
  • I’d like to talk to an expert

Validated sgRNA Injection Mix

Choose this option if you want us to guarantee your injection mix is designed around a high efficiency sgRNA site using our rigorous in-house testing process.

  • I have an experimental idea, but not sure how to proceed
  • I know the data I’d like to get, but am not sure what tool to use
  • I’d like to talk to an expert

Complete Validated Injection Mix

Choose this option if you want a CRISPR knockin with completely validated reagents to use in your lab and to find your gene edited line. This is the best Custom Injection Mix option for the novice zebrafish line builder.

  • I have an experimental idea, but not sure how to proceed
  • I know the data I’d like to get, but am not sure what tool to use
  • I’d like to talk to an expert

Injection Mix Service Component Descriptions

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