Home › CRISPR Model Creation › Zebrafish Genome-Editing Services › Zebrafish Tol2 Transgenesis Services
Insert large content in the zebrafish genome using the Tol2 transposon-based system.
Using Tol2 transgenesis you can generate:
Tol2 Transposase
Random insertion
Multiple
Yes
10% to 40%
Transgenesis
Mosaic
Usually 13-15kb - depends on cargo
CRISPR/ Cas9
Site specific
Single
Yes
~2% to 5%
Knock-in Edit
Mosaic
Unknown but appears that the larger the insert the lower the efficiency.
Tol2 Plasmid Prep
Tol2 Plasmid Validation
Mosaic Clutch* (F0 injected embryos)
Full Build** (Stable transgenic line)
Tol2 Plasmid Design
Plasmid Assembly
InVivo Integration Assessment
Expertly Injected Embryos
Germline Transmission Screening & Line Propagation
* This service is only available to clients in the United States.
** Full Build includes screening n=100 adults; standard husbandry charge applies.
# Additional charge applies for cargo over 2kb in size for all service packages.
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