Our Neurotoxicity Platform using zebrafish measures behavioral characteristics of individual zebrafish larvae in response to potential neurotoxicants. This is an automated phenotypic characterization, performed with the DanioVision instrumentation from Noldus Labs, that can detect potential effects of neurotoxicity such as attenuated response to stimuli.
This assay is available in 96-well high throughput or in 24-well high resolution formats (figure 3).
Age-matched animals from at least two different groups are compared. The animals are recorded in response to stimuli such as light/dark cycling and vibration. The software identifies and tracks animals to quantify many aspects of behavior, including:
The DanioVision software tracks the behavior of individual zebrafish larvae over time as they explore an arena.
Fig. 2: Our neurotoxicant assay also tests for behavioral response to stimuli, such as light or dark periods. In this example, zebrafish treated with CuSO4 respond more significantly to a dark stimulus than their untreated siblings.
Fig. 3: Our assay measures the behavioral responses to potential neurotoxic compounds in several multi-well formats.
Fig. 4: Neurotoxicant effects on velocity. Acute treatment with 2% ethanol (blue) significantly increased zebrafish swimming velocity relative to vehicle control treatment (gray), whereas acute treatment with 8 µM CuSO4 (purple) and 1 µM AgNO3 (yellow) did not alter zebrafish swimming velocity relative to vehicle control treatment. ****, P < .0001.
Fig. 5: Neurotoxicant effects on movement following dark stimulus. Acute treatment with 2% ethanol (blue), 8 µM CuSO4 (purple), and 1 µM AgNO3 (yellow) significantly increased the distance that zebrafish moved following the application of a full-field dark stimulus, relative to vehicle control treatment (gray). *, P < .05; **, P < .01; ****, P < .0001.