fbpx
Contact Us

InVivo Biosystems Blog

Category: Gene Editing

WHAM with CRISPR - re-imaging how rare disease therapeutics are developed

Whole-gene Humanized Animal Models (WHAM) with CRISPR – Re-imagining how Rare Disease therapeutics are developed

By:
Chris Hopkins ,
Alexandra Narin ,
Traditional modeling of patient variants in vivo has two limitations which can be overcome utilizing InVivo Biosystems’ patented WHAM-method.
READ MORE

America is finally investing in Biotechnology & Biomanufacturing in a Big Way

By:
Alexandra Narin ,
In this article we discuss what President Biden’s New Executive Order means for America’s place in the biotech and biomanufacturing fields.
READ MORE

Looking at Gene Paralogs across both zebrafish and C. elegans Model Organisms [Customer Story]

By:
Alexandra Narin ,
Drs Pruyne & Almack leveraged InVivo Biosystems’ Custom Injection Mix to design and create a novel zebrafish line.
READ MORE

Review: How a Small Worm is Helping Uncover the Pathogenicity of Gene Variants

By:
Ben Jussila ,
Alexandra Narin ,
READ MORE

Differences Between HDR vs NHEJ

By:
Chesley Johnson ,
Summary The CRISPR-Cas9 system has become integral in the toolbelts of genomic scientists, researchers, students, etc. Never has it been so easy to wield a
READ MORE

How Do Knockins Work?

By:
Karl Stuntzner-Gibson ,
READ MORE

The ABCs of Genetic Research

By:
Alexandra Narin ,
Here at InVivo Biosystems we spend a lot of time thinking, and talking, about genome editing. We thought it would be fun to compile a
READ MORE

Does everyone have digestive issues? — Gut dysbiosis is on the rise.

By:
Alexandra Narin ,
In this article we discuss the rise in gut dysbiosis, and new risk factors: microplastics, consumption of supplements, and chronic stress.
READ MORE

How does Cre/loxP recombination work?

By:
Ashwin Suresh ,
Jenn Lawson ,
In this blog, we discuss the basics of the Cre/Lox technique which is used in conditionally controlling gene manipulation in zebrafish.
READ MORE

What is Zebrafish Knock-in Tagging?

By:
Ben Jussila ,
With knock-in tagging, you can tag your favorite gene at the native locus in your zebrafish line via CRISPR/Cas9 gene editing technology.
READ MORE

The State of Knock-ins in Zebrafish

By:
Alexandra Narin ,
The recent advancements of CRISPR and next-generation technology has enabled researchers to create more precise zebrafish models of human disease. This being said, knock-in (KI)
READ MORE

RedEfish: Generation Of The Polycistronic MScarlet: GSG-T2A: Ttpa Zebrafish Line

By:
Alexandra Narin ,
READ MORE

A Spotlight on Recent Zebrafish Research

By:
Ben Jussila ,
We’ve compiled some of the most recent informative and influential research being done using the zebrafish model
READ MORE

How do I get rid of this pesky protein? The Hows and Whys of Protein Degradation

By:
Trisha Brock ,
Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.
READ MORE

How to identify a successful transgenic edit in C. elegans Part 1

By:
Lauren Resch ,
In this blog, we will discuss key factors to consider when choosing a screening method, introduce various editing techniques, and discuss the pros & cons
READ MORE

90 Mutations with 1 Gene: Functional Analysis of Variants Using C. elegans

By:
Trisha Brock ,
Kathryn McCormick ,
We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants
READ MORE

PCR Vs. HRMA For Genotyping: When To Use What?

By:
Preston Kendrick ,
READ MORE

Fluorescent Tagging Capability Demonstration

By:
Lauren Resch ,
At InVivo Biosystems, we have been working with fluorescent tags in C. elegans for over five years, and we have made over 600 fluorescent transgenic lines.
READ MORE

We Used CRISPR To Make 15 Point Mutations In 5 Genes. Here’s What We Learned.

By:
Trisha Brock ,
Kathryn McCormick ,
READ MORE

Zebrafish Injection Mix: Buy verses DIY, what are the real costs? [Infographic]

By:
InVivo Biosystems ,
Make sure you understand all of the costs associated with DIY-ing a zebrafish CRISPR project verses outsourcing it to a team with years of experience.
READ MORE

Precise Knock-Outs For Precise Research

By:
Lauren Resch ,
READ MORE

11 Steps to a Successful CRISPR Knock-in

By:
Trisha Brock ,
Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in
READ MORE

6 Key Steps to a Successful Zebrafish Knock-in [Infographic]

By:
Hannah Huston ,
READ MORE

The Challenge of Getting CRISPR-based Knock-ins to Work in Zebrafish

By:
Chris Hopkins ,
Kathryn McCormick ,
We take a look into why so many zebrafish groups struggle to achieve knock-in transgenesis and what makes knock-ins so difficult.
READ MORE

6 Things That Can Go Wrong While Making Your Zebrafish CRISPR Knock-In

By:
Ben Jussila ,
READ MORE

To truly validate your sgRNAs, go In Vivo

By:
Marnie Preston ,
CRISPR/Cas9 gene editing for Zebrafish is a rapidly growing molecular biology toolset with diverse applications, but is not without its technological barriers. Reagent sourcing, design
READ MORE

Zebrafish modeling for the clinic: Rapid in vivo functional testing of patient variants for clinical applications.

By:
InVivo Biosystems ,
We use the zebrafish (Danio rerio) as an in vivo model to measure the functional effects of patient-derived genetic variation.  In this way, human genetic variants identified
READ MORE

Intro to CRISPR: Zebrafish Mutations [Infographic]

By:
Hannah Huston ,
READ MORE

CRISPR technologies enable humanized animal models to aid disease research

By:
InVivo Biosystems ,
C. elegans as a model to evaluate the function of disease genes In 1998, the soil nematode Caenorhabditis elegans became the first multicellular organism of
READ MORE

Arg…What’s up with that? Arginine is Enriched in Pathogenic Variants

By:
Chris Hopkins ,
Our CSO explores a theory he has about the amino acid arginine which seems to be showing frequent association with pathogenicity.
READ MORE

[Review] The Human Avatar

By:
Yoanne Clovis, Ph.D. ,
NemaMetrix is the market leader for early preclinical in-vivo testing using small animal models to gain a better understanding of the efficacy, mode of action,
READ MORE

Using Zebrafish for Rapid Genetic Analysis

By:
Hannah Huston ,
READ MORE

Article Review: The Caenorhabditis elegans Transgenic Toolbox

By:
Sophia Bethel ,
We reviewed a recent article that discusses the best techniques available for C. elegans transgenics, as well as experimental concerns that should be considered.
READ MORE

A Look Inside the CRISPR Toolbox

By:
Sophia Bethel ,
This visual quick-guide offers a look inside the control possible through CRISPR genome edited transgenics, and will offer guidance as to which method would be
READ MORE

Genomic baggage – What are the skeletons rattling in your genetic closet?

By:
Chris Hopkins ,
When you receive your genomic report, you have a movement of trepidation.What will it say? Will it have something that says you should do countermeasures
READ MORE

Conditional mutagenesis by loxP insertion in zebrafish – outfoxing the unfloxable

By:
Ben Jussila ,
CRISPR/Cas9-mediated integration of loxP sequences for conditional mutagenesis in zebrafish has recently made its way into the toolkit of zebrafish researchers. In particular, CRISPR/Cas9 mutagenesis
READ MORE

The Humanized Worm [Infographic]

By:
Anna Malinkevich, MS ,
Humanized model organisms are powerful biological models that allow scientists to study human disease genes in simpler contexts without losing the value of in vivo
READ MORE

New Milestone! We can now remove over 43,000 bp from C. elegans genomic DNA!

By:
Yoanne Clovis, Ph.D. ,
12k, 24k, 36k…we can now precisely remove over 43k bp from C. elegans genomic DNA! Knocking out genes is a commonly used tool for biologists
READ MORE

Dealing with Uncertain Significance of the Genome

By:
Chris Hopkins ,
“Risky Business” Most of us are wired to be risk averse. Yet, I have been giving serious contemplation to the “risky business” of having my whole
READ MORE

C. elegans as Fast and Affordable System for Variant Phenotyping

By:
Chris Hopkins ,
Systems for Functional Studies A variety of modeling systems can be used to explore variant function. Initially, many researchers turn to a computational approach to
READ MORE

Worming into Relevance – Human disease models in the C. elegans nematode

By:
Chris Hopkins ,
The philosopher Friedrich Nietzsche once said: “You have evolved from worm to man, but much within you is still worm.” Genetic diversity in individuals and
READ MORE
KCNQ2 epilepsy gene

Functional analysis of variants to aid understanding of children’s epilepsy

By:
InVivo Biosystems ,
Performing sequence analysis on a patient with a suspected genetic disease is becoming a standard of care. However, when a genetic variation is identified as
READ MORE

Worming into Relevance – Human disease models in the C. elegans nematode

By:
Chris Hopkins ,
The philosopher Friedrich Nietzsche once said: “You have evolved from worm to man, but much within you is still worm.” Genetic diversity in individuals and
READ MORE

CRISPR/Cas9 off target effects: Hyperactive cutting or media hype?

By:
Ben Jussila ,
There has been a lot of media coverage in recent months surrounding CRISPR/Cas9 and the potentially life changing impact the gene-editing technology could have. More
READ MORE

Insertion of two loxP sites to produce a knockout (KO) of an embryonic lethal gene

By:
Trisha Brock ,
A client wanted a knockout (KO) of an embryonic lethal gene. We could not make this line using our standard methods. Instead, we inserted two
READ MORE

An Abnormal Absence of Antibodies: Filling the Gaps with Gene Editing Tools in Zebrafish

By:
Ben Jussila ,
The zebrafish is an increasingly popular laboratory organism with many experimental advantages. The animal model has been used by biologists to discover the detailed processes
READ MORE

Genome wide associations and the nematode pharynx: ever the twain shall meet

By:
Shawn Lockery, Ph.D., Co-founder ,
Question: What might you get if you cross the feeding organ of a roundworm with a genome wide association study? Answer: A cornucopia of novel
READ MORE
Dr. Andy Golden

Unlocking the Potential of C. elegans for the Study of Rare Disease

By:
InVivo Biosystems ,
Dr. Andy Golden at NIH discusses his work on identifying regulators and interactors of conserved genes in C. elegans that, when mutated, can cause human disease
READ MORE

Modeling Rare Diseases in Caenorhabditis elegans

By:
,
There are approximately 7,000 rare diseases in humans, ~80% of which are monogenic. A rare disease is defined as affecting less than one in 1,500
READ MORE
The cerebellum of a brain affected by Niemann-Pick Type C; blue staining shows the dense pockets of lipid accumulations throughout the brain

Searching for a cure for Niemann-Pick Type C using C. elegans

By:
Terra Hiebert, Ph.D. ,
Yoanne Clovis, Ph.D. ,
Niemann-Pick Type C is a rare and fatal disorder affecting children. We explore the efficacy of new potential therapeutics with the ScreenChip System.
READ MORE
Load More
  • Categories

    • Connect with us

    Facebook Twitter Linkedin

    stay updated..

    The InVivo Biosystems Newsletter is delivered to your inbox bi-weekly,
    giving you all of the latest company updates, industry news, and more.

    Sign Up

    An expert in CRISPR genome editing, InVivo Biosystems creates custom genome-edited C. elegans and zebrafish models to enable aging, developmental, and disease studies. Our unique in vivo platforms and technologies bridge the gap between cells and mice.

    Quick Links

    Contact Us

    Headquarters: 1505 Westec Drive, Eugene, OR 97402

    1.844.663.8749.

    Facebook Twitter Linkedin

    Copyright © 2022 InVivo Biosystems. All rights reserved.

    Facebook Twitter Linkedin