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Gene Editing

Differences Between HDR vs NHEJ

Summary The CRISPR-Cas9 system has become integral in the toolbelts of genomic scientists, researchers, students, etc. Never has it been so easy to wield a pair of molecular scissors in your (pipetting) hand, however, there are a few intracellular processes to consider before cutting up your genome of interest. In this article, we will give …

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state of knock-ins in zebrafish

The State of Knock-ins in Zebrafish

The recent advancements of CRISPR and next-generation technology has enabled researchers to create more precise zebrafish models of human disease. This being said, knock-in (KI) techniques in zebrafish still aren’t fully optimized. In this article we discuss the current state of CRISPR-Cas9-mediated targeted knock-ins in zebrafish, and what the future holds.

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RedEfish: Generation of the Polycistronic mScarlet: GSG-T2A: Ttpa Zebrafish Line

RedEfish: Generation of the Polycistronic mScarlet: GSG-T2A: Ttpa Zebrafish Line

Dr. Robyn Tanguay is a distinguished professor at Oregon State University, and a collaborator with InVivio Biosystems. Dr. Tangauy utilizes zebrafish to study toxicology, and last month received a $7 million grant from the National Institute of Environmental Health Sciences to study the biological impacts of chemicals. In a recent publication, Dr. Tanguay and her lab created a new fluorescent-tagged zebrafish line to study the effects of Vitamin E on embryonic development. This article will give an overview of the Tanguay Lab’s work, and discuss: ‘why zebrafish’?

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