Category: Gene Editing

June 23, 2023

As personalized medicine and the discovery of de novo variants that cause disease continue to grow, researchers are increasingly in need of alternative models

4 May

A humanized Caenorhabditis elegans model of Hereditary Spastic Paraplegia [Where are they now: Customer Story]

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May 4, 2023

The zebrafish has become an increasingly prevalent model: but in this series we are going to dive deeper, discussing their unique benefits.

15 December

Whole-gene Humanized Animal Models (WHAM) with CRISPR – Re-imagining how Rare Disease therapeutics are developed

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December 15, 2022

Traditional modeling of patient variants in vivo has two limitations which can be overcome utilizing InVivo Biosystems’ patented WHAM-method.

23 November

America is finally investing in Biotechnology & Biomanufacturing in a Big Way

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November 23, 2022

In this article we discuss what President Biden’s New Executive Order means for America’s place in the biotech and biomanufacturing fields.

27 October

Looking at Gene Paralogs across both zebrafish and C. elegans Model Organisms [Customer Story]

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October 27, 2022

Drs Pruyne & Amack leveraged InVivo Biosystems’ Custom Injection Mix to design and create a novel zebrafish line.

25 August

Review: How a Small Worm is Helping Uncover the Pathogenicity of Gene Variants

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August 25, 2022

19 April

Differences Between HDR vs NHEJ

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Chesley Johnson ,

April 19, 2022

Summary The CRISPR-Cas9 system has become integral in the toolbelts of genomic scientists, researchers, students, etc. Never has it been so easy to wield a

24 March

How Do Knockins Work?

By:

Karl Stuntzner-Gibson ,

March 24, 2022

22 March

The ABCs of Genetic Research

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March 22, 2022

Here at InVivo Biosystems we spend a lot of time thinking, and talking, about genome editing. We thought it would be fun to compile a

8 March

Does everyone have digestive issues? — Gut dysbiosis is on the rise.

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March 8, 2022

In this article we discuss the rise in gut dysbiosis, and new risk factors: microplastics, consumption of supplements, and chronic stress.

3 March

How does Cre/loxP recombination work?

By:

Ashwin Suresh ,

Jenn Lawson ,

March 3, 2022

In this blog, we discuss the basics of the Cre/Lox technique which is used in conditionally controlling gene manipulation in zebrafish.

20 January

What is Zebrafish Knock-in Tagging?

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January 20, 2022

With knock-in tagging, you can tag your favorite gene at the native locus in your zebrafish line via CRISPR/Cas9 gene editing technology.

11 October

The State of Knock-ins in Zebrafish

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October 11, 2021

The recent advancements of CRISPR and next-generation technology has enabled researchers to create more precise zebrafish models of human disease. This being said, knock-in (KI)

23 September

RedEfish: Generation Of The Polycistronic MScarlet: GSG-T2A: Ttpa Zebrafish Line

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September 23, 2021

3 November

A Spotlight on Recent Zebrafish Research

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November 3, 2020

We’ve compiled some of the most recent informative and influential research being done using the zebrafish model

21 October

How do I get rid of this pesky protein? The Hows and Whys of Protein Degradation

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October 21, 2020

Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.

20 October

How to identify a successful transgenic edit in C. elegans Part 1

By:

Lauren Resch ,

October 20, 2020

In this blog, we will discuss key factors to consider when choosing a screening method, introduce various editing techniques, and discuss the pros & cons

25 September

90 Mutations with 1 Gene: Functional Analysis of Variants Using C. elegans

By:

Kathryn McCormick ,

September 25, 2020

We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants

7 August

PCR Vs. HRMA For Genotyping: When To Use What?

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Preston Kendrick ,

August 7, 2020

25 June

Fluorescent Tagging Capability Demonstration

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Lauren Resch ,

June 25, 2020

At InVivo Biosystems, we have been working with fluorescent tags in C. elegans for over five years, and we have made over 600 fluorescent transgenic lines.

18 June

We Used CRISPR To Make 15 Point Mutations In 5 Genes. Here’s What We Learned.

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Kathryn McCormick ,

June 18, 2020

17 June

Zebrafish Injection Mix: Buy verses DIY, what are the real costs? [Infographic]

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June 17, 2020

Make sure you understand all of the costs associated with DIY-ing a zebrafish CRISPR project verses outsourcing it to a team with years of experience.

1 June

Precise Knock-Outs For Precise Research

By:

Lauren Resch ,

June 1, 2020

30 April

11 Steps to a Successful CRISPR Knock-in

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April 30, 2020

Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in

22 April

6 Key Steps to a Successful Zebrafish Knock-in [Infographic]

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Hannah Huston ,

April 22, 2020

8 April

The Challenge of Getting CRISPR-based Knock-ins to Work in Zebrafish

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Kathryn McCormick ,

April 8, 2020

We take a look into why so many zebrafish groups struggle to achieve knock-in transgenesis and what makes knock-ins so difficult.

1 April

6 Things That Can Go Wrong While Making Your Zebrafish CRISPR Knock-In

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April 1, 2020

24 March

To truly validate your sgRNAs, go In Vivo

By:

Marnie Preston ,

March 24, 2020

CRISPR/Cas9 gene editing for Zebrafish is a rapidly growing molecular biology toolset with diverse applications, but is not without its technological barriers. Reagent sourcing, design

13 January

Zebrafish modeling for the clinic: Rapid in vivo functional testing of patient variants for clinical applications.

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January 13, 2020

We use the zebrafish (Danio rerio) as an in vivo model to measure the functional effects of patient-derived genetic variation. In this way, human genetic variants identified

4 December

Intro to CRISPR: Zebrafish Mutations [Infographic]

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Hannah Huston ,

December 4, 2019

14 November

CRISPR technologies enable humanized animal models to aid disease research

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November 14, 2019

C. elegans as a model to evaluate the function of disease genes In 1998, the soil nematode Caenorhabditis elegans became the first multicellular organism of

13 November

Arg…What’s up with that? Arginine is Enriched in Pathogenic Variants

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November 13, 2019

Our CSO explores a theory he has about the amino acid arginine which seems to be showing frequent association with pathogenicity.

18 October

[Review] The Human Avatar

By:

Yoanne Clovis, Ph.D. ,

October 18, 2019

NemaMetrix is the market leader for early preclinical in-vivo testing using small animal models to gain a better understanding of the efficacy, mode of action,

13 September

Using Zebrafish for Rapid Genetic Analysis

By:

Hannah Huston ,

September 13, 2019

11 September

Article Review: The Caenorhabditis elegans Transgenic Toolbox

By:

Sophia Bethel ,

September 11, 2019

We reviewed a recent article that discusses the best techniques available for C. elegans transgenics, as well as experimental concerns that should be considered.

4 September

A Look Inside the CRISPR Toolbox

By:

Sophia Bethel ,

September 4, 2019

This visual quick-guide offers a look inside the control possible through CRISPR genome edited transgenics, and will offer guidance as to which method would be

25 July

Genomic baggage – What are the skeletons rattling in your genetic closet?

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July 25, 2019

When you receive your genomic report, you have a movement of trepidation.What will it say? Will it have something that says you should do countermeasures

6 May

Conditional mutagenesis by loxP insertion in zebrafish – outfoxing the unfloxable

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May 6, 2019

CRISPR/Cas9-mediated integration of loxP sequences for conditional mutagenesis in zebrafish has recently made its way into the toolkit of zebrafish researchers. In particular, CRISPR/Cas9 mutagenesis

12 April

The Humanized Worm [Infographic]

By:

Anna Malinkevich, MS ,

April 12, 2019

Humanized model organisms are powerful biological models that allow scientists to study human disease genes in simpler contexts without losing the value of in vivo

25 February

New Milestone! We can now remove over 43,000 bp from C. elegans genomic DNA!

By:

Yoanne Clovis, Ph.D. ,

February 25, 2019

12k, 24k, 36k…we can now precisely remove over 43k bp from C. elegans genomic DNA! Knocking out genes is a commonly used tool for biologists

13 December

Dealing with Uncertain Significance of the Genome

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December 13, 2018

“Risky Business” Most of us are wired to be risk averse. Yet, I have been giving serious contemplation to the “risky business” of having my whole

28 November

C. elegans as Fast and Affordable System for Variant Phenotyping

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November 28, 2018

Systems for Functional Studies A variety of modeling systems can be used to explore variant function. Initially, many researchers turn to a computational approach to

20 November

Worming into Relevance – Human disease models in the C. elegans nematode

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November 20, 2018

The philosopher Friedrich Nietzsche once said: “You have evolved from worm to man, but much within you is still worm.” Genetic diversity in individuals and

5 November

Functional analysis of variants to aid understanding of children’s epilepsy

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November 5, 2018

Performing sequence analysis on a patient with a suspected genetic disease is becoming a standard of care. However, when a genetic variation is identified as

31 October

Worming into Relevance – Human disease models in the C. elegans nematode

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October 31, 2018

The philosopher Friedrich Nietzsche once said: “You have evolved from worm to man, but much within you is still worm.” Genetic diversity in individuals and

17 October

CRISPR/Cas9 off target effects: Hyperactive cutting or media hype?

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October 17, 2018

There has been a lot of media coverage in recent months surrounding CRISPR/Cas9 and the potentially life changing impact the gene-editing technology could have. More

3 October

Insertion of two loxP sites to produce a knockout (KO) of an embryonic lethal gene

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October 3, 2018

A client wanted a knockout (KO) of an embryonic lethal gene. We could not make this line using our standard methods. Instead, we inserted two

19 September

An Abnormal Absence of Antibodies: Filling the Gaps with Gene Editing Tools in Zebrafish

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September 19, 2018

The zebrafish is an increasingly popular laboratory organism with many experimental advantages. The animal model has been used by biologists to discover the detailed processes

7 May

Genome wide associations and the nematode pharynx: ever the twain shall meet

By:

Shawn Lockery, Ph.D., Co-founder ,

May 7, 2018

Question: What might you get if you cross the feeding organ of a roundworm with a genome wide association study? Answer: A cornucopia of novel

2 December

Unlocking the Potential of C. elegans for the Study of Rare Disease

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