CRISPR/Cas9 gene editing has revolutionized the field of genome engineering. InVivo specializes in providing highly accurate and efficient zebrafish knockout solutions. With our state-of-the-art CRISPR/Cas9 technology, we ensure precise and reliable genome editing in zebrafish. Our experienced scientists will guide you through the entire process, from sgRNA design to the generation and validation of knockout zebrafish lines.
More Zebrafish Gene Edit Offerings
Gene knockouts are a commonly used tool for biologists to understand gene function. Examination of phenotypes when the gene is deleted can reveal insights into what role that gene plays in the organism.
Using CRISPR/Cas9 in zebrafish, we can disrupt integral regulatory domains of a gene or the coding sequence of a gene (KO null), or even remove the entire functional domian or the entire coding sequence depending on gene size (KO deletion).
If deletion of your gene is lethal, consider making a floxed gene.
Standard Injection Mix
Validated sgRNA Injection Mix
Fully Validated Injection Mix
Mosaic Clutch* (F0 injected embryos)
Full Build** (Sequence verified hetero zygous line)
KO Design
Injection Mix Assembly
Locus Evaluation & Sequencing Primers
in vivo sgRNA Testing
in vivo Editing Assessment & Screening Reagents
Expertly Injected Embryos
Germline Transmission Screening & Line Propagation
* This service is only available to clients in the United States.
** Full Build includes screening n=100 adults; standard husbandry charge applies.
# Includes evaluation of 2 sgRNAs
## Includes evaluation of 4 sgRNAs
Choose this option if you want us to pick your sgRNA site, have already tested your sgRNA in vivo, or want to make a CRISPR knockout. We send a custom designed injection mix and you develop your own preferred detection/screening protocol.
What’s Included
Choose this option if you want us to guarantee your injection mix is designed around a high efficiency sgRNA site using our rigorous in-house testing process.
What’s Included?
Choose this option if you want a CRISPR knockin with completely validated reagents to use in your lab and to find your gene edited line. This is the best Custom Injection Mix option for the novice zebrafish line builder.
What’s Included?
Choose this option if you want a CRISPR knockin with completely validated reagents to use in your lab and to find your gene edited line. This is a good option for the researcher who wants to outsource part of the workload in the development of a new line.
What’s Included?
Choose this option if you want us to deliver a stable sequence validated CRISPR knockin line.
What’s Included?
We create a mix using quality custom reagents from our validated suppliers. The sgRNAs are duplexed with the Cas9 protein.
The mix is created using concentrations of Cas9, sgRNA and donor homology that work well in our genome editing pipeline. The mix is provided as a stable dehydrated reagent.
Four injection mixes are provided that reconstitute to 5ul of mix each.
You can miss your edit if you don’t have a good assay to detect it.
We will design and test a robust assay to detect your edit, which is critical when you begin germline screening.
We will also provide PCR primers and protocols for an edit detection assay.
Ensure that you have the quality reagents you need for a successful genome edit. The efficiency of the sgRNA guided cutting can vary widely and is directly tied to the achievement of a successful genome edit.
Algorithms and experience help us choose the best candidate sgRNAs for your project, but the guided cutting efficiency of an sgRNA can be impacted by genome organization, for example, chromatin accessibility. The best way to determine the effectiveness of an sgRNA in a Zebrafish genome is to test it in vivo in the injected embryos.
We test your sgRNAs for their ability to guide Cas9 cutting. Our process also identifies SNPs and polymorphoisms in your locus that may impact homology directed repair. Embryos are injected with two different sgRNA/Cas9 duplexes and we determine the cutting efficiency for each sgRNA. If both sgRNAs fail to guide successful cutting, a third sgRNA is injected and tested.
Additional sgRNA testing can also be added with consultation.
Have total peace of mind by having us test your injection mix in our facility.
Our skilled injectionists will inject embryos with your mix and determine the somatic integration efficiency by PCR.
We will confirm the precise edit by sequencing to ensure that your edit is occurring correctly with no erroneous insertions.
Our team precisely injects the sgRNAs targeting the locus or region of interest, Cas9 protein and the donor homology template to generate F0 embryos.
Expertly Injected Embryos
F0 embryos are reared to adulthood and then screened for germline transmission using a combination of screening techniques to identify germline founders. Identified adults are propagated to ensure a sufficient number of animals to ship to your facility.
Having very high similarity in genetic makeup with humans, zebrafish has proved to be an important model for human genetics and disease. By using zebrafish knockout or knock-in models, genes can be manipulated to understand different disease pathways.
CRISPR/Cas9 Zebrafish Knockout Service is a specialized scientific service that utilizes the CRISPR/Cas9 gene editing technology to create targeted gene knockouts in zebrafish embryos. It enables researchers to precisely disrupt specific genes of interest, providing a valuable tool for studying gene function and understanding the role of genes in various biological processes.
CRISPR/Cas9 Zebrafish Knockout Service works by designing and introducing specific guide RNAs (gRNAs) and Cas9 protein into zebrafish embryos. The gRNAs guide the Cas9 protein to the targeted gene, where it induces double-stranded breaks. These breaks are then repaired by the cell’s natural DNA repair mechanisms, often leading to the introduction of insertions or deletions (indels) that disrupt the function of the targeted gene.
CRISPR/Cas9 Zebrafish Knockout Service offers several advantages. It allows for precise and efficient gene knockout, providing researchers with a valuable tool to study gene function and model human diseases. Zebrafish embryos are transparent, rapidly develop, and share genetic similarities with humans, making them an ideal model organism for genetic research. The service saves time and resources compared to traditional knockout methods and enables researchers to generate multiple knockout lines simultaneously.
Yes, specific genes can be targeted with CRISPR/Cas9 Zebrafish Knockout Service. The design of the gRNAs determines the target gene. Researchers can choose to target any gene of interest by designing gRNAs that are complementary to the desired genomic sequence. This flexibility allows for precise and selective gene knockout.
The timeline for obtaining zebrafish knockout lines using CRISPR/Cas9 Zebrafish Knockout Service can vary depending on various factors, including the complexity of the targeted gene and the efficiency of the gene editing process. Typically, it takes a few weeks to generate founder fish with the desired genetic modifications. The subsequent steps, such as breeding and genotyping, may take several months to establish stable knockout lines.
Contact us so can provide a more accurate timeline based on the specific requirements of the project.
Ready to get started?
Ready to connect with us to learn more about working with our company or our technology?
Submit your inquiry below & we will get back to you soon.
The InVivo Biosystems Newsletter is delivered to your inbox bi-weekly,
giving you all of the latest company updates, industry news, and more.
Built on a robust portfolio of CRISPR genome editing patents, InVivo Biosystems has established a comprehensive preclinical discovery and development platform. By leveraging alternative models and proprietary methods, we accelerate go-to-market timelines for pharmaceutical companies and beyond.
