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Our Technologies

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Core Capabilities

InVivo Biosystems was founded on deep CRISPR genome editing expertise. This enabled us to create custom genome-edited zebrafish and C. elegans.

In the years since, we have expanded our offerings to include preclinical studies and drug discovery studies. Now, our CRISPR genome editing platform can provide key insights which can de-risk your clinical trial, and/or decide the next phase of your product development.

Our core capabilities allow us to provide services including:

  • in vivo model creation
  • toxicity studies
  • phenotype screening
  • target identification & validation
  • lead optimization

Genome Editing

Building accurate genetically editing models using advanced CRISPR and other genetic engineering tools.

CRISPR Knockout

CRISPR Knockout

Create deletion mutant of select gene.

CRISPR Knock-in

CRISPR Knock-in

Large cargo integrations.

CRISPR Point Mutation

CRISPR Point Mutation

Point mutation insertioint.

 

CRISPR Tag Knock-ins

CRISPR Tag Knock-ins

Insert tags (GFP, FLAG, HA, etc)

 

CRISPR Floxed Alleles

CRISPR Floxed Alleles

Insert loxP sites for tissue specific and temporal knockout

MosSCI Transgene Insertion

MosSCI Transgene Insertion

 Insert a large gene (ex. humanization) in C. elegans genome.

Transient Effect Crispants

Transient Effect Crispants

Evaluate gene function with F0 knockouts.

Deep Phenotyping

Providing multidimensional data on behavior and morphology using automated phenotyping platforms and machine learning.

High Throughput Screening

High Throughput Screening

Automated analysis using sensitive behavioral, morphological, and fluorescent assays.

 

Lifespan Study

Lifespan Study

Automated measurement of biometric parameters for long periods of time. 

Stress Response

Stress Response

Measuring oxidative stress, antioxidant function, heat-shock, osmotic stress, hypoxia and heavy metal toxicity in C. elegans

Behavior &  Morphology Analysis 

Behavior &  Morphology Analysis 

Zebrafish and C. elegans behavior and morphology analysis using the state of the art instrumentations. 

Imaging Analysis 

Imaging Analysis 

High resolution confocal, fluorescent imaging and Immunofluoresce imaging for antibody staining. 

Omics-Based Analysis

Examining the complete genetic and molecular profiles for determination of mechanism of action (MoA).

Transcriptomics

Transcriptomics

Complete set of RNA transcripts that are produced by the genome, under specific circumstances or in a specific tissue

Proteomics

Proteomics

Systematic, large-scale analysis of cellular proteins 

Metabolomics 

Metabolomics

Comprehensive analysis of small molecule substrates, intermediates, and products of cell metabolism

Genomics

Genomics

Whole genome sequencing.

Humanization

Replacing the native animal gene with the heterologous human gene for assessing function of an expressed human protein.

Whole Gene Humanization 

Whole Gene Humanization 

Human coding sequence insertion as substution for animals ortholog version of the gene.

Codon and Splice Optimization

Codon and Splice Optimization

Optimized codons, and artifical intron sequences between exon coding sequences of the heterologous gene to optimize expression levels.

 

VUS Calling

VUS calling

Point mutations within the gene to determine pathogenicity of variants of uncertain significance

 

Point Mutation

Point Mutation

Base substution or small indel to convert a highly homologous region of a zebrafish gene to match the human sequence.

Expression Analysis

Expression Analysis

Transcriptional and proteomic analysis to optimize expression levels to match human.

Lifetime Vitality Measurement

Determining a compound's effect on maintaining healthy aging by monitoring an animal throughout its lifespan.

Kaplan Meier Survival Curve

Kaplan Meier Survival Curve

Quantification of the survival of a population through time (aka “lifespan”).

 

Healthspan Measurement

Healthspan Measurement

Measurement of key health features including activity and morphology of a population through time.

 

Antioxidant Capacity Study

Antioxidant Capacity Study

Standardized reactive oxygen species exposure assay to uncover whether a compound or formulation has antioxidant properties.

 

Mitochondrial Function Study

Mitochondrial Function Study

Fluorescent imaging of mitochondrial structures to investigate the impact of a compound or formulation on mitochondrial function across the lifespan.

Protein Aggregation Study

Protein Aggregation Study

Fluorescent imaging and quantification of poly-Q protein aggregation throughout the lifespan. 

Sarcopenia Analysis

Sarcopenia Analysis

Measurement of animal activity during aging to investigate the impact of a compound or formulation of muscle function across the lifespan.

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