What is Zebrafish Knock-in Tagging?
With knock-in tagging, you can tag your favorite gene at the native locus in your zebrafish line via CRISPR/Cas9 gene editing technology.
CRISPR
The State of Knock-ins in Zebrafish
The recent advancements of CRISPR and next-generation technology has enabled researchers to create more precise zebrafish models of human disease. This being said, knock-in (KI) techniques in zebrafish still aren’t fully optimized. In this article we discuss the current state of CRISPR-Cas9-mediated targeted knock-ins in zebrafish, and what the future holds.
What’s a Knockout, Really?
The development of CRISPR-Cas9 editing has allowed researchers to quickly create precise gene knockouts (KO), but while CRISPR-Cas9 KOs have been highly publicized, are the different methods understood? In this article we will discuss the two available methods for creating knockout models using the CRISPR-Cas9 technology: their advantages and limitations, and how they are being used in research.
Creating Transgenic C. elegans Models For Clinical Variants of Unknown Significance [Customer Story]
Using the models provided by InVivo Biosystems, the Starr & Luxton Labs were able to focus their efforts on understanding and evaluating the variants and complete their project in a timely manner.
Let’s Fail Faster: Why Scientific Failures Should be Accepted as Progress
In the scientific community there is an emphasis on positive results: getting published, having a drug be approved, etc. However, crucial learning happens when experiments don’t work – in fact, these “failures” may be some of the most important learning experiences. In this article we discuss the need to shift the focus from failing less, to failing faster and some of the resources available to researchers so they can expedite their own work.
Validating Expression of Humanizations – What We’ve Done so Far
In an effort to create better research models for epilepsy, we’ve been working on a grant-funded project to humanize numerous C. elegans genes. Learn more about our progress and our findings in this blog.
So What is Humanization?
Humanized animal models are becoming more widely used as new gene editing techniques become available and there is a push for a more personalized approach to medicine, but what exactly is humanization? In this article we discuss the history, the types of humanized models, and how they can be applied to your research.
Problem Solved: Using a new technique to solve a problem
Two main lessons learned: 1. stay current with a broad variety of literature and 2. always be willing to try new methods
Check it before you wreck it
Mistakes can happen, various design issues can be overlooked, or maybe just a less than optimal design was used, so how do we at InVivo Biosystems minimize such events?
The Next Generation of Animal Models: Humanized Models
New ‘humanized models’ have the potential to revolutionize the study of clinical diseases, creating models with higher validity than more traditional models. This in turn has the power to expedite getting results from the bench to the bedside. In this article we will discuss the current state of animal models and the future that humanized models make possible; as an example, we will specifically focus on the models of Alzheimer’s Disease (AD).