Zebrafish Genome Editing: CRISPR vs. Tol2
One of our scientists compares two popular genome editing tools, CRISPR and Tol2 to better understand the pros and cons of each technology
CRISPR
A Spotlight on Recent Zebrafish Research
We’ve compiled some of the most recent informative and influential research being done using the zebrafish model
How do I get rid of this pesky protein? The Hows and Whys of Protein Degradation
Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.
90 Mutations with 1 Gene: Functional Analysis of Variants Using C. elegans
We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants were selected from the ClinVar database, literature, the Gnomad database, clinical researchers, and the STXBP1 foundation.
11 Steps to a Successful CRISPR Knock-in
Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in zebrafish so we want to share with you what we believe to be the 11 steps necessary to have a successful knock-in project.
The Challenge of Getting CRISPR-based Knock-ins to Work in Zebrafish
Just the other day, I was talking with a collaborator who told me that they didn’t really believe that knock-ins really worked in zebrafish. Although there have been literature reports for years, the number of labs that struggle and fail with this technique far outweigh the number that find success and publish. So I can …
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6 things that can go wrong while making your Zebrafish CRISPR Knock-In
With the advent of targeted nucleases like zinc-finger nucleases (ZFNs), TALE nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas), zebrafish researchers were able to begin interrogating gene function and regions of interest with a precision that was previously out of reach. With these innovations, researchers could not only disrupt …
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Zebrafish modeling for the clinic: Rapid in vivo functional testing of patient variants for clinical applications.
We use the zebrafish (Danio rerio) as an in vivo model to measure the functional effects of patient-derived genetic variation. In this way, human genetic variants identified in the clinic are quantitatively and qualitatively connected to model animal phenotypes. As a proof of concept study, we used precision gene editing and transient knockdown approaches targeting stxbp1a – the …
Intro to CRISPR: Zebrafish Mutations [Infographic]
Check out this infographic to learn more about the CRISPR mechanisms, zebrafish injections, and the different types of mutations available with zebrafish
CRISPR technologies enable humanized animal models to aid disease research
C. elegans as a model to evaluate the function of disease genes In 1998, the soil nematode Caenorhabditis elegans became the first multicellular organism of which the genome has been sequenced completely [1]. One surprising result of this approach was that ~65% of the human disease genes have a counterpart in the worm [2]. In …
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