Category: CRISPR

May 4, 2023

The zebrafish has become an increasingly prevalent model: but in this series we are going to dive deeper, discussing their unique benefits.

29 July

History of CRISPR: Cas9 and Cas12

By:

Yoanne Clovis, Ph.D. ,

July 29, 2022

6 July

What are Knock-outs and Knock-ins?

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July 6, 2022

19 April

Differences Between HDR vs NHEJ

By:

Chesley Johnson ,

April 19, 2022

Summary The CRISPR-Cas9 system has become integral in the toolbelts of genomic scientists, researchers, students, etc. Never has it been so easy to wield a

24 March

How Do Knockins Work?

By:

Karl Stuntzner-Gibson ,

March 24, 2022

22 March

The ABCs of Genetic Research

By:

March 22, 2022

Here at InVivo Biosystems we spend a lot of time thinking, and talking, about genome editing. We thought it would be fun to compile a

15 March

Zebrafish 101: A White Paper

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March 15, 2022

In this white paper we will discuss the advantages and limitations of zebrafish and ask: is a zebrafish model right for my research?

9 March

C. elegans 101: A White Paper

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March 9, 2022

In this whitepaper we discuss the advantages and limitations of C. elegans, and ask the question: is a C. elegans model right for my research?

18 February

Nutraceuticals & New approaches to DMD treatment

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February 18, 2022

This article will discuss recent findings, and how similar studies present an exciting and promising future for currently incurable diseases.

1 February

Neuroscience Models: Transgenic Mice, Zebrafish, C. Elegans – And Jellyfish?

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February 1, 2022

20 January

What is Zebrafish Knock-in Tagging?

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January 20, 2022

With knock-in tagging, you can tag your favorite gene at the native locus in your zebrafish line via CRISPR/Cas9 gene editing technology.

11 October

The State of Knock-ins in Zebrafish

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October 11, 2021

The recent advancements of CRISPR and next-generation technology has enabled researchers to create more precise zebrafish models of human disease. This being said, knock-in (KI)

6 August

What’s a Knockout, Really?

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August 6, 2021

The development of CRISPR-Cas9 editing has allowed researchers to quickly create precise gene knockouts (KO), but while CRISPR-Cas9 KOs have been highly publicized, are the

2 August

Creating Transgenic C. elegans Models For Clinical Variants of Unknown Significance [Customer Story]

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August 2, 2021

Using the models provided by InVivo Biosystems, the Starr & Luxton Labs were able to focus their efforts on understanding and evaluating the variants and

9 June

Let’s Fail Faster: Why Scientific Failures Should be Accepted as Progress

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June 9, 2021

In the scientific community there is an emphasis on positive results: getting published, having a drug be approved, etc. However, crucial learning happens when experiments

3 June

Validating Expression of Humanizations – What We’ve Done so Far

By:

Jenn Lawson ,

June 3, 2021

In an effort to create better research models for epilepsy, we’ve been working on a grant-funded project to humanize numerous C. elegans genes. Learn more

24 May

So What is Humanization?

By:

May 24, 2021

Humanized animal models are becoming more widely used as new gene editing techniques become available and there is a push for a more personalized approach

17 May

Problem Solved: Using a new technique to solve a problem

By:

Jenn Lawson ,

May 17, 2021

Two main lessons learned: 1. stay current with a broad variety of literature and 2. always be willing to try new methods

3 May

Check it before you wreck it

By:

Stelian Pop ,

May 3, 2021

Mistakes can happen, various design issues can be overlooked, or maybe just a less than optimal design was used, so how do we at InVivo

27 April

The Next Generation of Animal Models: Humanized Models

By:

April 27, 2021

New ‘humanized models’ have the potential to revolutionize the study of clinical diseases, creating models with higher validity than more traditional models. This in turn

22 March

Hey People ….its SSSH! time – Getting Easier Integration via Transgenesis Innovation

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March 22, 2021

Bottom line: Getting some SSSH! time with this split-hygR technique can calm the frustration of the aggravated C. elegans researcher.

9 March

Alzheimer’s – Have we been approaching it all wrong?

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March 9, 2021

A new finding has set the Alzheimer’s research world abuzz: a 73- year old woman with extremely high risk for AD, yet who doesn’t have

22 February

Whole Gene Humanization for Personalized Medicine Applications

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February 22, 2021

We have found whole gene humanization can create a platform for highly translatable results in a model organism. Currently there is a big need for

3 December

Zebrafish Genome Editing: CRISPR vs. Tol2

By:

Ashwin Suresh ,

December 3, 2020

One of our scientists compares two popular genome editing tools, CRISPR and Tol2 to better understand the pros and cons of each technology

3 November

A Spotlight on Recent Zebrafish Research

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November 3, 2020

We’ve compiled some of the most recent informative and influential research being done using the zebrafish model

21 October

How do I get rid of this pesky protein? The Hows and Whys of Protein Degradation

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October 21, 2020

Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.

25 September

90 Mutations with 1 Gene: Functional Analysis of Variants Using C. elegans

By:

Kathryn McCormick ,

September 25, 2020

We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants

30 April

11 Steps to a Successful CRISPR Knock-in

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April 30, 2020

Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in

8 April

The Challenge of Getting CRISPR-based Knock-ins to Work in Zebrafish

By:

Kathryn McCormick ,

April 8, 2020

We take a look into why so many zebrafish groups struggle to achieve knock-in transgenesis and what makes knock-ins so difficult.

1 April

6 Things That Can Go Wrong While Making Your Zebrafish CRISPR Knock-In

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April 1, 2020

13 January

Zebrafish modeling for the clinic: Rapid in vivo functional testing of patient variants for clinical applications.

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January 13, 2020

We use the zebrafish (Danio rerio) as an in vivo model to measure the functional effects of patient-derived genetic variation. In this way, human genetic variants identified

4 December

Intro to CRISPR: Zebrafish Mutations [Infographic]

By:

Hannah Huston ,

December 4, 2019

14 November

CRISPR technologies enable humanized animal models to aid disease research

By:

November 14, 2019

C. elegans as a model to evaluate the function of disease genes In 1998, the soil nematode Caenorhabditis elegans became the first multicellular organism of

11 September

Article Review: The Caenorhabditis elegans Transgenic Toolbox

By:

Sophia Bethel ,

September 11, 2019

We reviewed a recent article that discusses the best techniques available for C. elegans transgenics, as well as experimental concerns that should be considered.

4 September

A Look Inside the CRISPR Toolbox

By:

Sophia Bethel ,

September 4, 2019

This visual quick-guide offers a look inside the control possible through CRISPR genome edited transgenics, and will offer guidance as to which method would be

7 June

What Can Zebrafish Tell us About CRISPR Off-Target Activity?

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June 7, 2019

In 2017, a now-redacted paper published in Nature Methods claimed that CRISPR created hundreds of unintended mutations in mouse models, and that algorithms designed to

28 November

C. elegans as Fast and Affordable System for Variant Phenotyping

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November 28, 2018

Systems for Functional Studies A variety of modeling systems can be used to explore variant function. Initially, many researchers turn to a computational approach to

20 November

Worming into Relevance – Human disease models in the C. elegans nematode

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November 20, 2018

The philosopher Friedrich Nietzsche once said: “You have evolved from worm to man, but much within you is still worm.” Genetic diversity in individuals and

5 November

Functional analysis of variants to aid understanding of children’s epilepsy

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November 5, 2018

Performing sequence analysis on a patient with a suspected genetic disease is becoming a standard of care. However, when a genetic variation is identified as

31 October

Worming into Relevance – Human disease models in the C. elegans nematode

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October 31, 2018

The philosopher Friedrich Nietzsche once said: “You have evolved from worm to man, but much within you is still worm.” Genetic diversity in individuals and

17 October

CRISPR/Cas9 off target effects: Hyperactive cutting or media hype?

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