InVivo Biosystems Blog

Using Zebrafish to Combat Cognitive Decline: How a Biopharmaceutical Company Leveraged a Zebrafish Model to characterize a Compound for Neurodegenerative Disease Therapeutics

The zebrafish has become an increasingly prevalent model: but in this series we are going to dive deeper, discussing their unique benefits.
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A humanized Caenorhabditis elegans model of Hereditary Spastic Paraplegia [Where are they now: Customer Story]

The zebrafish has become an increasingly prevalent model: but in this series we are going to dive deeper, discussing their unique benefits.

History of CRISPR: Cas9 and Cas12

What are Knock-outs and Knock-ins?

Differences Between HDR vs NHEJ

Summary The CRISPR-Cas9 system has become integral in the toolbelts of genomic scientists, researchers, students, etc. Never has it been so easy to wield a

How Do Knockins Work?

The ABCs of Genetic Research

Here at InVivo Biosystems we spend a lot of time thinking, and talking, about genome editing. We thought it would be fun to compile a

Zebrafish 101: A White Paper

In this white paper we will discuss the advantages and limitations of zebrafish and ask: is a zebrafish model right for my research?

C. elegans 101: A White Paper

In this whitepaper we discuss the advantages and limitations of C. elegans, and ask the question: is a C. elegans model right for my research?
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Nutraceuticals & New approaches to DMD treatment

This article will discuss recent findings, and how similar studies present an exciting and promising future for currently incurable diseases.

Neuroscience Models: Transgenic Mice, Zebrafish, C. Elegans – And Jellyfish?

What is Zebrafish Knock-in Tagging?

With knock-in tagging, you can tag your favorite gene at the native locus in your zebrafish line via CRISPR/Cas9 gene editing technology.

The State of Knock-ins in Zebrafish

The recent advancements of CRISPR and next-generation technology has enabled researchers to create more precise zebrafish models of human disease. This being said, knock-in (KI)

What’s a Knockout, Really?

The development of CRISPR-Cas9 editing has allowed researchers to quickly create precise gene knockouts (KO), but while CRISPR-Cas9 KOs have been highly publicized, are the

Creating Transgenic C. elegans Models For Clinical Variants of Unknown Significance [Customer Story]

Using the models provided by InVivo Biosystems, the Starr & Luxton Labs were able to focus their efforts on understanding and evaluating the variants and

Let’s Fail Faster: Why Scientific Failures Should be Accepted as Progress

In the scientific community there is an emphasis on positive results: getting published, having a drug be approved, etc. However, crucial learning happens when experiments

Validating Expression of Humanizations – What We’ve Done so Far

In an effort to create better research models for epilepsy, we’ve been working on a grant-funded project to humanize numerous C. elegans genes. Learn more

So What is Humanization?

Humanized animal models are becoming more widely used as new gene editing techniques become available and there is a push for a more personalized approach

Problem Solved: Using a new technique to solve a problem

Two main lessons learned: 1. stay current with a broad variety of literature and 2. always be willing to try new methods

Check it before you wreck it

Mistakes can happen, various design issues can be overlooked, or maybe just a less than optimal design was used, so how do we at InVivo

The Next Generation of Animal Models: Humanized Models

New ‘humanized models’ have the potential to revolutionize the study of clinical diseases, creating models with higher validity than more traditional models. This in turn

Hey People ….its SSSH! time – Getting Easier Integration via Transgenesis Innovation

Bottom line: Getting some SSSH! time with this split-hygR technique can calm the frustration of the aggravated C. elegans researcher.

Alzheimer’s – Have we been approaching it all wrong?

A new finding has set the Alzheimer’s research world abuzz: a 73- year old woman with extremely high risk for AD, yet who doesn’t have

Whole Gene Humanization for Personalized Medicine Applications

We have found whole gene humanization can create a platform for highly translatable results in a model organism. Currently there is a big need for

Zebrafish Genome Editing: CRISPR vs. Tol2

One of our scientists compares two popular genome editing tools, CRISPR and Tol2 to better understand the pros and cons of each technology

A Spotlight on Recent Zebrafish Research

We’ve compiled some of the most recent informative and influential research being done using the zebrafish model

How do I get rid of this pesky protein? The Hows and Whys of Protein Degradation

Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.

90 Mutations with 1 Gene: Functional Analysis of Variants Using C. elegans

We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants

11 Steps to a Successful CRISPR Knock-in

Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in

The Challenge of Getting CRISPR-based Knock-ins to Work in Zebrafish

We take a look into why so many zebrafish groups struggle to achieve knock-in transgenesis and what makes knock-ins so difficult.

6 Things That Can Go Wrong While Making Your Zebrafish CRISPR Knock-In

Zebrafish modeling for the clinic: Rapid in vivo functional testing of patient variants for clinical applications.

We use the zebrafish (Danio rerio) as an in vivo model to measure the functional effects of patient-derived genetic variation.  In this way, human genetic variants identified

Intro to CRISPR: Zebrafish Mutations [Infographic]

CRISPR technologies enable humanized animal models to aid disease research

C. elegans as a model to evaluate the function of disease genes In 1998, the soil nematode Caenorhabditis elegans became the first multicellular organism of

Article Review: The Caenorhabditis elegans Transgenic Toolbox

We reviewed a recent article that discusses the best techniques available for C. elegans transgenics, as well as experimental concerns that should be considered.

A Look Inside the CRISPR Toolbox

This visual quick-guide offers a look inside the control possible through CRISPR genome edited transgenics, and will offer guidance as to which method would be

What Can Zebrafish Tell us About CRISPR Off-Target Activity?

In 2017, a now-redacted paper published in Nature Methods claimed that CRISPR created hundreds of unintended mutations in mouse models, and that algorithms designed to

C. elegans as Fast and Affordable System for Variant Phenotyping

Systems for Functional Studies A variety of modeling systems can be used to explore variant function. Initially, many researchers turn to a computational approach to

Worming into Relevance – Human disease models in the C. elegans nematode

The philosopher Friedrich Nietzsche once said: “You have evolved from worm to man, but much within you is still worm.” Genetic diversity in individuals and
KCNQ2 epilepsy gene

Functional analysis of variants to aid understanding of children’s epilepsy

Performing sequence analysis on a patient with a suspected genetic disease is becoming a standard of care. However, when a genetic variation is identified as

Worming into Relevance – Human disease models in the C. elegans nematode

The philosopher Friedrich Nietzsche once said: “You have evolved from worm to man, but much within you is still worm.” Genetic diversity in individuals and

CRISPR/Cas9 off target effects: Hyperactive cutting or media hype?

There has been a lot of media coverage in recent months surrounding CRISPR/Cas9 and the potentially life changing impact the gene-editing technology could have. More
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