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InVivo Biosystems Blog

Category: CRISPR

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A humanized Caenorhabditis elegans model of Hereditary Spastic Paraplegia [Where are they now: Customer Story]

By:
Alexandra Narin ,
The zebrafish has become an increasingly prevalent model: but in this series we are going to dive deeper, discussing their unique benefits.
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History of CRISPR: Cas9 and Cas12

By:
Yoanne Clovis, Ph.D. ,
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What are Knock-outs and Knock-ins?

By:
InVivo Biosystems ,
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Differences Between HDR vs NHEJ

By:
Chesley Johnson ,
Summary The CRISPR-Cas9 system has become integral in the toolbelts of genomic scientists, researchers, students, etc. Never has it been so easy to wield a
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How Do Knockins Work?

By:
Karl Stuntzner-Gibson ,
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The ABCs of Genetic Research

By:
Alexandra Narin ,
Here at InVivo Biosystems we spend a lot of time thinking, and talking, about genome editing. We thought it would be fun to compile a
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Zebrafish 101: A White Paper

By:
Alexandra Narin ,
In this white paper we will discuss the advantages and limitations of zebrafish and ask: is a zebrafish model right for my research?
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C. elegans 101: A White Paper

By:
Alexandra Narin ,
In this whitepaper we discuss the advantages and limitations of C. elegans, and ask the question: is a C. elegans model right for my research?
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Nutraceuticals & New approaches to DMD treatment

By:
Alexandra Narin ,
This article will discuss recent findings, and how similar studies present an exciting and promising future for currently incurable diseases.
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Neuroscience Models: Transgenic Mice, Zebrafish, C. Elegans – And Jellyfish?

By:
Alexandra Narin ,
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What is Zebrafish Knock-in Tagging?

By:
Ben Jussila ,
With knock-in tagging, you can tag your favorite gene at the native locus in your zebrafish line via CRISPR/Cas9 gene editing technology.
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The State of Knock-ins in Zebrafish

By:
Alexandra Narin ,
The recent advancements of CRISPR and next-generation technology has enabled researchers to create more precise zebrafish models of human disease. This being said, knock-in (KI)
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What’s a Knockout, Really?

By:
Trisha Brock ,
Alexandra Narin ,
The development of CRISPR-Cas9 editing has allowed researchers to quickly create precise gene knockouts (KO), but while CRISPR-Cas9 KOs have been highly publicized, are the
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Creating Transgenic C. elegans Models For Clinical Variants of Unknown Significance [Customer Story]

By:
Alexandra Narin ,
Using the models provided by InVivo Biosystems, the Starr & Luxton Labs were able to focus their efforts on understanding and evaluating the variants and
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Let’s Fail Faster: Why Scientific Failures Should be Accepted as Progress

By:
Alexandra Narin ,
In the scientific community there is an emphasis on positive results: getting published, having a drug be approved, etc. However, crucial learning happens when experiments
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Validating Expression of Humanizations – What We’ve Done so Far

By:
Jenn Lawson ,
In an effort to create better research models for epilepsy, we’ve been working on a grant-funded project to humanize numerous C. elegans genes. Learn more
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So What is Humanization?

By:
Alexandra Narin ,
Humanized animal models are becoming more widely used as new gene editing techniques become available and there is a push for a more personalized approach
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Problem Solved: Using a new technique to solve a problem

By:
Jenn Lawson ,
Two main lessons learned: 1. stay current with a broad variety of literature and 2. always be willing to try new methods
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Check it before you wreck it

By:
Stelian Pop ,
Mistakes can happen, various design issues can be overlooked, or maybe just a less than optimal design was used, so how do we at InVivo
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The Next Generation of Animal Models: Humanized Models

By:
Alexandra Narin ,
New ‘humanized models’ have the potential to revolutionize the study of clinical diseases, creating models with higher validity than more traditional models. This in turn
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Hey People ….its SSSH! time – Getting Easier Integration via Transgenesis Innovation

By:
Chris Hopkins ,
Bottom line: Getting some SSSH! time with this split-hygR technique can calm the frustration of the aggravated C. elegans researcher.
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Alzheimer’s – Have we been approaching it all wrong?

By:
Alexandra Narin ,
A new finding has set the Alzheimer’s research world abuzz: a 73- year old woman with extremely high risk for AD, yet who doesn’t have
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Whole Gene Humanization for Personalized Medicine Applications

By:
Chris Hopkins ,
We have found whole gene humanization can create a platform for highly translatable results in a model organism. Currently there is a big need for
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Zebrafish Genome Editing: CRISPR vs. Tol2

By:
Ashwin Suresh ,
One of our scientists compares two popular genome editing tools, CRISPR and Tol2 to better understand the pros and cons of each technology
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A Spotlight on Recent Zebrafish Research

By:
Ben Jussila ,
We’ve compiled some of the most recent informative and influential research being done using the zebrafish model
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How do I get rid of this pesky protein? The Hows and Whys of Protein Degradation

By:
Trisha Brock ,
Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.
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90 Mutations with 1 Gene: Functional Analysis of Variants Using C. elegans

By:
Trisha Brock ,
Kathryn McCormick ,
We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants
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11 Steps to a Successful CRISPR Knock-in

By:
Trisha Brock ,
Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in
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The Challenge of Getting CRISPR-based Knock-ins to Work in Zebrafish

By:
Chris Hopkins ,
Kathryn McCormick ,
We take a look into why so many zebrafish groups struggle to achieve knock-in transgenesis and what makes knock-ins so difficult.
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6 Things That Can Go Wrong While Making Your Zebrafish CRISPR Knock-In

By:
Ben Jussila ,
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Zebrafish modeling for the clinic: Rapid in vivo functional testing of patient variants for clinical applications.

By:
InVivo Biosystems ,
We use the zebrafish (Danio rerio) as an in vivo model to measure the functional effects of patient-derived genetic variation.  In this way, human genetic variants identified
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Intro to CRISPR: Zebrafish Mutations [Infographic]

By:
Hannah Huston ,
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CRISPR technologies enable humanized animal models to aid disease research

By:
InVivo Biosystems ,
C. elegans as a model to evaluate the function of disease genes In 1998, the soil nematode Caenorhabditis elegans became the first multicellular organism of
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Article Review: The Caenorhabditis elegans Transgenic Toolbox

By:
Sophia Bethel ,
We reviewed a recent article that discusses the best techniques available for C. elegans transgenics, as well as experimental concerns that should be considered.
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A Look Inside the CRISPR Toolbox

By:
Sophia Bethel ,
This visual quick-guide offers a look inside the control possible through CRISPR genome edited transgenics, and will offer guidance as to which method would be
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What Can Zebrafish Tell us About CRISPR Off-Target Activity?

By:
InVivo Biosystems ,
In 2017, a now-redacted paper published in Nature Methods claimed that CRISPR created hundreds of unintended mutations in mouse models, and that algorithms designed to
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C. elegans as Fast and Affordable System for Variant Phenotyping

By:
Chris Hopkins ,
Systems for Functional Studies A variety of modeling systems can be used to explore variant function. Initially, many researchers turn to a computational approach to
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Worming into Relevance – Human disease models in the C. elegans nematode

By:
Chris Hopkins ,
The philosopher Friedrich Nietzsche once said: “You have evolved from worm to man, but much within you is still worm.” Genetic diversity in individuals and
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KCNQ2 epilepsy gene

Functional analysis of variants to aid understanding of children’s epilepsy

By:
InVivo Biosystems ,
Performing sequence analysis on a patient with a suspected genetic disease is becoming a standard of care. However, when a genetic variation is identified as
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Worming into Relevance – Human disease models in the C. elegans nematode

By:
Chris Hopkins ,
The philosopher Friedrich Nietzsche once said: “You have evolved from worm to man, but much within you is still worm.” Genetic diversity in individuals and
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CRISPR/Cas9 off target effects: Hyperactive cutting or media hype?

By:
Ben Jussila ,
There has been a lot of media coverage in recent months surrounding CRISPR/Cas9 and the potentially life changing impact the gene-editing technology could have. More
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