fbpx
Contact Us

InVivo Biosystems Blog

Category: CRISPR

History of CRISPR: Cas9 and Cas12

By:
Yoanne Clovis, Ph.D. ,
READ MORE

What are Knock-outs and Knock-ins?

By:
InVivo Biosystems ,
READ MORE

Differences Between HDR vs NHEJ

By:
Chesley Johnson ,
Summary The CRISPR-Cas9 system has become integral in the toolbelts of genomic scientists, researchers, students, etc. Never has it been so easy to wield a
READ MORE

How Do Knockins Work?

By:
Karl Stuntzner-Gibson ,
READ MORE

The ABCs of Genetic Research

By:
Alexandra Narin ,
Here at InVivo Biosystems we spend a lot of time thinking, and talking, about genome editing. We thought it would be fun to compile a
READ MORE

Zebrafish 101: A White Paper

By:
Alexandra Narin ,
In this white paper we will discuss the advantages and limitations of zebrafish and ask: is a zebrafish model right for my research?
READ MORE

C. elegans 101: A White Paper

By:
Alexandra Narin ,
In this whitepaper we discuss the advantages and limitations of C. elegans, and ask the question: is a C. elegans model right for my research?
READ MORE
DMD feature image

Nutraceuticals & New approaches to DMD treatment

By:
Alexandra Narin ,
This article will discuss recent findings, and how similar studies present an exciting and promising future for currently incurable diseases.
READ MORE

Neuroscience Models: Transgenic Mice, Zebrafish, C. Elegans – And Jellyfish?

By:
Alexandra Narin ,
READ MORE

What is Zebrafish Knock-in Tagging?

By:
Ben Jussila ,
With knock-in tagging, you can tag your favorite gene at the native locus in your zebrafish line via CRISPR/Cas9 gene editing technology.
READ MORE

The State of Knock-ins in Zebrafish

By:
Alexandra Narin ,
The recent advancements of CRISPR and next-generation technology has enabled researchers to create more precise zebrafish models of human disease. This being said, knock-in (KI)
READ MORE

What’s a Knockout, Really?

By:
Trisha Brock ,
Alexandra Narin ,
The development of CRISPR-Cas9 editing has allowed researchers to quickly create precise gene knockouts (KO), but while CRISPR-Cas9 KOs have been highly publicized, are the
READ MORE

Creating Transgenic C. elegans Models For Clinical Variants of Unknown Significance [Customer Story]

By:
Alexandra Narin ,
Using the models provided by InVivo Biosystems, the Starr & Luxton Labs were able to focus their efforts on understanding and evaluating the variants and
READ MORE

Let’s Fail Faster: Why Scientific Failures Should be Accepted as Progress

By:
Alexandra Narin ,
In the scientific community there is an emphasis on positive results: getting published, having a drug be approved, etc. However, crucial learning happens when experiments
READ MORE

Validating Expression of Humanizations – What We’ve Done so Far

By:
Jenn Lawson ,
In an effort to create better research models for epilepsy, we’ve been working on a grant-funded project to humanize numerous C. elegans genes. Learn more
READ MORE

So What is Humanization?

By:
Alexandra Narin ,
Humanized animal models are becoming more widely used as new gene editing techniques become available and there is a push for a more personalized approach
READ MORE

Problem Solved: Using a new technique to solve a problem

By:
Jenn Lawson ,
Two main lessons learned: 1. stay current with a broad variety of literature and 2. always be willing to try new methods
READ MORE

Check it before you wreck it

By:
Stelian Pop ,
Mistakes can happen, various design issues can be overlooked, or maybe just a less than optimal design was used, so how do we at InVivo
READ MORE

The Next Generation of Animal Models: Humanized Models

By:
Alexandra Narin ,
New ‘humanized models’ have the potential to revolutionize the study of clinical diseases, creating models with higher validity than more traditional models. This in turn
READ MORE

Hey People ….its SSSH! time – Getting Easier Integration via Transgenesis Innovation

By:
Chris Hopkins ,
Bottom line: Getting some SSSH! time with this split-hygR technique can calm the frustration of the aggravated C. elegans researcher.
READ MORE

Alzheimer’s – Have we been approaching it all wrong?

By:
Alexandra Narin ,
A new finding has set the Alzheimer’s research world abuzz: a 73- year old woman with extremely high risk for AD, yet who doesn’t have
READ MORE

Whole Gene Humanization for Personalized Medicine Applications

By:
Chris Hopkins ,
We have found whole gene humanization can create a platform for highly translatable results in a model organism. Currently there is a big need for
READ MORE

Zebrafish Genome Editing: CRISPR vs. Tol2

By:
Ashwin Suresh ,
One of our scientists compares two popular genome editing tools, CRISPR and Tol2 to better understand the pros and cons of each technology
READ MORE

A Spotlight on Recent Zebrafish Research

By:
Ben Jussila ,
We’ve compiled some of the most recent informative and influential research being done using the zebrafish model
READ MORE

How do I get rid of this pesky protein? The Hows and Whys of Protein Degradation

By:
Trisha Brock ,
Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.
READ MORE

90 Mutations with 1 Gene: Functional Analysis of Variants Using C. elegans

By:
Trisha Brock ,
Kathryn McCormick ,
We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants
READ MORE

11 Steps to a Successful CRISPR Knock-in

By:
Trisha Brock ,
Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in
READ MORE

The Challenge of Getting CRISPR-based Knock-ins to Work in Zebrafish

By:
Chris Hopkins ,
Kathryn McCormick ,
We take a look into why so many zebrafish groups struggle to achieve knock-in transgenesis and what makes knock-ins so difficult.
READ MORE

6 Things That Can Go Wrong While Making Your Zebrafish CRISPR Knock-In

By:
Ben Jussila ,
READ MORE

Zebrafish modeling for the clinic: Rapid in vivo functional testing of patient variants for clinical applications.

By:
InVivo Biosystems ,
We use the zebrafish (Danio rerio) as an in vivo model to measure the functional effects of patient-derived genetic variation.  In this way, human genetic variants identified
READ MORE

Intro to CRISPR: Zebrafish Mutations [Infographic]

By:
Hannah Huston ,
READ MORE

CRISPR technologies enable humanized animal models to aid disease research

By:
InVivo Biosystems ,
C. elegans as a model to evaluate the function of disease genes In 1998, the soil nematode Caenorhabditis elegans became the first multicellular organism of
READ MORE

Article Review: The Caenorhabditis elegans Transgenic Toolbox

By:
Sophia Bethel ,
We reviewed a recent article that discusses the best techniques available for C. elegans transgenics, as well as experimental concerns that should be considered.
READ MORE

A Look Inside the CRISPR Toolbox

By:
Sophia Bethel ,
This visual quick-guide offers a look inside the control possible through CRISPR genome edited transgenics, and will offer guidance as to which method would be
READ MORE

What Can Zebrafish Tell us About CRISPR Off-Target Activity?

By:
InVivo Biosystems ,
In 2017, a now-redacted paper published in Nature Methods claimed that CRISPR created hundreds of unintended mutations in mouse models, and that algorithms designed to
READ MORE

C. elegans as Fast and Affordable System for Variant Phenotyping

By:
Chris Hopkins ,
Systems for Functional Studies A variety of modeling systems can be used to explore variant function. Initially, many researchers turn to a computational approach to
READ MORE

Worming into Relevance – Human disease models in the C. elegans nematode

By:
Chris Hopkins ,
The philosopher Friedrich Nietzsche once said: “You have evolved from worm to man, but much within you is still worm.” Genetic diversity in individuals and
READ MORE
KCNQ2 epilepsy gene

Functional analysis of variants to aid understanding of children’s epilepsy

By:
InVivo Biosystems ,
Performing sequence analysis on a patient with a suspected genetic disease is becoming a standard of care. However, when a genetic variation is identified as
READ MORE

Worming into Relevance – Human disease models in the C. elegans nematode

By:
Chris Hopkins ,
The philosopher Friedrich Nietzsche once said: “You have evolved from worm to man, but much within you is still worm.” Genetic diversity in individuals and
READ MORE

CRISPR/Cas9 off target effects: Hyperactive cutting or media hype?

By:
Ben Jussila ,
There has been a lot of media coverage in recent months surrounding CRISPR/Cas9 and the potentially life changing impact the gene-editing technology could have. More
READ MORE
Load More
  • Categories

    • Connect with us

    Facebook Twitter Linkedin

    stay updated..

    The InVivo Biosystems Newsletter is delivered to your inbox bi-weekly,
    giving you all of the latest company updates, industry news, and more.

    Sign Up

    An expert in CRISPR genome editing, InVivo Biosystems creates custom genome-edited C. elegans and zebrafish models to enable aging, developmental, and disease studies. Our unique in vivo platforms and technologies bridge the gap between cells and mice.

    Quick Links

    Contact Us

    Headquarters: 1505 Westec Drive, Eugene, OR 97402

    1.844.663.8749.

    Facebook Twitter Linkedin

    Copyright © 2022 InVivo Biosystems. All rights reserved.

    Facebook Twitter Linkedin