We have found whole gene humanization can create a platform for highly translatable results in a model organism. Currently there is a big need for functional studies of Variants of Uncertain Significance (VUS).
One of our scientists compares two popular genome editing tools, CRISPR and Tol2 to better understand the pros and cons of each technology
We’ve compiled some of the most recent informative and influential research being done using the zebrafish model
Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.
We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants were selected from the ClinVar database, literature, the Gnomad database, clinical researchers, and the STXBP1 foundation.
Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in zebrafish so we want to share with you what we believe to be the 11 steps necessary to have a successful knock-in project.
We take a look into why so many zebrafish groups struggle to achieve knock-in transgenesis and what makes knock-ins so difficult.
With the advent of targeted nucleases like zinc-finger nucleases (ZFNs), TALE nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas), zebrafish researchers were able to begin interrogating gene function and regions of interest with a precision that was previously out of reach. With these innovations, researchers could not only disrupt …
6 things that can go wrong while making your Zebrafish CRISPR Knock-In Read More »
We use the zebrafish (Danio rerio) as an in vivo model to measure the functional effects of patient-derived genetic variation. In this way, human genetic variants identified in the clinic are quantitatively and qualitatively connected to model animal phenotypes. As a proof of concept study, we used precision gene editing and transient knockdown approaches targeting stxbp1a – the …
Check out this infographic to learn more about the CRISPR mechanisms, zebrafish injections, and the different types of mutations available with zebrafish
