In Vivo Models
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Zebrafish as a research model has become increasingly popular in recent years due to their small size, transparency during development, and ability to reproduce quickly. While rodent or mice models have been used extensively for research purposes, zebrafish data can complement data from rodent or mice models by providing unique insights into early development, allowing for high-throughput screening, enabling genetic manipulation, facilitating comparative biology, and enabling behavioral studies.
By using both models, researchers can take advantage of the strengths of each model for a wide range of biomedical research applications. These complementary data sets can help researchers gain a more comprehensive understanding of biological processes and disease mechanisms, ultimately leading to the development of more effective treatments and therapies for human diseases.
Determine if a lead compound ameliorates disease phenotypes or affect important metabolites.
Determine if genes are involved in disease processes and make good targets for further discovery.
Medium throughput screen of compound libraries and biologics (up to BSL2).
Tissue preparation for ADME analysis. General and tissue specific toxicity
As an expert in zebrafish research, our core competencies reside in the following areas:
Our phenotype-based drug discovery process uses zebrafish to bridge the gap between cell culture and murine models. Using zebrafish as an in vivo model, drug discovery can be target independent and results based from the very start. This approach focuses on ameliorating phenotypes and allows efficacious drugs to be identified more quickly and readily, costing less time and money. And, because of advances in genetics allowing us to reliably express human proteins the zebrafish model is more translational than ever before. Our services are suitable for projects such as:
As a world leader in zebrafish research and services, we can provide end-to-end service including design and line generation, phenotypic data collection and interpretation using the zebrafish model. We provide the following services:
Therapeutics Testing & Evaluation
— Genetically modified —
— Knockout
— Knock-in
— Point mutation
— Humanized
— Chemical models —
— Small Molecule
— ASO
— mRNA
— Biologics
— Library Screening
— Phenotypic
— Molecular
— Protein Analysis
— Imaging
— Gene Expression
— Bioavailability
Genetic Target Validation & Discovery
– Crispant screening: We use CRISPR to generate transient KOs (termed F0 crispants) for a gene of interest. This can be used to rapidly determine whether genes are involved in disease processes and make good targets for further discovery.
—- Create knockouts in the first generation
—- Disrupt 10-100’s genes
— Phenotypic
— Molecular
— Protein Analysis
— Imaging
— Gene Expression
— Discover which genes are disease relevant
Library & Candidate Screening
– Screen compound libraries and biologics (up to BSL2) for effects on a variety of readouts, including gene expression, fluorescence, and morphometrics.
— Genetically modified —
— Knockout
— Knock-in
— Point mutation
— Humanized
— Chemical models —
— Targeted
— Repurposed drug library
— Phenotypic
— Molecular
— Protein Analysis
— Imaging
— Gene Expression
— Bioavailability
Acute Toxicity Testing
– Our Acute Embryo Toxicity testing meets OECD 236 guidelines. We also offer targeted hepatotoxicity, neurotoxicity, and cardiotoxicity analysis.
— Test a range of concentrations, we define concentrations such as the LD50, NOEL, and MNLD.ing.
— Assess Morphological endpoints and Behavioral responses.
— Perform gene expression studies to characterize mechanisms of action.
— Provide toxicity response data in developing zebrafish at a whole transcriptome level.
We spend time getting to know your particular goals and specific testing needs. We advise you on whether our service is a good fit for your project.
Our team of experts go to work designing your custom project and will provide the design within 2 weeks. Our deep knowledge and experience means that we can tackle difficult genetic design problems successfully so that you can get the data you need quickly.
For new clients and new models, we run a custom pilot project. Pilot projects are quick and limited in scope, so that you can be confident that our models and assays will yield useful pre-clinical data.
After approval of a successful pilot project, we undertake the complete project. We provide you with:
• Direct contact with your project manager
• Bi-weekly updates on project status
• A final report including comprehensive data analysis and key take aways
Take advantage of our Zebrafish Knock-In service today and unlock new possibilities for your research! By leveraging the power of CRISPR technology in zebrafish, we offer a comprehensive and efficient approach to introduce precise genetic modifications into your experimental models.
Embrace the possibilities of drug discovery and development. Get in touch with us today and tap into our comprehensive services to revolutionize the landscape of healthcare.
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An expert in CRISPR genome editing, InVivo Biosystems creates custom genome-edited C. elegans and zebrafish models to enable aging, developmental, and disease studies. Our unique in vivo platforms and technologies bridge the gap between cells and mice.
