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Comprehensive Zebrafish CRO Services

Preclinical Drug Discovery & Development With Zebrafish

Zebrafish as a research model has become increasingly popular in recent years due to their small size, transparency during development, and ability to reproduce quickly. While rodent or mice models have been used extensively for research purposes, zebrafish data can complement data from rodent or mice models by providing unique insights into early development, allowing for high-throughput screening, enabling genetic manipulation, facilitating comparative biology, and enabling behavioral studies.

 

By using both models, researchers can take advantage of the strengths of each model for a wide range of biomedical research applications. These complementary data sets can help researchers gain a more comprehensive understanding of biological processes and disease mechanisms, ultimately leading to the development of more effective treatments and therapies for human diseases.

Expert in zebrafish research | InVivo Biosystems

Our Zebrafish CRO Services

Therapeutic Testing | InVivo Biosystems

Therapeutic Testing

Determine if a lead compound ameliorates disease phenotypes or affect important metabolites.

Genetic Target Validation | InVivo Biosystems

Genetic Target Validation

Determine if genes are involved in disease processes and make good targets for further discovery.

Library Screening | InVivo Biosystems

Library Screening

Medium throughput screen of compound libraries and biologics (up to BSL2).

ADME-Tox | InVivo Biosystems

ADME-Tox

Tissue preparation for ADME analysis. General and tissue specific toxicity

Our Core Capabilities

As an expert in zebrafish research, our core competencies reside in the following areas:

  • CRISPR genome editing
  • Deep Phenotyping
  • Omics-based analysis

Our phenotype-based drug discovery process uses zebrafish to bridge the gap between cell culture and murine models. Using zebrafish as an in vivo model, drug discovery can be target independent and results based from the very start.  This approach focuses on ameliorating phenotypes and allows efficacious drugs to be identified more quickly and readily, costing less time and money. And, because of advances in genetics allowing us to reliably express human proteins the zebrafish model is more translational than ever before. Our services are suitable for projects such as:

  • Finding alternative therapeutic indications (aka indication expansion) for your compounds
  • Discovering new therapeutic applications for an existing drug target
  • Discovering novel therapeutics to assist in the treatment of a disease
  • Vetting drug targets identified in GWAS studies

Our Solutions

As a world leader in zebrafish research and services, we can provide end-to-end service including design and line generation, phenotypic data collection and interpretation using the zebrafish model. We provide the following services:

Therapeutics Testing & Evaluation

– Generation of disease models (either chemically or genetically) in order to determine if your lead compound(s) ameliorate disease phenotypes or affect important metabolites.
Disease Model Creation | InVivo Biosystems

Disease Model Creation

— Genetically modified —

— Knockout

— Knock-in

— Point mutation

— Humanized

— Chemical models —

Therapeutic Testing | InVivo Biosystems

Therapeutic Testing

— Small Molecule

— ASO

— mRNA

— Biologics

— Library Screening

Disease Relevant Endpoints Analysis

Disease Relevant
Endpoints Analysis

— Phenotypic

— Molecular

— Protein Analysis

— Imaging

— Gene Expression

— Bioavailability

Genetic Target Validation & Discovery

– Crispant screening: We use CRISPR to generate transient KOs (termed F0 crispants) for a gene of interest. This can be used to rapidly determine whether genes are involved in disease processes and make good targets for further discovery.

Disease Model Creation | InVivo Biosystems

Crispants/ Disrupt Genes

—- Create knockouts in the first generation

—- Disrupt 10-100’s genes

Therapeutic Testing | InVivo Biosystems

Screen Disease
Relevant Endpoints

— Phenotypic

— Molecular

— Protein Analysis

— Imaging

— Gene Expression

Disease Relevant Endpoints Analysis

Identify & Confirm Hits

— Discover which genes are disease relevant

Library & Candidate Screening

– Screen compound libraries and biologics (up to BSL2) for effects on a variety of readouts, including gene expression, fluorescence, and morphometrics.

Disease Model Creation | InVivo Biosystems

Disease Model Creation

— Genetically modified —

— Knockout

— Knock-in

— Point mutation

— Humanized

— Chemical models —

Therapeutic Testing | InVivo Biosystems

Library Screening

— Targeted

— Repurposed drug library

Disease Relevant Endpoints Analysis

Disease Relevant
Endpoints Analysis

— Phenotypic

— Molecular

— Protein Analysis

— Imaging

— Gene Expression

— Bioavailability

Acute Toxicity Testing

– Our Acute Embryo Toxicity testing meets OECD 236 guidelines. We also offer targeted hepatotoxicity, neurotoxicity, and cardiotoxicity analysis.

— Test a range of concentrations, we define concentrations such as the LD50, NOEL, and MNLD.ing.

— Assess Morphological endpoints and Behavioral responses.

— Perform gene expression studies to characterize mechanisms of action.

— Provide toxicity response data in developing zebrafish at a whole transcriptome level.

Other Toxicity Testing

Neurotoxicity Testing

— Hepatotoxicity Testing

— Cardiotoxicity Testing

Our Process

InVivo Biosystems Process #1

Introductory Call

We spend time getting to know your particular goals and specific testing needs. We advise you on whether our service is a good fit for your project.

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InVivo Biosystems Process #2
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Design & Planning

Our team of experts go to work designing your custom project and will provide the design within 2 weeks. Our deep knowledge and experience means that we can tackle difficult genetic design problems successfully so that you can get the data you need quickly.

InVivo Biosystems Process #3

Pilot Project

For new clients and new models, we run a custom pilot project. Pilot projects are quick and limited in scope, so that you can be confident that our models and assays will yield useful pre-clinical data.

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InVivo Biosystems Process #4

Comprehensive Project

After approval of a successful pilot project, we undertake the complete project. We provide you with:
• Direct contact with your project manager
• Bi-weekly updates on project status
• A final report including comprehensive data analysis and key take aways

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Take advantage of our Zebrafish Knock-In service today and unlock new possibilities for your research! By leveraging the power of CRISPR technology in zebrafish, we offer a comprehensive and efficient approach to introduce precise genetic modifications into your experimental models.

Embrace the possibilities of drug discovery and development. Get in touch with us today and tap into our comprehensive services to revolutionize the landscape of healthcare.

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