In Vivo Models
View from the Bench
Why is temperature important for C. elegans?
In this article we discuss the effect temperature has on our favorite worms, and how we can exploit these effects when designing studies.
Trisha Brock
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In this article we discuss the effect temperature has on our favorite worms, and how we can exploit these effects when designing studies.
The development of CRISPR-Cas9 editing has allowed researchers to quickly create precise gene knockouts (KO), but while CRISPR-Cas9 KOs have been highly publicized, are the different methods understood? In this article we will discuss the two available methods for creating knockout models using the CRISPR-Cas9 technology: their advantages and limitations, and how they are being used in research.
Until the 1800s life expectancy across Europe averaged between 30-40 years, now the average life expectancy in the USA is almost 78 years, and our
Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.
We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants were selected from the ClinVar database, literature, the Gnomad database, clinical researchers, and the STXBP1 foundation.
You may or may not know that at InVivo Biosystems, we conduct a lot of grant funded research as well as offering custom models and
Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in zebrafish so we want to share with you what we believe to be the 11 steps necessary to have a successful knock-in project.
A client wanted a knockout (KO) of an embryonic lethal gene. We could not make this line using our standard methods. Instead, we inserted two
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An expert in CRISPR genome editing, InVivo Biosystems creates custom genome-edited C. elegans and zebrafish models to enable aging, developmental, and disease studies. Our unique in vivo platforms and technologies bridge the gap between cells and mice.