Until the 1800s life expectancy across Europe averaged between 30-40 years, now the average life expectancy in the USA is almost 78 years, and our
Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.
We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants were selected from the ClinVar database, literature, the Gnomad database, clinical researchers, and the STXBP1 foundation.
You may or may not know that at InVivo Biosystems, we conduct a lot of grant funded research as well as offering custom models and
Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in zebrafish so we want to share with you what we believe to be the 11 steps necessary to have a successful knock-in project.
A client wanted a knockout (KO) of an embryonic lethal gene. We could not make this line using our standard methods. Instead, we inserted two