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Zebrafish Husbandry 101: Embryo Injections

How do we inject zebrafish embryos? This quick video gives a great summary for how we perform zebrafish embryo injections and some tips and tricks so you can ace your next injections too.

Interested in learning more? You can view the rest of our Zebrafish Husbandry 101 series here, and learn more about our zebrafish services here.

Transcript

After we collect the embryos, we often inject them. Here we are loading up an agar plate that has been molded to form channels that the embryos can rest in. The channels have a vertical side and a side that’s at a 45 degree angle. When we place the embryos in the channel, we can inject towards the vertical side. Here you can see a single celled embryo in our small needle poking through the center and depositing the injection material.

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InVivo Biosystems

InVivo Biosystems provides essential services to help pharmaceutical, nutraceutical, biotechnology companies and academic research institutions around the globe accelerate their research and drug development efforts. An expert in CRISPR genome editing, InVivo Biosystems creates custom genome edited C. elegans and zebrafish models to enable aging and other disease studies. In addition, InVivo Biosystems provides in-vivo analytical services to produce data and insights for companies that need to make go/no-go decisions quickly in early-stage development of new compounds.

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