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Article Review: The Caenorhabditis elegans Transgenic Toolbox

C. elegans are becoming known as an ideal model for transgenics research. Despite being microscopic in size, due to the similarities with humans, the small worm can be used in the lab to research the ways in which transgenics can prevent and cure genetic disorders. 

Even though C. elegans are popular among transgenics researchers, the many ways in which CRISPR/Cas9 and transposon-mediated transgenesis can be used to modify the genome of C. elegans are not as widely understood. 

This article offers an updated, comprehensive listing of the best techniques available for C. elegans transgenics, as well as experimental concerns that should be considered with each method. Each technique provides new amounts of data, precision, and control to the gene-editing process through the insertion of conditional promoters.

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Sophia Bethel

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