In Vivo Models
Miron Mikhailowitsch Gershkovich, Victoria Elisabeth Groß, Anette Kaiser & Simone Prömel. Cell Communication and Signaling. Volume 17, Article number: 123 (2019).
July 22, 2022
Resources
InVivo BioSystems provides a specialized service to biotechnology researchers involved in Caenorhabditis elegans transgenic research. With the use of cutting-edge CRISPR technologies, the team is able to offer accurate and efficient C. elegans custom injection service. CRISPR injection services are tailored to the specific needs and preferences of each client, allowing for customized and precise enhancement of genome editing.
Below are the benefits of InVivo Biosystems’ custom injection service for C. elegans transgenic research using CRISPR technology.
C. elegans
InVivo Biosystems’ types of injection services:
Examples of successful (left) and unsuccessful (right) injections. The video captures COP1403 (unc-18) injections.
What Do You Receive
One set of 10 worms, injected with a
plasmid or DNA mix prepared and provided
by you
Professionally prepared injection mix containing all of the required molecular components to achieve your desired custom genetic edit.
Expertise Needed
(Pre-Service)
Ability to generate DNA mix and/or plasmid constructs for injection.
None
Expertise Needed
(Post-Service)
Ability to screen and select final desired strain(s).
- Technical skill for proper injection;
- Access to microinjection rig;
- Ability to screen and select final desired strain(s).
Your Molecular Design Time
High
Low
Your Injection Time
Low
High
Inject Express *
Starts at $400/set
1 week**
Custom Injection Mix***
Starts at $1,050
2 weeks and up
You provide the gene editing idea, we design the reagents, complete the injections and provide animals who have passed our first round of screening.
Need an alternative C. elegans strain injected? We can do it! We need an additional week to grow a population suitable for injection. A Strain Preparation fee will be applied once per strain.
Note: The option to inject into N2, unc-119 (ed3) null, unc-18 null, or various MosSCI strains (ttTi5605, cxTi10882, ttTi14024) is included at no additional charge.
Due to temperature requirements, we recommend that we keep your miniMOS projects at our facility post injection (as opposed to shipping the following day) to ensure a higher chance of success. Heat shock screening and shipping of surviving lines can be completed for an additional fee.
Note: In the event of low transgenic yield, we are happy to provide troubleshooting for your injection mix.
Miron Mikhailowitsch Gershkovich, Victoria Elisabeth Groß, Anette Kaiser & Simone Prömel. Cell Communication and Signaling. Volume 17, Article number: 123 (2019).
Structural Principles in Robo Activation and Auto-inhibition.
Barak R, Yom-Tov G, Guez-Haddad J, Gasri-Plotnitsky L, Maimon R, Cohen-Berkman M, McCarthy AA, Perlson E, Henis-Korenblit S, Isupov MN, Opatowsky Y. Cell. 2019 Apr 4;177(2):272-285.e16.
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An expert in CRISPR genome editing, InVivo Biosystems creates custom genome-edited C. elegans and zebrafish models to enable aging, developmental, and disease studies. Our unique in vivo platforms and technologies bridge the gap between cells and mice.