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Express and Custom C. elegans CRISPR Injection Services

Efficient CRISPR Injection Service for C. elegans Transgenic Research

InVivo BioSystems provides a specialized service to biotechnology researchers involved in Caenorhabditis elegans transgenic research. With the use of cutting-edge CRISPR technologies, the team is able to offer accurate and efficient C. elegans custom injection service. Each CRISPR injection services are tailored to the specific needs and preferences of each client, allowing for customized and precise enhancement of genome editing.

Efficient CRISPR Injection Service For C. Elegans Transgenic Research | InVivo Biosystems

Why Choose Our C. elegans Inject Express Service?

Below are the benefits of InVivo Biosystems’ custom injection service for C. elegans transgenic research using CRISPR technology.

  • Customized injection protocols tailored to your specific research needs
  • Use of the latest CRISPR technology for precise genome editing
  • Experienced technicians and scientists with a proven track record of success in CRISPR injection and C. elegans transgenic research
  • Competitive pricing with no hidden fees
  • Efficient and timely delivery of results
  • Committed to providing our clients with the best possible C. elegans custom injection service to help them achieve their research goals with precision and efficiency.

CRISPR Injection Services

InVivo Biosystems’ types of injection services:

Inject Express Service Details

Inject Express is a fast and affordable way to turn your prepared plasmids into C. elegans strains. You provide the injection mix and we complete the injections and return the worms to you for screening in less than a week.

  • Send us your plasmid and we will prepare it using our proprietary clean-up method proven to increase transgenic yield.
  • We will then complete your injection mix with your choice of co-markers.
  • Injections are completed in sets of 10 worms.
  • Injected worms are checked at 24 hours post injection for survival, and all surviving animals are sent to the client.
  • We recommend 1 set of worms injected for extrachromosomal array projects and 3 sets of worms injected for MosSCI and CRISPR projects.
  • The Injection Mix Preparation fee applies to each injection mix.

InVivo Biosystems’ types of injection services:

Custom Injection Mix Service Details

  • Send us your gene editing idea.
  • Our expert staff will work collaboratively with you to design an injection mix that meets your needs.
  • We prepare reagents containing all of the required molecular components to achieve your desired custom genetic edit

Successful vs. Unsuccessful Injections

Examples of successful (left) and unsuccessful (right) injections. The video captures COP1403 (unc-18) injections.

C. Elegans Custom Injection Service Process

  1. Strain Growth & Materials Receiving:

    Your injection materials are cataloged upon receiving in our lab, and your strain is maintained under proper conditions by our expert staff in preparation for injection.

  2. Microinjection Of Adult Gonads:

    Our experienced injectionists perform microinjections to your specifications.

  3. Survival Verification:

    We confirm the survival of the injected animals 24 hours after injection and then prepare for shipping.

Inject Expess Service Process

OFFERING
INJECT EXPRESS
CUSTOM INJECTION MIX

What Do You Receive

One set of 10 worms, injected with a
plasmid or DNA mix prepared and provided
by you

Professionally prepared injection mix containing all of the required molecular components to achieve your desired custom genetic edit.

Expertise Needed

(Pre-Service)

Ability to generate DNA mix and/or plasmid constructs for injection.

None

Expertise Needed

(Post-Service)

Ability to screen and select final desired strain(s).

- Technical skill for proper injection;
- Access to microinjection rig;
- Ability to screen and select final desired strain(s).

Your Molecular Design Time

High

Low

Your Injection Time

Low

High

CRISPR Injection Service Pricing

OFFERING
PRICING
DELIVERY TIME

Inject Express *

Starts at $400/set

1 week**

Custom Injection Mix***

Starts at $1,050

2 weeks and up

NOTE: Additional charge applies for large constructs (determined by project type). Prices above do not include shipping charges; you may opt to use your own shipping account and/or provide a prepaid shipping label.
*Inject express denotes one set of 10 injected worms.
**Inject express using a client-provided strain will add 1 week to the total injection project timeline, for a final timeline of approximately 2 weeks.
***Custom injection mix is available for any of our standard service offerings and edit types, including CRISPR-based edits, MosSCI edits, Extrachromosomal Arrays and tagging.
Overnight Shipping:
Preferred: you provide your own FedEx, UPS, or DHL account or a pre-paid label.
Ship via FedEx by Invivo Biosystems: $100 domestic; $200 international. Due to shipping time requirements, USPS cannot be used to ship Inject Express projects.

Other Gene Editing Service We Provide

Other Transgenic Injection Services

  • Candidate lines service

You provide the gene editing idea, we design the reagents, complete the injections and provide animals who have passed our first round of screening.

Need an alternative C. elegans strain injected? We can do it! We need an additional week to grow a population suitable for injection. A Strain Preparation fee will be applied once per strain.

Note: The option to inject into N2, unc-119 (ed3) null, unc-18 null, or various MosSCI strains (ttTi5605, cxTi10882, ttTi14024) is included at no additional charge.

Candidate lines service | InVivo Biosystems
  • MiniMOS

Due to temperature requirements, we recommend that we keep your miniMOS projects at our facility post injection (as opposed to shipping the following day) to ensure a higher chance of success. Heat shock screening and shipping of surviving lines can be completed for an additional fee.

Note: In the event of low transgenic yield, we are happy to provide troubleshooting for your injection mix.

MiniMos | InVivo Biosystems

Inject Express Publications

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