RapidGen^TM Model

Your Fast Lane to Genetic Insights

Transforming Genetic Modeling With Speed and Precision

Make critical decisions about your genetic or disease modeling strategy in a fraction of the time it takes to create an animal model.

The RapidGen^TM Fish Model uses a novel crispant approach to rapidly screen for functional genetic models before committing to building an animal strain. A stable zebrafish genetic model typically takes 9-12 months to construct using standard CRISPR approaches, not including the time it takes to test and validate the new model.

This investment of time and resources is incompatible with the fast pace of new genetic association discovery. The RapidGen^TM Fish model provides early proof-of-concept or viability testing of your model in as little as one month.

Figure 1. RapidGen^TM Model of Macrophage Knockout in Zebrafish. Knockout of the irf-8 gene that encodes Interferon Regulating Factor 8 which promotes macrophage development. (A) Uninjected sibling control Zebrafish embryos at 48 hours post injection with macrophages illuminated by expression of mfap4:GFP. (B) RapidGen^TM Model fish injected with irf-8 Cas9 and multiple sgRNAs showing visible reduction of GFP+ macrophage cells. Co-injected with Rhodamine dye to indicate successful injection.

Applications of RapidGen^TM Fish Model

Decide Early whether to invest time and resources into a genetic model.
Rapid Target Validation. Determine whether your model has a disease specific phenotype.
Quickly Screen Multiple Targets for biological function.
Easily Probe Functional Domains of your gene of interest.

Advantages of RapidGen^TM Fish Model

Save Time and Resources by ensuring your model has a disease specific phenotype before committing to full strain construction.
Scalable for high throughput screening and validation of candidate target genes.
Therapeutic Testing Compatible.
Edits are Permanent, unlike morpholinos, so animals can be assayed at all growth stages.
Can be Propagated to create a stable line.

How does RapdiGen^TM work?

InVivo Biosystems optimized the crispant approach to efficiently “break” a gene at multiple sites using multiple sgRNAs, particularly in the context of zebrafish knockout studies.. The result is a highly penetrant gene knockout that can be assayed by phenotype immediately in the F0 animals (Figure 1). A traditional CRISPR-based edit modifies a single site in a gene and requires months of propagation and screening to stabilize and confirm the edit before assaying. Unlike transient knockdowns, such as morpholinos, the crispant modifications are permanent and can be propagated to establish a stable line.

Deliverables

Because the RapidGen^TM approach creates a transient model, phenotypic analysis is performed by InVivo Biosystems and animals are not delivered to the customer. Based on the phenotypic screen chosen, deliverables might include:

Raw phenotypic data, including images.
Expert data analysis and interpretation.
Successful crispants can be recovered and converted to a strain build project.
Successful genetic modeling can be prepared for translation into a mouse model line.

Other Offerings

Hepatotoxicity Testing

Determine whether a compound or formulation induces liver damage. Measurements using early life-stage zebrafish include gross tissue health, necrosis, liver function and cell death.

Neurotoxicity Testing

Assess whether a compound or formulation induces nervous system damage. Early life-stage zebrafish will be used to measure neural gene expression via qPCR.

Cardiotoxicity Testing

Assess whether a compound or formulation induces heart damage in zebrafish. Early life-stage zebrafish will be used to assess cardiac function.

Our Process

Introduction

Understand Your Goal

We will set up a 30-minute call with you to understand your needs. During this call, we will define the scope of your project, answer your practical questions and help you assess whether our service is a good fit for you.

Design

Create A Custom Catalog

After we decide on the scope of the project, we will create a proposal that includes cost and time estimate for each experiment proposed. You will be able to personalize your project plan.

Logistics

Finalize Project Design

We will send you a final statement of work and payment schedule. Once we receive your first PO, we will start the experiments and give you a defined timeline for your project.

Report Out

Maintain Clarity And Transparency

We will keep you updated every two weeks on the status of your project. At the end of your project, we provide you with a report that includes comprehensive findings and key takeaways.

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RapidGenTM Model