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Looking at Gene Paralogs across both zebrafish and C. elegans Model Organisms [Customer Story]

Dr David Pruyne and Dr Jeff Almack are Associate Professors of Cell and Developmental Biology at Upstate Medical University. Dr Prunye’s lab primarily utilizes a C. elegans model system to study muscle cell growth, but recently teamed up with Dr Almack to investigate whether vertebrates show similar effects. 

Pruyne and Almack wanted to disrupt the genes fhod3a and fhod3b in zebrafish, as they are associated with familial hypertrophic cardiomyopathy – a condition where heart muscle grows abnormally thick and makes it hard for the heart to pump. 

But, when working with these genes it is necessary to target very specific point mutations within the exons because these genes are subject to alternative splicing which, in turn, can obscure the isoform specific effect. 

Pruyne and Almack leveraged InVivo Biosystems’ Custom Validated Injection Mix offering to design and create a validated injection mix for the generation of a novel zebrafish line carrying a targeted in frame deletion to disrupt fhod3a and fhod3b. 

Almack & Pruyne Customer Story

Download Almack & Pruyne Customer Story to learn more about Dr. Pruyne and Dr Almack’s research, why they chose to leverage the InVivo Biosystems’ Custom Validated Injection Mix offering, and the benefits of using our platform.

About The Author

Alexandra Narin

Alexandra is a Content Marketing Specialist and Grant Writer for InVivo Biosystems. She graduated from the University of St Andrews in 2020 where she earned a Joint MA Honours Degree in English & Psychology/Neuroscience with BPS [British Psychology Society] Accreditation. She has worked as a research assistant, examining the LEC's (lateral entorhinal cortex) involvement in spatial memory and integrating long term multimodal item-context associations, and completed her dissertation on how the number and kinds of sensory cues affect memory persistence across timescales. Her hobbies include running, boxing, and reading.

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