Using Zebrafish Custom Injection Mixes to Accelerate the Grant Application Process

CRISPR, Customer Story, Drug Discovery
By: Alexandra Narin ,

Our custom injection mix services utilize our patented CRISPR technology to enable specific protein point mutations. This customer story shows how our validated injection mixes served as proof-of-concept during the grant application process.

Dr Anita Quintana is an Associate Professor in Biological Sciences at the University of Texas at El Paso. Her lab is focused on understanding the mechanisms underlying multiple congenital anomaly syndrome. They utilize a zebrafish animal model to study gene mutations which they’ve identified in patients in the clinic.

Dr Quintana approached InVivo Biosystems, an industry-leading Zebrafish CRO, wanting to collaborate on three independent projects that each targeted a different region or domain of the protein HFC1. HFC1 is of particular interest to Quintana because it is a gene that is associated with intellectual disability and manifests during the developmental period. The end goal of the three projects was to compare how different gene mutations would differ in their disruption of neural precursors.

Dr. Quintana chose to leverage InVivo Biosystems’ fully validated custom injection mixes to create point mutations that target the protein HFC1 in zebrafish. InVivo Biosystems worked with Dr. Quintana’s lab to design the mixes, and provided her lab with the validation protocol. Consequently, Quintana’s lab was able to generate F1 heterozygous carriers from the fully validated mixes, and then, used InVivo’s screening reagents to identify carriers with the precise germline edit within a matter of weeks.

Read More: Quintana Customer Story

Download Dr Quintana’s Customer Story to learn more about her research, why she chose to leverage InVivo Biosystems’ fully validated custom injection mixes, and the benefits of using our platform.Download Dr Quintana’s Customer Story to learn more about her research, why she chose to leverage InVivo Biosystems’ fully validated custom injection mixes, and the benefits of using our platform.

About The Author

Alexandra Narin

Alexandra is the Marketing Content Manager and Grant Writer for InVivo Biosystems. She graduated from the University of St Andrews in 2020 where she earned a Joint MA Honours Degree in English & Psychology/Neuroscience with BPS [British Psychology Society] Accreditation. She has worked as a research assistant, examining the LEC's (lateral entorhinal cortex) involvement in spatial memory and integrating long term multimodal item-context associations, and completed her dissertation on how the number and kinds of sensory cues affect memory persistence across timescales. Her hobbies include running, boxing, and reading.

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About the Author:

Alexandra Narin