Basics Of Zebrafish Husbandry

Zebrafish Husbandry: Essential Guide To Optimize Breeding And Housing Conditions

Have you ever wanted to learn more about how we take care of our zebrafish, starting your own rack, what to feed your zebrafish, or anything zebrafish husbandry related? You have come to the right place. We have put together a series of educational videos to help answer your zebrafish husbandry questions and give you an inside look into our work here at InVivo Biosystems.

Zebrafish Facilities Tour

Want to learn more about our own zebrafish facilities? We put together a lab tour (~19 minutes in length) to provide you with an overview of our facilities in addition to sharing more about how we care and maintain our fish. Some of the sections in this video can also be found below in condensed versions.

Maintaining water quality in your tanks

Maintaining water quality is crucial for successful zebrafish maintenance and husbandry, especially during the startup of a fish rack. This video discusses how to keep track of the water quality parameters (ammonia, nitrites, nitrates, and ph), gives an example of how we started our fish rack, and the process of the nitrogen cycle for initial rack set-up.

Zebrafish Screening Using Fluorescence

How can you better study your gene of interest? Fluorescent tagging! This video showcases InVivo Biosystems’ fluorescent screening process using zebrafish. Watch the video for example photos of embryos that have fluorescent markers.

Zebrafish feeding: Live vs. dry food

What type of food should you feed your zebrafish? Optimally, zebrafish will be fed live food and dry food. This video provides a look at food portioning and feeding schedule, and how we grow and maintain our live food (rotifers).

Tracking Zebrafish Health

This video discusses the daily maintenance and upkeep of zebrafish, including keeping track of population health, our daily zebrafish rack checklist (water flow, buffer fills, etc.), and the fishes’ feeding schedule.

Cleaning & Disinfecting The Tanks

An important (but time-consuming) aspect of zebrafish maintenance and husbandry is the proper cleaning and disinfecting of lab equipment. Tanks should be cleaned every 5-6 weeks — this video walks you through the handling of zebrafish during tank to tank transfers, and the cleaning of the old tank.

Zebrafish Crossing and Breeding Strategies

Are you wanting to learn more about zebrafish breeding? Have you ever wondered how to tell the males from the females when they’re all swimming around? Our zebrafish team put together this insightful video to show the process of breeding zebrafish and sexing.

Zebrafish Crossing Basics

This video provides a brief overview of the zebrafish breeding process: first, the fish should be sexed then placed in a tilted breeding container to simulate their natural environment and allow for the easy collection of embryos.

Zebrafish Crossing In Depth

This video is a step-by-step guide to breeding zebrafish, also called zebrafish crossing. It is an in-depth look into zebrafish husbandry, covering the process of breeding from selecting mates, to collecting embryos, the injection of transgenic materials and dye, cleaning the plates, screening, and finally, prepping for PCR (polymerase chain reaction)

Zebrafish Embryo Injections

How do we inject zebrafish embryos? This quick video gives a great summary for how we perform zebrafish embryo injections and some tips and tricks so you can ace your next injections too.

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