The nematode Caenorhabditis elegans has provided remarkable insights into the biology of aging. The precisely measurable lifespan of worms has proven to be an efficient tool to assess the impact of various genetic, physiological and environmental factors on organismal aging. Lifespan assays can be labor intensive and time consuming. In this article, we will highlight the critical considerations that could make or break your lifespan assay. Our aim is to help you interpret a variety of different procedures by emphasizing the crucial considerations to ensure the success of running a lifespan assay.
In this whitepaper, we will highlight the critical considerations that could make or break your lifespan assay, including:
- Automated vs. manual lifespan assays
- Food, nutrition, and contamination
- Temperature control
- Progeny suppression
- Scoring and operators
- experimental design and analysis