A Shortcut to Understanding Longevity
In Vivo Lifespan & Healthspan Analysis
The InVivo Biosystems Longevity Platform enables us to determine your compound’s ability to extend lifespan and healthspan in only 3 months.
Many of our clients want to test new compounds and formulations to understand whether and how they modulate behavioral and molecular changes.
We work with our clients to define and choose the most appropriate tests to assess the effects of their compounds. Our goal is to provide fundamental insights into the effects and mechanisms of action of their compounds in a way that can be relevant to humans.
Gain Insights into the Mechanism of Action within 3 months
Traditional longevity experiments using mice can take upwards of 2-3 years to complete and cost hundreds of thousands of dollars. The InVivo Biosystems Longevity Platform is designed to: 1) quickly and economically test a compound’s ability to extend lifespan and healthspan in a whole animal model; 2) characterize the effect of the compound on known aging pathways including mitochondrial, oxidative stress, and DNA damage.
Aging pathway analysis. Global gene expression analysis indicates two candidate pathways likely to mediate the effect of compound on lifespan: oxidative phosphorylation and oxidative stress.
Comprehensive analysis from data collected over the entire lifespan of the animal
The InVivo Biosystems Longevity Platform produces a comprehensive analysis using the data collected over the entire lifespan of the adult animal. Morphology and movement information are used to extract 7 measurements of vitality.
InVivo Biosystems Multi-dimensional Functional Analysis: data modeling for an analysis to measure the 2 most impactful measurements: mobility and morphology.
The in-vivo longevity platform is an effective approach to test the effects of a compound on lifespan, healthspan, and transcriptional changes related to aging. Our 3-step approach provides insights into the mechanism of action for lifespan and healthspan.
- Step I: Conduct a dose and toxicity assessment of the compound to determine whether a full longevity experiment is feasible and at what dosage. (Fig. 1)
Dose-response curve to determine the stress level introduced by the compound. Day 1 adult worms were treated with Lu0128 at 1µM, 10µM, 100µM, 1mM, and 10mM for 12 hours. A) Xenobiotic stress biosensor response assay. B) Oxidative stress response assay. Calculated working EC50 = 21µM.
- Step II: Measure the lifespan and vitality extending effects of the compound by monitoring the adult animal’s survival, morphology and movement. The healthspan metrics are extracted from the same dataset as the lifespan readings.
Assessing the eligibility of 2 promising compounds. (A) Compound A increases lifespan (orange) compared to untreated control. (B) In addition, exposure to compound A triggers a considerable improvement of healthspan, with 20-day old worms exposed to compound A exhibiting an overall activity level similar to untreated worms (grey) that are only 12-day old. Unlike control populations, worms exposed to compound A do not exhibits complete loss of activity prior to death. (C) Compound B also shows a strong longevity effect (purple), but (D) triggers a sharp, early decline in healthspan leading to a complete loss of locomotion by Day 23.
- Step III: Identify the aging pathways that contribute to the observed lifespan extension from Step II.
Measure changes in gene expression level. The gene asp-12 is downregulated 117-fold (p=1.3e-37) in the day 10 Lu0128 treated samples compared with the day 10 untreated. The gene lea-1 is upregulated 6.5-fold (p=2.4e-16) in the day 10 Lu0128 treated samples compared with the day 10 untreated.
Download our sample data report to see an example dataset you would receive if you partnered with us.
Frequently Asked Questions
The dose finding test uses proprietary and patented C. elegans biosensor strains to monitor the physiological effect of a compound on the animal.
We monitor and measure the growth rate of animals from hatching to the first day of adulthood (total of 4 days).
WTS can be more cost-effective than qPCR to identify which genomic loci are expressed in a population at a given time over the entire expression range, hypothesis-free (without the need to pre-define the genes of interest). In addition, WTS yields high dimensional data for a large number of genomic loci and detects gene expression changes (i.e., mRNA levels) between different experimental conditions.
We accept crystals, emulsions, oils, nanoparticles or nanobeads up to 15 µm diameter.
We do not accept compounds that are hazardous and/or BSL 3 and above or any hazardous volatile compounds that require handling in a chemical hood.
Yes, however we recommend that a client provide either the compound itself or the specific catalog number(s) of the compound to be tested.
A positive and a negative control are included for each compound. The appropriate controls will be defined based on the nature of the compounds to be tested.
Understand Your Goal
We will set up a 30-minute call with you to understand your needs. During this call, we will define the scope of your project, answer your practical questions and help you assess whether our service is a good fit for you.
Create A Personalized Catalog
After we decide on the scope of the project, we will create a proposal that includes cost and time estimate for each experiment proposed. You will be able to personalize your project plan.
Finalize Your Project Design
We will send you a final statement of work and payment schedule. Once we receive your first PO, we will start the experiments and give you a defined timeline for your project.
Maintain Clarity And Transparency
We will keep you updated every two weeks on the status of your project. At the end of your project, we provide you with a report that includes comprehensive findings and key takeaways.