Lifespan and Health Analysis

In Vivo Longevity Platform

We offer a streamlined Longevity Platform to determine whether a compound can
extend lifespan, promote a longer healthspan, and test the outcome on signaling
pathways and cellular mechanisms promoting longer life.

We specialize in giving our clients a full picture of how each compound impacts
several key pieces of the aging puzzle: the overall lifespan, the activity level and
muscle use throughout senescence, and the underlying transcriptional markers of
health. Our detailed data reports sets us apart. See an example report now.

Our data helps companies prioritize their development pipelines by giving them
quantitative information about how candidate formulations combat aging and age-
related conditions such as sarcopenia.

Kaplan-Meier curve showing the effects of two compounds on the lifespan of a population of animals. The curve depicts the decline in population as time progresses. The population is assessed 3 times each hour for 40 days. Animals treated with Compound A have a significantly longer lifespan than the animals treated with vehicle control. Animals treated with Compound B have a shortened lifespan.

Webinar:
A Shortcut To Understanding Longevity: A Rapid In Vivo Analysis Of A Compound’s Effect On Health And Aging

Advantages

Traditional longevity experiments using mice can take upwards of 2-3 years to complete and cost hundreds of thousands of dollars. Using our platform, you can quickly and economically test a compound’s ability to extend lifespan and healthspan with large sample sizes of live animals in less than 5 months and get answers to the following questions:

  • Does my compound have positive or negative effect on lifespan?
  • How does my compound affect lifespan AND healthspan?
  • Which are the associated signaling pathways that attributed to the life extension?

Get Kaplan-Meier survival curves from live animals within 5 months.

Using our platform, you can quickly and economically test a compound’s ability to extend lifespan and healthspan with large sample sizes of live animals. We use automated scanning to ensure smooth survival curves and robust conclusions.

Leverage Molecular Biology to Understand Mechanism of Action.

We use RNAseq to help our clients get the “big picture” effect of their compound on known aging pathways including mitochondrial, oxidative stress, and DNA damage. By comparing the complete gene expression profile of animals as they age, we can uncover how compounds exert their lifespan-extending effects.

Aging pathway analysis. Global gene expression analysis indicates two candidate pathways likely to mediate the effect of compound on lifespan: oxidative phosphorylation and oxidative stress.

Mapping of gene expression data to longevity pathways in the KEGG database. Pathway shown represents changes of expression that might be observed under conditions of dietary restriction. Pathway components are color coded based on the level of up or down regulation of treated samples versus control. 

Our Approach

Our In vivo Longevity Platform is an effective approach to test the effects of a compound on lifespan, healthspan, and transcriptional changes related to aging. Our 3-step approach provides insights into the mechanism of action for lifespan and healthspan.

Step I: Toxicity Assessment

Viability, Growth, and Development assays are used to determine toxicity levels in C. elegans.

Viability, Growth and Development Assay to determine toxicity of Lu0128 in C. elegans. Left: Violin plot showing distribution of worm lengths during development from larvae to adult when treated with varying concentrations of Lu0128. Right: Day 3 snapshot of worm development including embryonic lethality (black).

Step II: Measuring lifespan

Measure the lifespan and vitality extending effects of the compound by monitoring the adult animal’s survival, morphology and motility. The healthspan metrics are extracted from the same dataset as the lifespan readings.

Aggregate motility and morphology analysis over duration of lifespan. Representative data from worms treated with either vehicle (Control) or a compound that improves healthspan (Drug).  All measures are obtained by averaging data for all worms detected on a plate, then averaging across different replicate plates of the same condition. All measurements are based on worms that are still alive and moving at the time of quantification. Motility measures are normalized to fall between 0 and 100.

Step III: Identifying Aging Pathways

Identify the aging pathways that contribute to the observed lifespan extension from Step II. Potentially identified pathways and mechanisms are identified, including genes related to mTOR, sirtuin, dietary restriction, oxidative stress.

Mapping of gene expression data to longevity pathways in the KEGG database. Pathway shown represents changes of expression that might be observed under conditions of dietary restriction. Pathway components are color coded based on the level of up or down regulation of treated samples versus control. 

This method holistically maps gene expression data to both established longevity pathways and peripheral pathways that support longevity. Colored score increments indicate level of up-(red) or down-(blue) regulation weighted by the statistical significance. Solid lines indicate direct pathway connections and dashed lines indicate indirect or multi-step connections. Where lists of many differentially-expressed genes have been condensed into a module, the number of genes is indicated.

Measure changes in gene expression level. The gene asp-12 is downregulated 117-fold (p=1.3e-37) in the day 10 Lu0128 treated samples compared with the day 10 untreated. The gene lea-1 is upregulated 6.5-fold (p=2.4e-16) in the day 10 Lu0128 treated samples compared with the day 10 untreated.

Customer Testominal

"I really felt that InVivo Biosystems did pretty much everything that they said they would do. It was done in a manner that gave me confidence in the results. The communication and collaboration were as good as I've ever been a part of. If I wanted to do another study, I'd certainly have no problem coming back."  – Dr. David Vollmer, Chief Scientific Officer,  4Life Research. Read the customer story. >>

Service Packages

Our in-vivo Longevity Platform offers 3 service packages. Choose the package(s) that serves your needs the best.

  • Lifespan and healthspan analysis: produce lifespan and healthspan measurements by automatically monitoring an adult C. elegans’ survival, morphology and movement using the data collected over the entire lifespan of the adult C. elegans. 
  • Pathway analysis: identify the pathways that contribute to the observed lifespan extension using RNAseq or whole transcriptome sequencing (WTS).
  • Compound testing: determine a compound’s ability to extend lifespan and healthspan within 4 months once an experiment starts.

Resources

Sample Report

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Download our sample report to see examples of data we can generate. 

Case Study

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How a company leveraged C. elegans to predict the most valuable compound to develop.

Frequently Asked Questions

The dose-finding test uses proprietary and patented C. elegans biosensor strains to monitor the physiological effect of a compound on the animal.

We monitor and measure the growth rate of animals from hatching to the first day of adulthood (total of 4 days).

WTS can be more cost-effective than qPCR to identify which genomic loci are expressed in a population at a given time over the entire expression range, hypothesis-free (without the need to pre-define the genes of interest). In addition, WTS yields high dimensional data for a large number of genomic loci and detects gene expression changes (i.e., mRNA levels) between different experimental conditions.

We accept crystals, emulsions, oils, nanoparticles or nanobeads up to 15 µm diameter.

We do not accept compounds that are hazardous and/or BSL 3 and above or any hazardous volatile compounds that require handling in a chemical hood.

Yes, however we recommend that a client provide either the compound itself or the specific catalog number(s) of the compound to be tested.

Three.

A positive and a negative control are included for each compound. The appropriate controls will be defined based on the nature of the compounds to be tested.

Our Process

Introduction

Introduction

Understand Your Goal

We will set up a 30-minute call with you to understand your needs. During this call, we will define the scope of your project, answer your practical questions and help you assess whether our service is a good fit for you.

Procedures

Design

Create A Custom Catalog

After we decide on the scope of the project, we will create a proposal that includes cost and time estimate for each experiment proposed. You will be able to personalize your project plan.

Technology

Logistics

Finalize Project Design

We will send you a final statement of work and payment schedule. Once we receive your first PO, we will start the experiments and give you a defined timeline for your project.

Nemametrix-icons-set2-Results

Report Out

Maintain Clarity And Transparency

We will keep you updated every two weeks on the status of your project. At the end of your project, we provide you with a report that includes comprehensive findings and key takeaways.

READY TO GET STARTED?

Ready to connect with us to learn more about working with us on compound testing?

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