C. elegans Point Mutation Service

Our Point Mutation service uses CRISPR/Cas9, which is the best gene editing method for creating small, precise edits to introduce a small number of nucleotide changes at a target site. With this service you can:
- study a disease-causing mutation
- humanize a critical amino acid
- explore the binding site of an enzyme
- introduce phosphomimetics
- mutate isoform start sites or make any specific mutation of interest.
Our CRISPR/Cas9 editing is typically very efficient. Among the F1 candidates with the Co-CRISPR edit, our average percentage of animals with the target edit was 65.7%. Edit percentages as high as 95% were observed.
96 point mutations in 1 gene. All 96 mutations were created in the STXBP1 gene (which is associated with epilepsy in humans) via CRISPR. The worm homolog of STXBP1, unc-18, causes uncoordination and near-complete lack of pharyngeal pumping when knocked out. The functionality is restored by replacing the worm gene with the coding sequence for human STXBP1.
Service Details (price reflects academic pricing)
Service Package | Price | Est. Delivery Time |
---|---|---|
Full Build | $4,075 - $4,360 | 6 - 8 Weeks |
Candidate Lines | $2,554 - $2,839 | 3 - 4 Weeks |
Custom Injection Mix | $995 - $1,295 | 1 - 2 Weeks |
Point Mutation Publications
Repurposing the aldose reductase inhibitor and diabetic neuropathy drug epalrestat for the congenital disorder of glycosylation PMM2-CDG
Sangeetha Iyer, Feba S. Sam, Nina DiPrimio, Graeme Preston, Jan Verheijen, Kausalya Murthy, Zachary Parton, Hillary Tsang, Jessica Lao, Eva Morava2 and Ethan O. Perlstein. Disease Models & Mechanisms (2019) 12.
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