C. elegans Mutation Packages

C. elegans Point Mutation Service

showcase_point-mutation

The Point Mutation service uses CRISPR/Cas9 to introduce a small number of nucleotide changes at a target site. With this service you can:

  • study a disease-causing mutation
  • humanize a critical amino acid
  • explore the binding site of an enzyme
  • introduce phosphomimetics
  • mutate isoform start sites or make any specific mutation of interest.

See how researchers used this service to create four point mutations in the RNase domain of the rege-1 gene, leading to the discovery of the role of rege-1 in regulating fat metabolism (PMID:27746047 ).

Service Details (price reflects academic pricing)

Service PackagePriceEst. Delivery Time
Full Build $4,075 - $4,3606 - 8 Weeks
Candidate Lines $2,554 - $2,8393 - 4 Weeks
Custom Injection Mix $995 - $1,295
1 - 2 Weeks

Point Mutation Publications

  • Repurposing the aldose reductase inhibitor and diabetic neuropathy drug epalrestat for the congenital disorder of glycosylation PMM2-CDG

    Iyer S, Murthy K, Parton Z, Tsang H, Sam FS. bioRxiv. 2019 May 3; 1-29

  • Regulation of the sperm-to-oocyte transition in Caenorhabditis briggsae hermaphrodites by the Cbr-met-2 and Cbr-fem-3 genes

    Berenson AL, Baird SE. Mol Reprod Dev. 2018 Jun;85(6):532-542.

  • NALCN channelopathies: Distinguishing gain-of-function and loss-of-function mutations

    Bend, EG; Si, Y; Stevenson, DA; Bayrak-Toydemir, P; Newcomb, TM; Jorgensen, EM; Swoboda, KJ. Neurology. 2016 Sep 13;87(11):1131-9.

  • An RNAi-based suppressor screen identifies interactors of the Myt1 ortholog of Caenorhabditis elegans

    Allen, AK; Nesmith, JE; Golden, A. G3 (Bethesda). 2014 Oct 8;4(12):2329-43.

  • LEM-3 is a midbody-tethered DNA nuclease that resolves chromatin bridges during cytokinesis

    HONG, Y; Sonneville, R; Wang, B; Scheidt, V; Meier, B; Woglar, A; Demetriou, S; Labib, K; Jantsch, V; Gartner, A. Nat Commun. 2018 Feb 20;9(1):728.

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