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C. elegans MosSCI Service for Precise Transgene Integration

InVivo Biosystems is one of the pioneers in precision genetic manipulation providing unparalleled precision with Mos1-mediated Single Copy Insertion (mosSCI). Our groundbreaking techniques enable the precise insertion of transgenes into specific locations within the Caenorhabditis elegans genome. Harnessing the power of the Mos1 transposase, we induce a targeted double-strand break in genomic DNA, seamlessly repaired by utilizing the target plasmid as a template.

Why Work With Us

Advanced Technology Platform
Our service utilizes cutting-edge technology for C. elegans MosSCI transgenesis. We employ state-of-the-art techniques to ensure efficient and precise transgene integration.

Experienced Team of Experts
Our service is supported by a team of highly skilled and knowledgeable experts with extensive experience in C. elegans research and transgenesis methodologies.

Comprehensive Technical Traceability
We provide a whole-process technical trace service, ensuring transparency and accountability. Detailed documentation and records are maintained throughout the transgenesis process for reference and quality assurance.

Short Turnaround Time
We understand the importance of time efficiency and offer a shorter turnaround time for our services.

Workflow of C. elegans MosSCI service

We have developed a systematic platform for transgenesis in Caenorhabditis elegans using the MosSCI method, and the workflow is shown below.

  1. Determine the genomic target site for transgene insertion.
  2. Select Mos1 Alleles and Verify the presence of Mos1 transposase

  3. Plasmid Construction of transgene expression.

  4. Plasmid Microinjection

  5. Screening for transgenic animals

  6. Confirmation of transgene integration

  7. Delivering the C. elegans model and validation report

C. elegans

MosSCI Service

The MosSCI service enables you to integrate your desired sequence as a single copy insertion at a designated locus in the C. elegans genome. This is advantageous as it allows you to leave the native gene unmodified. With the MosSCI service you can:

  • Integrate a humanized gene into the C. elegans genome
  • Integrate your gene of interest into the genome with a fluorescent tag
  • Integrate your gene of interest into the genome with an immunotag
  • Examine your gene of interest’s expression under the control of a different promoter

C. elegans MosSCI Service Pricing

Service Package

Price

Est. Delivery Time

Full Build

$4,635

8 weeks and up

Candidate Lines

$2,785

6 weeks and up

Custom Injection Mix

$1,620

4 weeks and up

Note: Additional charge applies for cargo over 4.5kb in size for all packages.
 

Other Gene Editing Service We Provide

CRISPR C. Elegans Custom Injection Service

C. Elegans Extrachromosomal Array Service

Zebrafish Genome Editing Service

MosSCI Publications

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