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View from the Lab

A View From the Bench: Roadblocks and Frustrations When Expectation Meets Reality.

In an effort to create better models for epilepsy, we’ve been working on a grant-funded project to humanize important synaptic machinery by replacing endogenous coding sequences with human coding sequences.  Syntaxin is a key synaptic protein, encoded by the gene STX1A, that helps synaptic vesicles dock, fuse with the membrane, and release neurotransmitters.  Syntaxin has …

A View From the Bench: Roadblocks and Frustrations When Expectation Meets Reality. Read More »

Why Validating Your Plasmids is Key to a Successful Transgenic, and How You Can Do It Too.

Plasmids are a key tool in molecular biology. These circular pieces of DNA, also called “vectors” or “constructs”,  can be engineered to contain large made-to-order stretches of synthetic DNA. We design plasmids when we need to insert large stretches of synthetic DNA into the genome to create a model organism expressing, for instance,  a fluorescent …

Why Validating Your Plasmids is Key to a Successful Transgenic, and How You Can Do It Too. Read More »

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