Cat No. DYE9440

RediStain™ WormDye Lyso

$135.00

Shipping Details:

– USA: $15 flat rate via USPS or use your own shipper account.
– International: $120 or use your own shipping account.

Product Details

RediStain™ WormDye Lyso is a vital stain that can be used to stain apoptotic corpses in the C. elegans gonad in live worms. It is also used as a lysosomal marker.

Product Information:

  • Size: 500 uL (100 uses)
  • Packaged Concentration: 4 mg/mL
  • Storage upon receipt: <-20℃. Protect from light.
  • Solute: water
  • Excitation: 460 (RNA), 500 (DNA)
  • Emission: 650 (RNA), 526 (DNA)

Lysosomes and apoptotic corpses visualized using RediStainTM WormDye Lyso, which fluoresces red (549/565 excitation/emission). Young adult imaged after 30-min incubation in dye; head is on the right.

Documents

Protocol (PDF)

Safety Data Sheet (PDF)

Certificate of Analysis

Lot number 51191.pdf

 

References

  1. Hersh, B. M., Hartwig, E., and Horvitz, H. R. (2002). The Caenorhabditis elegans mucolipin-like gene cup-5 is essential for viability and regulates lysosomes in multiple cell types. Proc. Natl. Acad. Sci. USA 99, 4355–4360.
  2. Clokey, G. V., and Jacobson, L. A. (1986). The autofluorescent “lipofuscin granules” in the intestinal cells of Caenorhabditis elegans are secondary lysosomes. Mech. Ageing Dev. 35, 79–94.
  3. Kang J, Shin D, Yu JR, Lee J. (2009) Lats kinase is involved in the intestinal apical membrane integrity in the nematode Caenorhabditis elegans. Development. Aug;136(16):2705-15. doi: 10.1242/dev.035485.

 

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