We've compiled a collection of articles and reviews that discusses a variety of zebrafish genome editing methodologies, as well as our trials and errors on how to successfully deploy editing techniques.
Check out the infographic to learn more about the costs of DIYing your own zebrafish CRISPR project verses working with our team of experts.
We discuss the biggest pitfalls, pain points and precautions our team has encountered at the bench and in the zebrafish community so you can be fully prepared as you embark on your own knock-in odyssey.
We have overcome the challenge of generating mutant lines by focusing on the screening process, namely in substantial variability in PCR detection of loxP site integration at target loci.
In a recent publication, Dr. Tanguay and her lab created a new fluorescent-tagged zebrafish line to study the effects of Vitamin E on embryonic development. This article will give an overview of the Tanguay Lab’s work, and discuss: ‘why zebrafish’?
Successful zebrafish knock-ins are hard, we are not going to sugar coat it for you, but we at InVivo Biosystems are approaching an 80% success rate in our knock-ins and wanted to share with you what we believe to be the 6 key steps to having a successful knock-in project.
Zebrafish researchers have historically been plagued by a paucity of robust antibodies in zebrafish. One of our zebrafish experts explains why the advent of CRISPR/Cas9-mediated gene editing in zebrafish makes it possible to circumvent this problem.
The recent advancements of CRISPR and next-generation technology has enabled researchers to create more precise zebrafish models of human disease. This being said, knock-in (KI) techniques in zebrafish still aren’t fully optimized. In this article we discuss the current state of CRISPR-Cas9-mediated targeted knock-ins in zebrafish, and what the future holds.
At InVivo Biosystems, we specialize in multiple different ways of zebrafish genome editing such as an insertion, deletion, or replacement of DNA at a specific site in a genome, which allows us use the zebrafish to study detailed characteristics of its human ortholog. In this article we will discuss and compare the tools we use to perform our genome editing, CRISPR and Tol2.
Although there have been literature reports for years, the number of labs that struggle and fail with knock-ins far outweigh the number that find success and publish. So why do so many zebrafish groups struggle to achieve knock-in transgenesis? What is so hard about knock-ins? To start to understand, let's talk a little more about CRISPR, the technique that makes knock-ins possible.
Zebrafish are emerging as a valuable animal model for CRISPR work, including the determination of off-target effects.