NemaGel is a temperature-dependent (temperature is adjusted to 25°C) and reversible immobilization solution for imaging live C. elegans, immobilization occurs immediately upon temperature adjustment with NemaGel.
NemaGel uses polystyrene microbeads to allow for a uniform pressure on the coverslip without damaging the C. elegans. The uniform pressure allows for more complete compression and immobilization of the worm sample in the gel.
NemaGel will form gels above 10°C and re-liquefy when cooled to below 10°C. The gel should remain stable during autoclaving.
NemaGel is provided in an easy to use dropper bottle with simple protocols.
Size: 10ml/dropper bottle. For 20 uses. All orders are packaged in individual dropper bottle.
Minimum order is 2 vials (20ml for 40 uses).
Shipping: Room temperature.
Storage: Store at 2°C to 6°C upon arrival.
Use: Allow 1-4 hours for the gel to become liquid prior to the first use. Shake before use. Use directly.
Slide Preparation and Image Collection
- Add 10ul of M9 to the center of a slide.
- Pick C. elegans to the M9 drop (Alternative, use a suspended solution of worms)
- Surround M9 with cooled (4°C) NemaGel solution. Click to view.
- Add 1 drop of NemaGel to the center of a coverslip, then apply to the center of the slide.
- Heat the slide to 25°C and image.
- Remove the coverslip from the slide. Rinse both the coverslip and slide with 30ul M9 onto an agar plate.
- C. elegans will slowly recover movement (fully mobile in 4 minutes).
Tips When Using NemaGel
- Work quickly when applying the NemaGel as it solidifies rapidly (~30s) at room temperature.
- You can use a bead water bath or temperature block to obtain 25°C immobilization.
- For some imaging purposes, room temperature may provide sufficient immobilization.