Nema Imager

Visualize C. elegans behaviors and capture high frame-rate video with image quality comparable to traditional  inverted microscopes, suitable for calcium imaging.

Video: Visualize worm movement. Image captured using the Nema Imager.

Video: Fluorescent worm image captured using the Nema Imager

The Nema Imager is a spacing-saving and versatile imaging station. It's easy to use and more affordable compared to a traditional inverted microscopes. You can study a variety of C. elegans behaviors including: reward behaviors, social behaviors, avoidance behaviors, sickness behaviors, stress behaviors, and etc. It’s best suited for:

  • Easy integration into optogenetic and calcium imaging experiments
  • Live imaging and high frame-rate recording of freely moving worms in microfluidic devices.
  • High-quality images of worms immobilized on agar pads, with glue, or in hydrogel.
  • Imaging of fluorophores in the green family (GFP, GCaMP, etc.), configurable to provide three color detection of blue, green and red fluorophores.
  • Phase contrast images of internal structures.

To scale, the Nema Imager (right) next to a traditional inverted microscope (left).

Key Features:

• Low and high power, long working distance objectives allow for imaging of small structures.

• The large microscope deck is tapped with ¼-20 thru holes for maximum versatility to mount additional accessories.

• 2.2 MPix, 2/3” CMOS USB 3 camera. monochrome default, color and near-infrared models available.

• Fits RMS threaded objectives

• Three color model includes:
- Channel 1: Excitation 370-410 nm; Emission 429-462 nm
- Channel 2: Excitation 473-491 nm; Emission 502-561 nm
- Channel 3: Excitation 580-598 nm; Emission 612-680 nm

Base Model Product Specifications:

  • Optics: Green fluorescence; Phase contrast / brightfield (transmitted light)
  • Phase Contrast: Phase Contrast Accessory
  •  Objective Options: 10x
  • Objective Compatibilities: RMS-threaded, infinity corrected, 45 mm parfocal distance
  • Fluorescence Filters: 1 Channel: Green - Excitation 457-493 nm; Emission 508-552 nm
  • Camera: 125Hz, High Sensitivity Monochrome (B&W); C-mount
  • Image Formats: BMP, JPG, PNG, or TIF
  • Computer Requirements: Windows 10 and up
  • Dimensions (WxDxH):
    - 24 cm W x 17.5 cm D x 16.5 cm H (9.4 in W x 6.9 in D x 6.5 in H)
    - Incl phase: 24 cm W x 23 cm D x 37.8 cm H (9.4 in W x 9.1 in D x 14.9 in H)
  • Weight: 3.25 kg (7.1 lb); without phase; 4.5 kg (10 lb) incl phase
  • Operating Conditions: 0°C - 42°C, 5% - 95% RH non-condensing
  • Warranty: 1 year parts & labor
Nema Imager

Add-on options:

  • Fluorescence Filters (2 Channels): NOT upgradable after purchase
    - Excitation 370-410 nm; Emission 429 - 462 nm
    - Excitation 580-598 nm; Emission 612 - 680 nm
  • Objective Options: 4x, 10x 20x, 40x, 40x-LWD, 63x, 63x-LWD, 100x
  • Camera: 125Hz, High Sensitivity color; C-mount
  • Channel Rhodopsine stimulation: Blue light pulse

Ordering Info:

ProductCat. No.UnitPrice
Nema Imager 2 Channel BW NIM200A1
Nema Imager 2 Channel Clr NIM200B1$18,500
Nema Imager 4 Channels BWNIM400A1$34,000
Nema Imager 4 Channels ClrNIM400B1$34,000
Objectives: 4x, 10x, 20x, 40x, 40x-LWD, 63x, 63x-LWD, 100x1Request a quote

Looking for a more affordable alternative? Check out the Etaluma LS560 Microscope.

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