Nema Imager

Visualize C. elegans behaviors and capture high frame-rate video with image quality comparable to traditional inverted microscopes, suitable for calcium imaging.

Video: Visualize worm movement. Image captured using the Nema Imager.

Video: Fluorescent worm image captured using the Nema Imager

The Nema Imager is a spacing-saving and versatile imaging station. It's easy to use and more affordable compared to a traditional inverted microscopes. You can study a variety of C. elegans behaviors including: reward behaviors, social behaviors, avoidance behaviors, sickness behaviors, stress behaviors, and etc. It’s best suited for:

Easy integration into optogenetic and calcium imaging experiments
Live imaging and high frame-rate recording of freely moving worms in microfluidic devices.
High-quality images of worms immobilized on agar pads, with glue, or in hydrogel.
Imaging of fluorophores in the green family (GFP, GCaMP, etc.), configurable to provide three color detection of blue, green and red fluorophores.
Phase contrast images of internal structures.

To scale, the Nema Imager (right) next to a traditional inverted microscope (left).

Key Features:

• Low and high power, long working distance objectives allow for imaging of small structures.

• The large microscope deck is tapped with ¼-20 thru holes for maximum versatility to mount additional accessories.

• 2.2 MPix, 2/3” CMOS USB 3 camera. monochrome default, color and near-infrared models available.

• Fits RMS threaded objectives

• Three color model includes:
- Channel 1: Excitation 370-410 nm; Emission 429-462 nm
- Channel 2: Excitation 473-491 nm; Emission 502-561 nm
- Channel 3: Excitation 580-598 nm; Emission 612-680 nm

Base Model Product Specifications:

Optics: Green fluorescence; Phase contrast / brightfield (transmitted light)
Phase Contrast: Phase Contrast Accessory
Objective Options: 10x
Objective Compatibilities: RMS-threaded, infinity corrected, 45 mm parfocal distance
Fluorescence Filters: 1 Channel: Green - Excitation 457-493 nm; Emission 508-552 nm
Camera: 125Hz, High Sensitivity Monochrome (B&W); C-mount
Image Formats: BMP, JPG, PNG, or TIF
Computer Requirements: Windows 10 and up
Dimensions (WxDxH):
- 24 cm W x 17.5 cm D x 16.5 cm H (9.4 in W x 6.9 in D x 6.5 in H)
- Incl phase: 24 cm W x 23 cm D x 37.8 cm H (9.4 in W x 9.1 in D x 14.9 in H)
Weight: 3.25 kg (7.1 lb); without phase; 4.5 kg (10 lb) incl phase
Operating Conditions: 0°C - 42°C, 5% - 95% RH non-condensing
Warranty: 1 year parts & labor

Add-on options:

Fluorescence Filters (2 Channels): NOT upgradable after purchase
- Excitation 370-410 nm; Emission 429 - 462 nm
- Excitation 580-598 nm; Emission 612 - 680 nm
Objective Options: 4x, 10x 20x, 40x, 40x-LWD, 63x, 63x-LWD, 100x
Camera: 125Hz, High Sensitivity color; C-mount
Channel Rhodopsine stimulation: Blue light pulse

Ordering Info:

Product	Cat. No.	Unit	Price
Nema Imager 2 Channel BW	NIM200A	1	$18,500
Nema Imager 2 Channel Clr	NIM200B	1	$18,500
Nema Imager 4 Channels BW	NIM400A	1	$34,000
Nema Imager 4 Channels Clr	NIM400B	1	$34,000
Objectives: 4x, 10x, 20x, 40x, 40x-LWD, 63x, 63x-LWD, 100x		1	Request a quote

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Looking for a more affordable alternative? Check out the Etaluma LS560 Microscope.