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Preclinical Drug Discovery & Development with Zebrafish

InVivo Biosystem is your gateway to cutting-edge Comprehensive Zebrafish CRO Services, empowering researchers to unlock the full potential of zebrafish as a powerful model organism. Our seasoned team of zebrafish specialists is equipped with state-of-the-art technologies and expertise to provide you with a wide range of services, including genetic manipulation, behavioral studies, high-throughput screening, and more.

Our Zebrafish CRO Services

We offer precise and targeted gene editing, enabling you to create zebrafish lines tailor-made for your specific research needs. By harnessing the capabilities of zebrafish in combination with rodent models, you gain complementary data sets that lead to groundbreaking discoveries in biomedical research.

Expert in zebrafish research | InVivo Biosystems

Therapeutics Testing & Evaluation

– Generation of disease models (either chemically or genetically) in order to determine if your lead compound(s) ameliorate disease phenotypes or affect important metabolites.
Disease Model Creation | InVivo Biosystems

Disease Model Creation

— Genetically modified —

— Knockout

— Knock-in

— Point mutation

— Humanized

— Chemical models —

Therapeutic Testing | InVivo Biosystems

Therapeutic Testing

— Small Molecule

— ASO

— mRNA

— Biologics

— Library Screening

Disease Relevant Endpoints Analysis

Disease Relevant
Endpoints Analysis

— Phenotypic

— Molecular

— Protein Analysis

— Imaging

— Gene Expression

— Bioavailability

Genetic Target Validation & Discovery

– For Crispant screening, we use CRISPR to generate transient KOs (termed F0 crispants) for a gene of interest. This can be used to rapidly determine whether genes are involved in disease processes and make good targets for further discovery.

Disease Model Creation | InVivo Biosystems

Crispants/ Disrupt Genes

—- Create knockouts in the first generation

—- Disrupt 10-100’s genes

Therapeutic Testing | InVivo Biosystems

Screen Disease
Relevant Endpoints

— Phenotypic

— Molecular

— Protein Analysis

— Imaging

— Gene Expression

Disease Relevant Endpoints Analysis

Identify & Confirm Hits

— Discover which genes are disease relevant

Library & Candidate Screening

– Screen compound libraries and biologics (up to BSL2) for effects on a variety of readouts, including gene expression, fluorescence, and morphometrics.

Disease Model Creation | InVivo Biosystems

Disease Model Creation

— Genetically modified —

— Knockout

— Knock-in

— Point mutation

— Humanized

— Chemical models —

Therapeutic Testing | InVivo Biosystems

Library Screening

— Targeted

— Repurposed drug library

Disease Relevant Endpoints Analysis

Disease Relevant
Endpoints Analysis

— Phenotypic

— Molecular

— Protein Analysis

— Imaging

— Gene Expression

— Bioavailability

ADME-Tox and Acute Toxicity Testing

– ADME-Tox (Absorption, Distribution, Metabolism, Excretion – Toxicology) involves tissue preparation for ADME analysis. Our Acute Embryo Toxicity testing meets OECD 236 guidelines. We also offer targeted hepatotoxicity, neurotoxicity, and cardiotoxicity analysis.

— Test a range of concentrations, we define concentrations such as the LD50, NOEL, and MNLD.ing.

— Assess Morphological endpoints and Behavioral responses.

— Perform gene expression studies to characterize mechanisms of action.

— Provide toxicity response data in developing zebrafish at a whole transcriptome level.

Other Toxicity Testing

Neurotoxicity Testing

— Hepatotoxicity Testing

— Cardiotoxicity Testing

Our Core Capabilities

As an expert in zebrafish research, our core competencies reside in the following areas:

CRISPR genome editing

Deep Phenotyping

Omics-based analysis

Our phenotype-based drug discovery process utilizes zebrafish as an in vivo model to bridge the gap between cell culture and murine models. This approach allows target-independent drug discovery and emphasizes ameliorating phenotypes. As a result, efficacious drugs can be identified more quickly and cost-effectively. The zebrafish model has become highly translational due to advances in genetics, enabling reliable expression of human proteins. Our services are suitable for various projects, including:

  • Finding alternative therapeutic indications (aka indication expansion) for your compounds
  • Discovering new therapeutic applications for an existing drug target
  • Discovering novel therapeutics to assist in the treatment of a disease
  • Vetting drug targets identified in GWAS studies

Our Process

InVivo Biosystems Process #1

Introductory Call

We spend time getting to know your particular goals and specific testing needs. We advise you on whether our service is a good fit for your project.

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InVivo Biosystems Process #2
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Design & Planning

Our team of experts go to work designing your custom project and will provide the design within 2 weeks. Our deep knowledge and experience means that we can tackle difficult genetic design problems successfully so that you can get the data you need quickly.

InVivo Biosystems Process #3

Pilot Project

For new clients and new models, we run a custom pilot project. Pilot projects are quick and limited in scope, so that you can be confident that our models and assays will yield useful pre-clinical data.

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InVivo Biosystems Process #4

Comprehensive Project

After approval of a successful pilot project, we undertake the complete project. We provide you with:
• Direct contact with your project manager
• Bi-weekly updates on project status
• A final report including comprehensive data analysis and key take aways

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Take advantage of our Zebrafish Knock-In service today and unlock new possibilities for your drug discovery and development projects. Get in touch with us today and tap into our comprehensive services to revolutionize the landscape of healthcare.