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The Challenge of Getting CRISPR-based Knock-ins to Work in Zebrafish

Just the other day, I was talking with a collaborator who told me that they didn’t really believe that knock-ins really worked in zebrafish. Although there have been literature reports for years, the number of labs that struggle and fail with this technique far outweigh the number that find success and publish. So I can …

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6 things that can go wrong while making your Zebrafish CRISPR Knock-In

With the advent of targeted nucleases like zinc-finger nucleases (ZFNs), TALE nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas), zebrafish researchers were able to begin interrogating gene function and regions of interest with a precision that was previously out of reach. With these innovations, researchers could not only disrupt …

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Seventeen Minutes of Science: Ben Loves Zebrafish & CRISPR (Episode 1)

Stuck at home with nothing to do during this quarantine? Restless to still be learning and interacting with other scientists? You’re in luck! We are excited to announce our new virtual series, “Seventeen Minutes of Science”. Every Tuesday at 11am PST/ 2pm ET we will go live on Facebook to talk about science for (you …

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To truly validate your sgRNAs, go In Vivo

CRISPR/Cas9 gene editing for Zebrafish is a rapidly growing molecular biology toolset with diverse applications, but is not without its technological barriers. Reagent sourcing, design and validation of cutting efficiency, and donor homology design are challenging, lengthy processes without guarantee of success even for highly skilled researchers. One way to enable researchers to initiate a …

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Zebrafish and C. elegans in ALS: tightening the bench-to-clinic gap

While advances in molecular imaging, high-throughput screening, genomics and techniques like CRISPR-Cas9 gene editing have dramatically enhanced our understanding of the human body and disease, the pace of advancing new treatments for those diseases has lagged behind significantly. One way to shorten the time between laboratory discoveries and available new drugs is to use animal …

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