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C. elegans

A View From the Bench: Roadblocks and Frustrations When Expectation Meets Reality.

In an effort to create better models for epilepsy, we’ve been working on a grant-funded project to humanize important synaptic machinery by replacing endogenous coding sequences with human coding sequences.  Syntaxin is a key synaptic protein, encoded by the gene STX1A, that helps synaptic vesicles dock, fuse with the membrane, and release neurotransmitters.  Syntaxin has …

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We Used CRISPR to Make 15 Point Mutations in 5 Genes. Here’s What We Learned.

You may or may not know that at InVivo Biosystems, we conduct a lot of grant funded research as well as offering custom models and assays to our clients. Much of our grant activity in the past few years has centered on creating and characterizing disease-associated variants and point mutations. We’ve created collaborations and partnerships …

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Why Validating Your Plasmids is Key to a Successful Transgenic, and How You Can Do It Too.

Plasmids are a key tool in molecular biology. These circular pieces of DNA, also called “vectors” or “constructs”,  can be engineered to contain large made-to-order stretches of synthetic DNA. We design plasmids when we need to insert large stretches of synthetic DNA into the genome to create a model organism expressing, for instance,  a fluorescent …

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Precise Knock-outs for Precise Research

Knockouts are the bread and butter of reverse genetic studies. They allow researchers to study the effects of the absence of a particular gene, protein segment, amino acid, or regulatory element, thereby enabling the researcher to determine their function. Many of the classically used and widely available knockout lines researchers rely on are not precise …

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5 Simple Steps to Improve Your Injection Success

We do injections everyday. While we’ve gotten pretty good at them, they don’t always go as planned. What does it mean if an injection has gone awry, and how can you tell? What does an unsuccessful injection look like? The two videos above illustrate what a successful injection (left) looks like, and what an unsuccessful …

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Seventeen Minutes of Science: Worming our way into Machine Learning (Episode 9)

Stuck at home with nothing to do during this quarantine? Restless to still be learning and interacting with other scientists? You’re in luck! You can now tune in weekly to our virtual series “Seventeen Minutes of Science”. Every Tuesday at 11am PST / 2pm ET we will go live on Facebook to talk about science …

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