HomeLifespan & Healthspan Analysis using C. elegans

Lifespan & Healthspan Analysis using C. elegans

Our in-vivo lifespan and healthspan analysis offers 3 service packages:

  • Lifespan and healthspan analysis: produce lifespan and healthspan measurements by automatically monitoring an adult C. elegans’ survival, morphology and movement using the data collected over the entire lifespan of the adult C. elegans. 
  • Pathway analysis: identify the pathways that contribute to the observed lifespan extension using RNAseq or whole transcriptome sequencing (WTS).
  • Compound testing: determine a compound’s ability to extend lifespan and healthspan within 4 months once an experiment starts.

Lifespan & Healthspan Analysis

We can simultaneously measure the lifespan and healthspan by monitoring an adult C. elegans’ survival, morphology and movement and produce a comprehensive analysis (7 different healthspan readings on the same worms) using the data automatically collected over the entire lifespan of the adult C. elegans.

Lifespan assay 2

Fig. 1: Aggregate movement analysis over duration of lifespan. Black line: Survival Green line: Aggregate mean displacement of worms on the plate. A single trace from one condition is shown for demonstration.

Lifespan assay 1

Fig. 2: Survival and hazard curves from lifespan assay. (A) Survival curves for 10μM Lu0128 (blue), vehicle control (green), or positive control 50μM NAC (orange). (B) Cumulative hazard plots indicating the risk of death over time.

Pathway Analysis

We can identify the pathways that contribute to the observed lifespan extension using RNA-Seq or whole transcriptome sequencing (WTS). For example, we can identify the anti-aging pathways that contribute to the observed lifespan extension from the lifespan analysis.

Change-in-gene-expression

Fig. 3: Measure changes in gene expression level. The gene asp-12 is downregulated 117-fold (p=1.3e-37) in the day 10 Lu0128 treated samples compared with the day 10 untreated. The gene lea-1 is upregulated 6.5-fold (p=2.4e-16) in the day 10 Lu0128 treated samples compared with the day 10 untreated.

Fig. 4: Aging pathway analysis. Global gene expression analysis indicates two candidate pathways likely to mediate the effect of compound on lifespan: oxidative phosphorylation and oxidative stress.

Compound Testing

Our compound testing platform is an effective approach to test the effects of a compound on lifespan, healthspan, and transcriptional changes related to aging. Using C. elegans, our 3-step approach provides insights into the mechanism of action for lifespan and healthspan.

  • Step I: Conduct a dose and toxicity assessment of the compound to determine whether a full longevity experiment is feasible and at what dosage (Fig. 5).
  • Step II: Measure the lifespan and vitality extending effects of the compound by monitoring the adult C. elegans’ survival, morphology and movement. The healthspan metrics are extracted from the same dataset as the lifespan readings (Fig. 6).
  • Step III: Identify the aging pathways that contribute to the observed lifespan extension from Step II (Fig. 3 & 4 above).
dose-response-curve

Fig. 5: Dose-response curve to determine the stress level introduced by the compound. Day 1 adult worms were treated with Lu0128 at 1µM, 10µM, 100µM, 1mM, and 10mM for 12 hours.  A) Xenobiotic stress biosensor response assay. B) Oxidative stress response assay. Calculated working EC50 = 21µM.

Compound testing set-copy_2

Fig. 6: Assessing the eligibility of 2 promising compounds. (A) Compound A increases lifespan (orange) compared to untreated control. (B) In addition, exposure to compound A triggers a considerable improvement of healthspan, with 20-day old worms exposed to compound A exhibiting an overall activity level similar to untreated worms (grey) that are only 12-day old. Unlike control populations, worms exposed to compound A do not exhibits complete loss of activity prior to death. (C) Compound B also shows a strong longevity effect (purple), but (D) triggers a sharp, early decline in healthspan leading to a complete loss of locomotion by Day 23.

Our Process

Introductions
Understand your goal

We’ll set up a 30-minute call with you to understand your needs. During this call, we will define the scope of your project, answer your practical questions and help you assess whether our service is a good fit for you.

Design
Create a personalized catalog 

After we decided on the scope of the project, we’ll create a proposal that includes a time and cost estimate for each experiments that we propose. You will be able to personalize your project plan with us.

Logistics
Finalize your project design

We’ll send you a final statement of work and payment schedule. Once we receive your first PO, we will start the experiments and give you an overview of the timeline of your project.

Report Out
Maintain clarity and transparency

We’ll keep you updated every two weeks on the status of your project. At the end of your project, we provide you with a comprehensive report that includes findings and key takeaways.

Ready to Get Started?

Ready to connect with us to learn more about working with us on compound testing?

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