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wMicroTracker

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Introducing wMicroTracker SMART

Healthspan and Lifespan Experiments in 5 Minutes Each Day

With wMicroTracker SMART, you can now perform healthspan and lifespan experiments at once by measuring each plate only 5 minutes each day. It is quantitative with no human bias.

Keep the plates in the incubator. When you are ready, take the plate out of the incubator, place it in the tray and measure it for 5 minutes. Yes, it is that simple – 5 minutes each day to get reliable and reproducible healthspan and lifespan data. Best of all, wMicrotracker SMART is small and light for your budget!

With wMicroTracker SMART and ONE, now you can measure small animal movements and population behavior quantitatively as well as perform non-invasive phenotyping.

wMicrotracker-Smart

wMicroTracker SMART vs. ONE

wMicrotracker SMART

wMicrotracker ONE

Liquid Culture

High content

High throughput

Technology

infrared whole plate imaging

infrared microbeam light scattering

Readout output

speed, travel distance, motility index, position, overall activity

overall activity

Speed capacity

12 experiments per hour [1 x plate / 5m]

192 experiments per hour [1 x 96well plate / 30m]

Acquisition time

5 minutes

30 minutes

Plate format compatibility

35mm Petri dish

best for 96 well microplate Also compatible with: 384, 24, 6 well plates

Recommended for

NGM Culture
Secondary screening

Liquid culture
Fast Primary screening
High number of samples to test

Minimum Organism size (C. elegans)

> 0.8 mm (L4 stage or bigger)

> 0.1mm (L1 stage or bigger) Good swimmers. Non-uncs

No. of animals per well (C. elegans)

15 to 35

25 to 50

Cross-compatibility [validated]

15 to 35

parasitic nematodes* Zebrafish larvae

Drug/RNAi/mutant Screenings

Yes

Yes

Toxicity / anthelmintic

Yes

Yes

Antioxidant

Yes

Yes

RESEARCH APPLICATION

Microbiome

Yes

Likely

COMPATIBILITY

Chemotaxis / Memory

Yes

No

detailed single animal posture

No

No

Healthspan

Yes

Yes

Lifespan

Yes

No

wMicroTracker

24/7 Automated Data Collection

The wMicroTracker measures overall locomotor activity and viability of your worms such as C. elegans and parasitic nematodes cultured in liquid media and in multi-well plates. Its nonlabor-intensive automated assay is capable of providing a simple overnight readout which requires no additional processing.

This system is optimized for measuring C. elegans in liquid media and is also designed for high-throughput screening assays to evaluate the biological effects of potential compounds/drugs, as well as for mutant phenotyping studies in small nematodes.

With the wMicroTracker’s 24/7 automated data collection capability, you can:

The wMicroTracker gives you data comparable to a thrashing assay, only 10 times faster

The wMicroTracker quantifies the locomotor activity of a worm population in a 96 well plate with 30-70 animals per well. Data acquisition and analysis are automated and simultaneous. All activity is normalized to N2 controls.

Toxicity Assay

The dose-response effect of oxidative stress on C. elegans locomotor activity over time can be observed. Worms were exposed to a reactive oxygen species (ROS) generating compound. Each concentration was tested four times, using 30-70 animals per well in a 96-well plate.

Lifespan Assay

The decreased lifespan of skn-1 mutants can be observed. Samples were recorded for 1 hour once per day, four times per condition.

Using wMicroTracker ONE for Parasitic Nematodes

Measuring Locomotor Activity

The wMicroTracker ONE instrument can be used to measure the locomotor activity of parasitic nematodes cultured in liquid media and in multi-well plates. Users can get measurements of overall locomotor activity and viability of worms of various species and sizes.

Key advantages:

Figure 4. Detect and measure E. granulosus protoscoleces movement using the WMicroTracker System. Movement is detected when a population of more than 45 organisms/well is employed. Linear range is observed from 50 to 200 organisms/well for a 30-minute observation period.

 

Figure 2. The concentration-response curve of EVP4593 on four ruminant parasites (mean ± S.D., n = 3). (Liu et al. 2018)

Measuring Anthelmintic Activity

The conventional methods of measuring anthelmintic efficiency on parasitic nematode larvae, are image-based, which makes them low throughput and often laborious. The wMicroTracker instrument can be used for library screening and drug discovery in a variety of parasitic nematodes.

Species Compatibility

The conventional way of measuring anthelmintic activity for parasitic nematodes are laborious and low throughput. The wMicroTracker instrument can be used for library screening and drug discovery in parasitic nematodes.

The wMicroTracker has been tested and validated for the following parasitic species:

Learn more about the species compatibility by downloading the table below.

WMicrotracker Features

Liquid Culture

Works with 96 well microplates (Greiner)

Fits in an incubator (for temp. control)

Toxicity Assays

Acute drug exposure

Stress Response

Requires multiple worms/plate or well

Temperature monitoring

Transgenics in Parasitic Nematodes

It is difficult and expensive to rear parasites due to hosts. The availability of homologs and paralogs of genes in a tractable genetic model such as C. elegans is one of the major contributions to parasitology. Transgenic C. elegans can be used as a method for studying drug resistance genes of parasitic nematodes. C. elegans is easy to grow in the lab, and there are lots of phenotypic assays available that are not yet available for parasitic nematode.

“CRISPR/Cas9 can be used to introduce nematode parasite genes into the experimentally tractable and anatomically and physiologically relevant C. elegans model system. Studies have shown that C. elegans knock‐out strains can be functionally rescued with transformation of parasite transgenes”. Zamanian & Anderson.

We can express parasite genes that are suitable for study in C. elegans. Share with us the parasitic worm gene you would like to study in C. elegans, and we would be happy to provide a no-cost analysis of project feasibility. We have successfully took sequence from the parasite strains including Loa loa, Dirofilaria immitis, Toxocara canis and inserted a functional gene in the C. elegans genome with MosSCI technology.

Product

Cat. No.

Price

wMicroTracker (PC included)

MTK100

$17,600

wMicroTracker ONE Publications

Measuring Activities using wMicroTracker:

Twitchin kinase inhibits muscle activity.

Matsunaga Y ; Hwang H ; Franke B ; Williams R ; Penley M ; Qadota H ; Yi H ; Morran LT ; Lu H ; Mayans O ; Benian GM. Mol Biol Cell. 2017 Jun 15;28(12):1591-1600.

Study of parasitic nematodes using C. elegans:

FAQs

Frequently Asked Questions

Using wMicrotracker, it is possible to perform experiments with up to 70 worms and as few as 1 worm.

The wMicroTracker can be used with standard “U” bottom and “flat” bottom microplates. The U-plate motion signals saturate more quickly than in flat bottom plates. We have also found the detection sensitivity to be greater in the “U” bottom plate. Not that for smaller populations, “U” bottom plates have less variability between wells.

Few number of worms are required, and subtle movements can be detected at the expense of smaller volumes of reagents.

Worm interactions are minimized in this case and signal linearity is maintained when the number of worms are increased or decreased.

The system is compatible with most worm strains including wild nematode isolates and parasitic nematodes. Non motile/non-good swimming strains (such as roll or severe unc mutants) are not recommended.

Yes, the Microtracker can measure worm activity form L1 to older adult for weeks. Be sure to validate your liquid culture protocol before using it in the WMicroTracker.

Although the wMicroTracker does not have a camera, it is possible to correlate the wMicroTracker’s data (number of beam breaks) to the number of body bends per minute.

You can measure movement in populations of larvae as early as L1.

It is not recommended. However, it is possible to perform experiments with as few as 1 worm. See graphs in this Tech Note for more details.

Yes. Worms can be measured in the wMicroTracker for multiple days when placed in bacteria. We recommend that you use fresh bacteria at an OD600 of 0.5 (up to 1). Under these conditions, adult worms usually have enough food for 3-5 days. Another option is to cultivate your worms in axenic media (CeMM) instead of using bacteria.

The wMicroTracker can acquire data on your worms for weeks without interruption. The limiting factor for longitudinal studies will be determined by your worm culture protocol and requirements. For long term data acquisition in the wMicroTracker, we recommend that you place your worms in axenic media. We recommend that you conduct measurements for at least 100 minutes to reduce standard error. See protocol for healthspan assays.

We observed no degradation of the LEDs over time. The LEDs in the wMicroTrackers have a lifetime of 36,000 hours. Since they flash every 1/384 sec, this is equivalent of 10 years of use. The wMicroTracker was engineered to auto-compensate and auto-calibrate the beams to maintain signal linearity over time.

The infra-red beams in the wMicroTracker are generated by low-power LEDs and have been shownto be non-invasive for C. elegans (Simonetta and Golombek 2007).

Yes, you can download the wMicroTracker software here.

The LED beams pulse every 1/384 sec and the software records every interruptions of the beams. This data is pooled and analyzed when the data report is generated by the software and presented as “average activity count by data interval”. You can always change the analysis bin (data interval) size after the data is collected. The minimum bin size recommended for analysis is 5 minutes. Choosing smaller bins may increase variability of your data.

The LED beams have a diameter of 150 µm, which is larger than the diameter of an adult C. elegans worm (100 µm on average).
What are the beam specs?
• Wavelength: Infrared, 880nm
• Temperature: no heat generated
• Pulse frequency: 1/384 sec
• Number of beams per well for the wMicroTracker.
– 96-well plate: 2 beams per well
– 384-well plate: 1 beam per well
These properties cannot be modified.

Computer minimum requirements:
• Pentium II processor or above (>1GHz clock)
• 512Mb of RAM memory
• 1 USB port
• Windows XP 32 bits (or higher) operating system
• 200Mb of free HD space (>10Gb free HD space recommended for real time data saving)

The wMicroTracker creates a folder for each experiment. The raw data for a 2-hour experiment is about 20 Kb. The Excel export file for such an experiment is about 15 Kb.

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