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Bridge the data gap between cell culture and rodent models. Rapidly conduct preclinical studies to de-risk your clinical trial.
An expert in CRISPR genome editing for creating custom genome edited zebrafish and C. elegans, our CRISPR genome editing platform enables preclinical studies and drug discovery by providing insights you need to derisk your clinical trial or decide the next phase of product devleopment.
An expert in CRISPR genome editing for creating custom genome edited zebrafish and C. elegans, our CRISPR genome editing platform enables preclinical studies and drug discovery by providing insights you need to derisk your clinical trial or decide the next phase of product devleopment.
Choose this option if you want a CRISPR knockin with completely validated reagents to use in your lab and to find your gene edited line. This is the best Custom Injection Mix option for the novice zebrafish line builder.
What’s Included?
Standard Injection Mix
Validated sgRNA Injection Mix
Fully Validated Injection Mix
Mosaic Clutch* (F0 injected embryos)
Full Build** (Sequence verified hetero zygous line)
KO Design
Injection Mix Assembly
Locus Evaluation & Sequencing Primers
in vivo sgRNA Testing
in vivo Editing Assessment & Screening Reagents
Expertly Injected Embryos
Germline Transmission Screening & Line Propagation
* This service is only available to clients in the United States.
** Full Build includes screening n=100 adults; standard husbandry charge applies.
# Includes evaluation of 2 sgRNAs
## Includes evaluation of 4 sgRNAs
We create a mix using quality custom reagents from our validated suppliers. The sgRNAs are duplexed with the Cas9 protein.
The mix is created using concentrations of Cas9, sgRNA and donor homology that work well in our genome editing pipeline. The mix is provided as a stable dehydrated reagent.
Four injection mixes are provided that reconstitute to 5ul of mix each.
You can miss your edit if you don’t have a good assay to detect it.
We will design and test a robust assay to detect your edit, which is critical when you begin germline screening.
We will also provide PCR primers and protocols for an edit detection assay.
Ensure that you have the quality reagents you need for a successful genome edit. The efficiency of the sgRNA guided cutting can vary widely and is directly tied to the achievement of a successful genome edit.
Algorithms and experience help us choose the best candidate sgRNAs for your project, but the guided cutting efficiency of an sgRNA can be impacted by genome organization, for example, chromatin accessibility. The best way to determine the effectiveness of an sgRNA in a Zebrafish genome is to test it in vivo in the injected embryos.
We test your sgRNAs for their ability to guide Cas9 cutting. Our process also identifies SNPs and polymorphoisms in your locus that may impact homology directed repair. Embryos are injected with two different sgRNA/Cas9 duplexes and we determine the cutting efficiency for each sgRNA. If both sgRNAs fail to guide successful cutting, a third sgRNA is injected and tested.
Additional sgRNA testing can also be added with consultation.
Have total peace of mind by having us test your injection mix in our facility.
Our skilled injectionists will inject embryos with your mix and determine the somatic integration efficiency by PCR.
We will confirm the precise edit by sequencing to ensure that your edit is occurring correctly with no erroneous insertions.
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An expert in CRISPR genome editing, InVivo Biosystems creates custom genome-edited C. elegans and zebrafish models to enable aging, developmental, and disease studies. Our unique in vivo platforms and technologies bridge the gap between cells and mice.
