Zebrafish Point Mutation Service
The Point Mutation service using zebrafish introduces a small number of nucleotide changes at a target site using CRISPR/Cas9. With this service you can:
- study a disease-causing mutation
- humanize a critical amino acid
- explore the binding site of an enzyme
- introduce phosphomimetics
- mutate isoform start sites or make any specific mutation of interest.
Precise Point Mutation of Stxbp1a in Zebrafish
Illustration of a successful precise point mutation of Stxbp1a in Zebrafish. Stxbp1a is a highly conserved zebrafish ortholog of human STXBP1 (87% identity). Using CRISPR/Cas9 technology, we were able to precisely generate a benign patient mutation at the conserved amino acid residue (CCC>CTG, p.P94L).
Service Offerings (price reflects academic pricing)
|Service Package||Price||Est. Delivery Time|
|Full Build||$26,955||9 - 12 months|
|Verified Clutch||$9,310||4 - 6 months|
|Custom Injection Mix (without verification of sgRNA cutting)||$995||4-6 weeks|
|Custom Injection Mix (with verification of sgRNA cutting)||$3,259||4 - 8 weeks|