Zebrafish Tol2 Transgenesis Services
Insert large content in the zebrafish genome using the Tol2 transposon-based system.
Using Tol2 transgenesis you can generate:
- Cre Recombinase-Driver lines
- Transcriptional reporters
- Models to analyze tissue-specific gene expression
- Models to control the timing of gene expression
- Humanized zebrafish
Comparison of Tol2 vs. CRISPR
| Nature of Edit | Number of Insertions | Germline Edits | Germline Efficiency | Common Terminology | Phenotype in F0 (injected embryos) | Max. Size of Insert | |
| Tol2 Transposase | |||||||
| CRISPR/Cas9 |
Tol2 Transgenesis Service Options
| Tol2 Plasmid Prep | Tol2 Plasmid Validation | Mosaic Clutch* (F0 injected embryos) | Full Build** (Stable transgenic line) | |
| Tol2 Plasmid Design |  | | | |
| Plasmid Assembly | | | | |
| InVivo Integration Assessment | | | |
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| Expertly Injected Embryos | | |
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| Germline Transmission Screening & Line Propagation | |
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| Pricing# | $2,995 and up | $5,595 and up | $7,595 and up | $21,840 and up |
| Timeline | 6 weeks and up | 3 months and up | 4 months and up | 9 months and up |
** Full Build includes screening n=100 adults; standard husbandry charge applies.
# Additional charge applies for cargo over 2kb in size for all service packages.
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