Zebrafish Tol2 Transgenesis Services
Insert large content in the zebrafish genome using the Tol2 transposon-based system.
Using Tol2 transgenesis you can generate:
- Cre Recombinase-Driver lines
- Transcriptional reporters
- Models to analyze tissue-specific gene expression
- Models to control the timing of gene expression
- Humanized zebrafish
Comparison of Tol2 vs. CRISPR
| Nature of Edit | Number of Insertions | Germline Edits | Germline Efficiency | Common Terminology | Phenotype in F0 (injected embryos) | Max. Size of Insert | |
| Tol2 Transposase | |||||||
| CRISPR/Cas9 |
Tol2 Transgenesis Service Options (price reflects academic pricing)
| Standard Injection Mix# | Complete Validated Injection Mix | Verified Clutch* (F0 injected Embryos) | Full Build** (Sequence verified heterozygous line) | |
| Tol2 Plasmid Design |  | | | |
| Injection Mix Assembly | | | | |
| InVivo Integration Assessment | | | |
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| Expertly Injected Embryos | | |
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| Germline Transmission Screening & Line Propagation | |
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| Pricing* | $2,995 | $5,595 | $7,595 | $21,840 |
| Timeline | 4-6 weeks | 4-8 weeks | 1-2 months | 4-6 months |
| More Details | More Details | More Details | More Details |
** Full Build includes screening n=100 adults; standard husbandry charge applies.
Additional charge applies for cargo over 2kb in size for all service packages.
Injection Mix and sgRNA Validation Details
Standard Injection Mix
Choose this option if you want us to pick your sgRNA site, have already tested your sgRNA in vivo, or want to make a CRISPR knockout. We send a custom designed injection mix and you develop your own preferred detection/screening protocol.
What’s Included?
- Consultation with a genetic engineer to develop the genome editing strategy
- In silico design of all reagents including the sgRNA site and donor homology template
- Sequence files for the donor homology construct and edited locus
- 4 vials of 10uL lyophilized sgRNAs duplexed with the Cas9 protein
- Injection dyes for early visualization of injection success
- Detailed Instructions on mix reconstitution and injection protocols.
Complete Validated Injection Mix
Choose this option if you want a CRISPR knockin with completely validated reagents to use in your lab and to find your gene edited line. This is the best Custom Injection Mix option for the novice zebrafish line builder.
What’s Included?
- OUR VALIDATED sgRNA INJECTION MIX PLUS:
- Full in vivo evaluation of somatic editing efficiency.
- Confirmation of integration capacity of your edit into the zebrafish genome
Verified Clutch (F0 injected Embryos)
Choose this option if you want a CRISPR knockin with completely validated reagents to use in your lab and to find your gene edited line. This is a good option for the researcher who wants to outsource part of the workload in the development of a new line.
What’s Included?
- Our Complete Validated Injection Mix PLUS:
- Expertly injected embryos for direct submission to you nursery
- Fully validated PCR primers and protocols for edit detection assay in your own lab.
Full Build (Sequence verified heterozygous line)
Choose this option if you want us to deliver a stable sequence validated CRISPR knockin line.
What’s Included?
- Veried Clutch (F0 injected Embryos) Plus:
- Growth of F0 injected embryos in our nursery
- Rigous screening of F0 injected adults for germline transmission of your edit
- Sequence conformation and propagation of F1 heterozygous animals
Injection Mix Service Component Descriptions
READY TO GET STARTED?
Ready to connect with us to learn more about working with our company or our technology?