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Zebrafish Tol2 Transgenesis Services

Zebrafish Tol2 Transgenesis Services

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Insert large content in the zebrafish genome using the Tol2 transposon-based system.

Using Tol2 transgenesis you can generate:

  • Cre Recombinase-Driver lines
  • Transcriptional reporters
  • Models to analyze tissue-specific gene expression
  • Models to control the timing of gene expression
  • Humanized zebrafish

Comparison of Tol2 vs. CRISPR

Nature of EditNumber of InsertionsGermline Edits
Germline EfficiencyCommon TerminologyPhenotype in F0 (injected embryos)Max. Size of Insert
Tol2 Transposase
Random insertion
Multiple
Yes
10% to 40%
Transgenesis
Mosaic
Usually 13-15kb - depends on cargo
CRISPR/Cas9
Site specific
Single
Yes
~2% to 5%
Knock-in Edit
Mosaic
Unknown but appears that the larger the insert the lower the efficiency.

Tol2 Transgenesis Service Options 

Tol2 Plasmid PrepTol2 Plasmid Validation Mosaic Clutch* (F0 injected embryos)Full Build** (Stable transgenic line)
Tol2 Plasmid Design
Plasmid Assembly
InVivo Integration Assessment
Expertly Injected Embryos
Germline Transmission
Screening & Line Propagation
Pricing#$2,995 and up$5,595 and up$7,595 and up$21,840 and up
Timeline6 weeks and up3 months and up4 months and up9 months and up
* This service is only available to clients in the United States.
** Full Build includes screening n=100 adults; standard husbandry charge applies.
# Additional charge applies for cargo over 2kb in size for all service packages.
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