C. elegans Mutation Packages

C. elegans Precise Gene Knockout Service

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Knockouts are the bread and butter of reverse genetic studies. They allow researchers to study the effects of the absence of a particular gene, protein segment, amino acid, or regulatory element, thereby enabling the researcher to determine their function.

Gene knockouts are a commonly used tool for biologists to understand gene function. Examination of phenotypes after gene deletion can reveal insights into the role of that gene in the organism.

Many of the classically used and widely available knockout lines researchers rely on are not precise deletions. Instead, many of these lines have an early stop inserted resulting in a partial gene product. They are truncations and not true deletions.

If your research requires a line with the deletion of a full gene we recommend working with a precise deletion to ensure the accuracy of your studies.

Using CRISPR/Cas9, InVivo Biosystems can delete the entire coding sequence of a gene. You can then study the phenotype of the true null allele with no concern that any protein function remains.

Our transgenic designers and process ensure that each knockout we make is exactly the right strain to answer the research question.

We have successfully deleted up to 43k bp and continue to test the limit. Any genomic region can be targeted for deletion.

Service Details (price reflects academic pricing)

Service PackagePriceEst. Delivery Time
Full Build $4,0756 - 8 Weeks
Candidate Lines $2,5543 - 4 Weeks
Custom Injection Mix $9951 - 2 Weeks

Knockout Publications

  • Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning & reduces neurodegeneration

    Veronica H. Ryan, Theodora Myrto Perdikari, Mandar T. Naik, Camillo F. Saueressig, Jeremy Lins, Gregory L. Dignon, Jeetain Mittal, Anne C. Hart, Nicolas L. Fawzi. bioRxiv. March 18, 2020.

  • Tensin regulates pharyngeal pumping in Caenorhabditis elegans

    Aaron N.Bruns, Su Hao Lo. Biochemical and Biophysical Research Communications
    Volume 522, Issue 3, 12 February 2020, Pages 599-603.

  • TRAIP drives replisome disassembly and mitotic DNA repair synthesis at sites of incomplete DNA replication

    Remi Sonneville, Rahul Bhowmick, Saskia Hoffmann, Niels Mailand, Ian D Hickson, Karim Labib. eLife 2019;8:e48686.

  • DOT1L complex suppresses transcription from enhancer elements and ectopic RNAi in Caenorhabditis elegans

    Esse R, Gushchanskaia ES, Lord A, Grishok, A. RNA. 2019.  

  • NEW FINDING A CRISPR/Cas9-generated cdc-7 loss of function mutation does not cause temperature-dependent fertility defects

    Heather Currey and Nicole Liachko. microPublication Biology, 2019.

  • Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity

    Woo JA, Liu T, Trotter C, Fang CC, De Narvaez E, LePochat P, Maslar D, Bukhari A, Zhao X, Deonarine A, Westerheide SD, Kang DE. Nat Commun. 2017 Jun 6;8:15558.

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