C. elegans Immunotag Service
The addition of an immunotag at the endogenous locus enables you to quantify your protein without altering the level of gene expression.
Immunotags are added using CRISPR/Cas9 gene editing technology. You can choose from a variety of tags including FLAG, HA, HIS, TAP, or S-peptide. All tags have been optimized for expression in C. elegans.
Process For Generating Strains
Transgenic Design
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Molecular Assembly
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Injections
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Candidate Screening
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Strain Confirmation
- Transgenic Design:
Design of donor homology template and guide sgRNAs for tagging of your gene of interest. - Molecular Assembly:
Construct high-quality components for genome editing including custom DNA donor homology templates, custom sgRNAs and validated Cas9 protein. - C. elegans Injections:
Our skilled injectionists perform microinjections of the protein, RNA, and DNA injection mix with high survival and transgenesis rates. - Candidate Screening:
Candidate animals containing the genomic integration of interest are initially identified via the co-CRISPR phenotype; confirm edit integration at the genomic level via molecular screening by PCR. - Strain Confirmation:
PCR testing to confirm that the lines have the desired construct integration and sequencing to confirm that the integration occurred without error.
Service Details (price reflects academic pricing)
| Service Package | Price | Est. Delivery Time |
|---|---|---|
| Full Build | $4,360 | 4 - 8 Weeks |
| Candidate Lines | $2,839 | 3 - 6 Weeks |
| Custom Injection Mix | $1,095 | 2 - 3 Weeks |
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