C. elegans Immunotag Service
The addition of an immunotag at the endogenous locus enables you to quantify your protein without altering the level of gene expression.
Immunotags are added using CRISPR/Cas9 gene editing technology. You can choose from a variety of tags including FLAG, HA, HIS, TAP, or S-peptide. All tags have been optimized for expression in C. elegans.
Process For Generating Strains
- Transgenic Design:
Design of donor homology template and guide sgRNAs for tagging of your gene of interest.
- Molecular Assembly:
Construct high-quality components for genome editing including custom DNA donor homology templates, custom sgRNAs and validated Cas9 protein.
- C. elegans Injections:
Our skilled injectionists perform microinjections of the protein, RNA, and DNA injection mix with high survival and transgenesis rates.
- Candidate Screening:
Candidate animals containing the genomic integration of interest are initially identified via the co-CRISPR phenotype; confirm edit integration at the genomic level via molecular screening by PCR.
- Strain Confirmation:
PCR testing to confirm that the lines have the desired construct integration and sequencing to confirm that the integration occurred without error.
Service Details (price reflects academic pricing)
|Service Package||Price||Est. Delivery Time|
|Full Build||$4,360||4 - 8 Weeks|
|Candidate Lines||$2,839||3 - 6 Weeks|
|Custom Injection Mix||$1,095||2 - 3 Weeks|