C. elegans Endogenous Tagging Packages

C. elegans Fluorescent Tag Service

showcase_fluorescent-tag

Fluorescent tagging of genes is widely used in many model organisms to study which tissues a gene of interest is expressed in, where in the cell a gene is expressed, or to determine whether multiple genes of interest are expressed in the same location. C. elegans is particularly well suited to fluorescence studies because they are transparent. Fluorescence can be easily observed in both live and fixed images.

Knock-in a fluorescent protein at the endogenous locus using CRISPR gene editing technology.

Adding a fluorescent protein tag at the endogenous locus enables you to:

  • detect your protein in-vivo at native gene expression levels
  • ensure that you have the correct expression pattern
  • identify protein-protein interactions utilizing splitGFP
  • confirm degradation of your protein when paired with a degron sequence
  • visualize protein synthesis rates
  • quantify protein levels

In the past 5 years, InVivo Biosystems has made over 600 fluorescent transgenic lines inclduing 15 different fluorophores, 8 variants of GFP. The image below is lmn-1 tagged endogenously with GFP showing a worm expressing our mCherry array markers, driven by the myo-2, myo-3 and rab-3 promoters.

Fluorescent Tagging. IIntestinal nuclei from late L4 worms in an lmn-1::GFP transgenic strain. Image courtesy of Dr. André Catic, Baylor College of Medicine.

Choose From a Variety of Proteins

You can choose from a variety of proteins including YFP, BFP, eGFP, mCherry, mOrange, mScarlet, and many more. All of our fluorescent proteins are optimized for expression in C. elegans via codon optimization and insertion of introns.

Use our fluorescent protein tags in combination with the Fluorescent Protein RNAi to reduce or eliminate protein expression.

Concerned that the addition of a fluorescent protein will inhibit protein function? We can use a 2A or SL2 sequence to separate the 2 components!

Things to consider when ordering your strain:

  • Fluorescent protein best suited for your experiments
  • Best terminus to tag

We are not limited to a fixed list of fluors. If you have a fluor that you would like to use, you can provide us the sequence and we will incorporate it into the design of your transgenic project. If you need your fluorescent transgenics lines to be imaged, we can readily help you with that too! If you are unsure, our genetic engineering experts will be glad to advise you. Contact us.

For immobilization of live animals to image yourself, learn more about NemaGel.

Service Details (price reflects academic pricing)

Service PackagePriceEst. Delivery Time
Full Build $4,815
8 -12 Weeks
Candidate Lines $3,434
5 - 7 Weeks
Custom Injection Mix $1,4954 - 5 Weeks
READY TO GET STARTED?

Ready to connect with us to learn more about working with our company or our technology?

Scroll to Top