C. elegans Endogenous Tagging Packages

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C. elegans Endogenous Tagging via CRISPR Gene Editing

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Tag your favorite gene at the native locus via CRISPR/Cas9 gene editing technology. Choose from a large selection of fluorescent proteins, immunotags and degron tags.

Custom tags and combinations (i.e. GFP::3xFLAG, etc.) are also available.

Process For Generating Strains

  • Transgenic design

    Transgenic Design

  • Molecular Assembly

    Molecular Assembly

  • Injection

    Injections

  • candidate screening

    Candidate Screening

  • C. elegans Confirmation

    Strain Confirmation

  1. Transgenic Design:

    Design of donor homology plasmid, guide sgRNAs and choice of a selection cassette for expression of your fluorescently tagged gene.
  2. Molecular Assembly:

    Confirm plasmids and construct high-quality components for genome editing.
  3. C. elegans Injections:

    Using a plasmid-based DNA injection mix, our skilled injectionists perform microinjections with high survival and transgenesis rates.
  4. Candidate Screening:

    Integration candidates are identified with the correct phenotype for the selection cassette. Confirm integration at the genomic level via PCR screening.
  5. Strain Confirmation:

    PCR testing to confirm that the lines have the desired construct integration and sequencing to confirm that the integration occurred without error.

C. elegans Endogenous Tagging Service Offerings

Degron Tag

Tag your protein of interest for controlled degradation.

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Fluorescent Tag

Visualize your protein via addition of a fluorescent protein tag at the native locus.

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Immunotag

Use your protein for biochemical studies by adding an immunotag at the native locus.

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Package Details (price reflects academic pricing)

Tagging ServiceFull Build Candidate Lines Custom Injection Mix
PriceDeliveryPriceDeliveryPriceDelivery
Degron tag$4,5607 - 8 Weeks$3,0396 - 7 Weeks$1,2955 - 6 Weeks
Fluorescent tag$4,8158 - 12 Weeks$3,4345 - 7 Weeks$1,4954 - 5 Weeks
Immunotag$4,3604 - 8 Weeks$2,8393 - 6 Weeks$1,0953 - 4 Weeks

Endogenous Tagging Publications

  • Diet-responsive Transcriptional Regulation of Insulin in a Single Neuron Controls Systemic Metabolism

    Ava Handley, Qiuli Wu, Tessa Sherry, Roger Pocock. bioRxiv. August 31, 2019.

  • The myosin light-chain kinase MLCK-1 relocalizes during Caenorhabditis elegans ovulation to promote actomyosin bundle assembly and drive contraction

    Charlotte A. Kelley, Alison C. E. Wirshing, Ronen Zaidel-Bar, and Erin J. Cram. MBoC. August 08, 2018: 1917-2035

  • The exocyst complex and Rab5 are required for abscission by localizing ESCRT III subunits to the cytokinetic bridge

    Harsh Kumar, Kumari Pushpa, Amrita Kumari, Kuldeep Verma, Rajaiah Pergu, Sivaram V. S. Mylavarapu. J. of Cell Science. 17 July 2019

  • Syncytial germline architecture is actively maintained by contraction of an internal actomyosin corset

    Priti A, Ong HT, Toyama Y, Padmanabhan A, Dasgupta S, Krajnc M, Zaidel-Bar R. Nat Commun. 2018 Nov 8;9(1):4694.

  • Disruption of the Caenorhabditis elegans Integrator complex triggers a non-conventional transcriptional mechanism beyond snRNA genes

    Gómez-Orte E, Sáenz-Narciso B, Zheleva A, Ezcurra B, de Toro M, López R, Gastaca I, Nilsen H, Sacristán MP, Schnabel R, Cabello J. PLoS Genetics. 2019 Feb 26;15(2):e1007981.

  • The conserved LEM-3/Ankle1 nuclease is involved in the combinatorial regulation of meiotic recombination repair and chromosome segregation in Caenorhabditis elegans

    Hong Y, Velkova M, Silva N, Jagut M, Scheidt V, Labib K, Jantsch V, Gartner A. PLoS Genetics. 2018 June 7;14(6):e1007453.

  • The exocyst complex and Rab5 are required for abscission by localizing ESCRT III subunits to the cytokinetic bridge

    Kumar H, Pushpa K, Kumari A, Verma K, Pergu R, Mylavarapu SVS. J. Cell Science. 2019 July 17;132(14).

  • LEM-3 is a midbody-tethered DNA nuclease that resolves chromatin bridges during late mitosis

    Hong Y, Sonneville R, Wang B, Scheidt V, Meier B, Woglar A, Demetriou S, Labib K, Jantsch V, Gartner A. Nat Commun. 2018 Feb 20;9(1):728.

  • The myosin light chain kinase, MLCK-1 relocalizes during C. elegans ovulation to promote actomyosin bundle assembly and drive contraction

    Kelley CA, Wirshing ACE, Zaidel-Bar R, Cram EJ. Mol Biol Cell. 2018 Aug 8;29(16):1975-1991.

  • CUL-2LRR-1 and UBXN-3 drive replisome disassembly during DNA replication termination and mitosis

    Sonneville, R; Moreno, SP; Knebel, A; Johnson, C; Hastie, CJ; Gartner, A; Gambus, A; Labib, K. Nat Cell Biol. 2017 May;19(5):468-479.

  • Olfactory stem cells reveal MOCOS as a new player in autism spectrum disorders

    Féron, F; Gepner, B; Lacassagne, E; Stephan, D; Mesnage, B; Blanchard, MP; Boulanger, N; Tardif, C; Devèze, A; Rousseau, S; Suzuki, K; Izpisua Belmonte, JC; Khrestchatisky, M; Nivet, E; Erard-Garcia, M. Mol Psychiatry. 2016 Sep;21(9):1215-24.

  • Ribonuclease-Mediated Control of Body Fat

    Habacher, C; Guo, Y; Venz, R; Kumari, P; Neagu, A; Gaidatzis, D; Harvald, EB; Færgeman, NJ; Gut, H; Ciosk, R. Dev Cell. 2016 Nov 7;39(3):359-369.

  • Replication-Independent Histone Variant H3.3 Controls Animal Lifespan through the Regulation of Pro-longevity Transcriptional Programs

    Piazzesi, A; Papić, D; Bertan, F; Salomoni, P; Nicotera, P; Bano, D. Cell Rep. 2016 Oct 18;17(4):987-996.

  • The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development

    Cottee, PA; Cole, T; Schultz, J; Hoang, HD; Vibbert, J; Han, SM; Miller, MA. Development. 2017 Jun 15;144(12):2187-2199.

  • Mechanism of biofilm-mediated stress resistance and lifespan extension in C. elegans

    Smolentseva, O; Gusarov, I; Gautier, L; Shamovsky, I; DeFrancesco, AS; Losick, R; Nudler, E. Sci Rep. 2017 Aug 2;7(1):7137.

  • Regulation of DJ-1 by Glutaredoxin 1 in Vivo: Implications for Parkinson’s Disease

    Johnson, WM; Golczak, M; Choe, K; Curran, PL; Miller, OG; Yao, C; Wang, W; Lin, J; Milkovic, NM; Ray, A; Ravindranath, V; Zhu, X; Wilson, MA; Wilson-Delfosse, AL; Chen, SG; Mieyal, JJ. Biochemistry. 2016 Aug 16;55(32):4519-32.

  • Caenorhabditis elegans BUB-3 and SAN-1/MAD3 Spindle Assembly Checkpoint Components Are Required for Genome Stability in Response to Treatment with Ionizing Radiation

    Bertolini, S;Wang, B;Meier, B;Hong, Y;Gartner, A. G3 (Bethesda). 2017 Dec 4;7(12):3875-3885.

  • A Bystander Mechanism Explains the Specific Phenotype of a Broadly Expressed Misfolded Protein

    Klabonski, L;Zha, J;Senthilkumar, L;Gidalevitz, T. PLoS Genet. 2016 Dec 7;12(12):e1006450.

  • A Differentiation Transcription Factor Establishes Muscle-Specific Proteostasis in Caenorhabditis elegans

    Bar-Lavan, Y; Shemesh, N; Dror, S; Ofir, R; Yeger-Lotem, E; Ben-Zvi, A. PLoS Genet. 2016 Dec 30;12(12):e1006531.

  • The nphp-2 and arl-13 genetic modules interact to regulate ciliogenesis and ciliary microtubule patterning in C. elegans

    Warburton-Pitt, SR; Silva, M; Nguyen, KC; Hall, DH; Barr, MM. PLoS Genet. 2014 Dec 11;10(12):e1004866.

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