C. elegans Endogenous Tagging via CRISPR Gene Editing
Tag your favorite gene at the native locus via CRISPR/Cas9 gene editing technology. Choose from a large selection of fluorescent proteins, immunotags and degron tags.
Custom tags and combinations (i.e. GFP::3xFLAG, etc.) are also available.
Process For Generating Strains
- Transgenic Design:
Design of donor homology plasmid, guide sgRNAs and choice of a selection cassette for expression of your fluorescently tagged gene.
- Molecular Assembly:
Confirm plasmids and construct high-quality components for genome editing.
- C. elegans Injections:
Using a plasmid-based DNA injection mix, our skilled injectionists perform microinjections with high survival and transgenesis rates.
- Candidate Screening:
Integration candidates are identified with the correct phenotype for the selection cassette. Confirm integration at the genomic level via PCR screening.
- Strain Confirmation:
PCR testing to confirm that the lines have the desired construct integration and sequencing to confirm that the integration occurred without error.
C. elegans Endogenous Tagging Service Offerings
Package Details (price reflects academic pricing)
Endogenous Tagging Publications
Disruption of the Caenorhabditis elegans Integrator complex triggers a non-conventional transcriptional mechanism beyond snRNA genes
Gómez-Orte E, Sáenz-Narciso B, Zheleva A, Ezcurra B, de Toro M, López R, Gastaca I, Nilsen H, Sacristán MP, Schnabel R, Cabello J. PLoS Genetics. 2019 Feb 26;15(2):e1007981.
Olfactory stem cells reveal MOCOS as a new player in autism spectrum disorders
Féron, F; Gepner, B; Lacassagne, E; Stephan, D; Mesnage, B; Blanchard, MP; Boulanger, N; Tardif, C; Devèze, A; Rousseau, S; Suzuki, K; Izpisua Belmonte, JC; Khrestchatisky, M; Nivet, E; Erard-Garcia, M. Mol Psychiatry. 2016 Sep;21(9):1215-24.
Regulation of DJ-1 by Glutaredoxin 1 in Vivo: Implications for Parkinson’s Disease
Johnson, WM; Golczak, M; Choe, K; Curran, PL; Miller, OG; Yao, C; Wang, W; Lin, J; Milkovic, NM; Ray, A; Ravindranath, V; Zhu, X; Wilson, MA; Wilson-Delfosse, AL; Chen, SG; Mieyal, JJ. Biochemistry. 2016 Aug 16;55(32):4519-32.