C. elegans Behavior & Phenotyping
Reveal developmental effects of mutations and compound treatment by quantifying brood sizes.
Mutations and compounds can affect animal viability and progeny production. Quantification of brood size can reveal developmental effects of mutations and compound treatment.
Single L4 C. elegans are incubated on a plate at 20℃ where they become adults and start laying eggs. The adult C. elegans are removed after 48 hours. Two days later the number of L3/L4 and adult worms are counted.
The number of progeny produced by each single animal is determined. Five biological replicates are tested.