C. elegans Behavior & Phenotyping
The balance between lipid storage and utilization is an organism-wide process involving behavior, metabolism and signaling.
Lipid storage diseases such as Niemann-Pick are commonly studied in C. elegans.
Animals are stained with RediStain Lipid Green, a lipophilic fluorophore that tracks lipids inside C. elegans. When using Lipid Green, native lipid droplet morphology is preserved and fat content per body volume is quantified in individual worms.
Images of lipid droplets and quantification of fluorescence for 10 worms/condition are collected.