C. elegans Behavior & Phenotyping
Defecation is an highly regulated ultradian rhythmic process. It can be used to assess defects in neuromuscular signaling.
Can be used to study diseases such as Epilepsy, Alzheimer disease and Parkinson’s disease.
Pick one young adult worm to a fresh NGM plate seeded with OP50. Wait 5 min (Worm will not feed or defecate for 1-2 min because of transferring.) and take a video for 10 min.
The dataset includes three biological replicates. We collect over 10 worm videos and analyze defecation interval cycle (pBoc-pBoc time) and % of expulsion in 10 min recording. Statistical results are analyzed by ANOVA test.