C. elegans Behavior & Phenotyping Capabilities

C. elegans Behavior & Phenotyping

Imaging

Observe, locate and quantify the expression of a fluorescence-tagged protein in a whole animal.

Left: Amphid sensory neurons are stained with Neuro Red–DiI staining.
Right: Confocal microscopy of LMN-1::GFP.

  • APPLICATIONS

    Obtain high quality images to highlight changes in morphology or fluorescence in each individual. Detect & visualize morphological defects of a genetically modified strain.

  • ASSAY DESCRIPTION

    High powered microscopy is used to capture brightfield and fluorescent images of live animals. Fluorescent proteins and fluorescent dyes can both be observed as appropriate.

  • DATA COLLECTED

    Images and quantification of fluorescence for 10 worms/condition will be collected.

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