C. elegans Behavior & Phenotyping
Reveal developmental effects of mutations and compound treatment by quantifying embryo viability.
Mutations and compounds can affect animal viability. Embryo viability can reveal developmental effects of mutations and compound treatment.
Adult C. elegans are allowed to lay eggs on a plate for 2 hours. A set number of embryos are moved to a new plate. After 24 hours of incubation at 20℃, the number of unhatched embryos is counted.
The percentage of embryo lethality is calculated from the unhatched embryos divided by the total number of embryos plated. Three replicates are tested.