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Expert C. elegans Gene Editing Services

Unlock your research to its full potential with our comprehensive CRISPR C. elegans gene editing services. As leading experts in transgenic services and CRISPR injection techniques, we specialize in designing, constructing, and delivering custom genome-edited C. elegans specimens, including CRISPR knock-in and knockout cell line services optimized to meet your precise specifications.

Why Work With Us

InVivo Biosystems has successfully delivered over 3,000 C. elegans transgenic lines to researchers who rely on our genome-edited strains to generate data and publish results .Our work significantly contributes to advancements in regenerative medicine and the development of cell therapy applications  aiding affordable therapeutics in rare diseases.

As an officially licensed CRISPR solutions provider, we have developed several unique internal processes to ensure the quality of the strains and to improve editing efficiency, with a particular emphasis on the successful gene editing platform.

 

 

Types Of Genome-Editing Services

Our collaborative project design process is tailored to meet your specific research needs, whether you’re looking for CRISPR knock-in cell lines, knockout cell line service, or need assistance with creating an extrachromosomal array in C. elegans. Our comprehensive gene editing services are designed to facilitate your research in drug discovery and the generation of disease models.

Introduce a small number of nucleotide changes at a target site. Often used to study a disease-causing mutation, understand gene function, or more precisely model human diseases.
Disrupt or delete integral domains in the coding sequence of a gene. Commonly used to study gene function. We can delete the entire coding sequence of a gene or any targeted genomic region.
Disrupt or delete integral domains in the coding sequence of a gene. Commonly used to study gene function. We can delete the entire coding sequence of a gene or any targeted genomic region.
The simplest and most frequently used transgenesis procedure, enables desired construct to be expressed without being integrated into the genome.

C. elegans Transgenic Service Packages

We offer a variety of C. elegans services packages to fit the needs and budget. Choose from our diverse service packages, including CRISPR injection for precise gene editing, and our specialized services for creating extrachromosomal array C. elegans lines. Our transgenic services provide the support you need to achieve your research goals, focusing on gene knock and gene target strategies for precise target sequence identification.

 

Build Type
Full Build
We do it all and deliver you a verified line.
Candidate Lines
We get you the lines that pass the selection marker screen.
Custom Injection Mix
We get you the components that allow you to inject and find the transgenics you need.
Inject Express
You provide the mix, we inject and send you back the F1 broods.
Transgenic Design
Injections
Candidate Screening
Candidate Screening

Other Gene Editing Services We Provide

Why Choose InVivo Biosystems for Your Custom Genome-Edited C. elegans Services

  • Experience and Expertise: With over 3,000 successful C. elegans transgenic lines delivered, our team has the experience and expertise to handle projects of any complexity.
  • State-of-the-Art Techniques: As an officially licensed CRISPR solutions provider, we employ the latest genome editing technologies to achieve the highest efficiency and success rates.
  • Collaborative Approach: We work closely with our clients to design and deliver custom C. elegans strains tailored to their research needs, ensuring transparency and satisfaction throughout the process.
  • Quality Assurance: Our stringent internal quality control processes guarantee the highest quality strains, enabling you to generate reliable data and publish meaningful results.

Workflow for Generating C. elegans Strains

InVivo Biosystems provides a streamlined process for C. elegans gene editing services, employing state-of-the-art genome editing tools and gene editing experiments methodologies.

Transgenic Design

We collaborate with you to understand your gene expression expectations. This allows us to find the most... optimal promoter, coding, and terminator sequences to maximize the chance of successful strain generation. We listen to your needs and adapt the project accordingly. See more

01

Molecular Assembly

Depending on the project approach, we often turn to de novo sequence generation through our suppliers... Alternatively, we can deploy a variety of gene assembly approaches to get the exact sequence needed for the appropriate tissue-specific expression. Either way, we efficiently generate the sequence components needed for creation of high- efficiency injection mix components. See more

02

C. elegans Injections

With thousands of lines delivered, our team of injectionists have the necessary skills. They can ... obtain precision delivery of transgenesis reagents to the nematode gonad. Often, only a few animals need to be injected in order to generate multiple biological replicates. See more

03

Strain Confirmation

Often our process has a selection marker that helps us identify candidates, but we don't stop there. For our ... genomic integrants, we look at each transgenesis junction by PCR. Once we confirm the ends of the gene insertion have proceeded as planned, clients can opinion for full sequencing of the entire transgene sequence so that a sequence verified strain is delivered. See more

05

Candidate Screening

We perform a sufficient number of injections that typically 3 or more biological replicates ... are generated. Occasionally the numbers of animals transformed is below this number in a set of injected animals. When this is detected, we quickly redo another set of injections to ensure that you received a good chance at multiple biological replicate strains. See more

04

Why Work With Us

InVivo Biosystems has successfully delivered over 3,000 C. elegans transgenic lines to researchers who rely on our genome-edited strains to generate data and publish results and seek affordable therapeutics in rare diseases.

As an officially licensed CRISPR solutions provider, we have developed several unique internal processes to ensure the quality of the strains and to improve editing efficiency.

Genome Editing Service Cycle

Before You Start Your Project

Consultation with our genome engineering design experts to build the exact custom transgenic you need for your research.

During Your Project

Bi-weekly updates about the progress made on your project from our team of genome engineers. Key milestones will be noted.

After Your Project

Receive a validation report that describes the details of your genome edit and shows the evidence that the desired edit was made.

Billing Practices

Strain Designation

At the request of WormBase, genetic variation generated and recovered by InVivo Biosystems should retain the “knu # “ allele designation provided. This includes variations involving CRISPR Knock-In and DNA insertion methods.

Allele names can be ascertained from either product shipping documents or by emailing InVivo Biosystems . For example: The strain gene-1(knu123[GFP::gene-1]) was generated by InVivo Biosystems.

Frequently Asked Questions

InVivo Biosystems has successfully delivered 3,000 C. elegans transgenic lines to researchers who rely on our genome-edited strains to generate data and publish results. As an expert in C. elegans genome editing, we have developed many unique internal processes to ensure the quality of the strains, including plasmid validation, injection protocol, and to improve our editing efficiency. Choices of genome editing methods are CRISPR, MosSCI and Extrachromosomal Array.

C. elegans has become a key model for understanding the molecular mechanisms involved in a growing number of human diseases. Having a short generation time along with invariant developmental lineage, C. elegans has proved to be extremely beneficial for understanding basic cell biology, neurobiology and aging. Another unique advantage of C. elegans over other multicellular model organisms is that individuals can be frozen indefinitely and revived to obtain an F2 generation within a week, thus eliminating the cost of maintaining colonies of mutants

Yes, our strain and allele designation have been confirmed by the WormBase, and any genetic variation generated and recovered by InVivo Biosystems should retain the designated “knu # “ allele name. Allele names can be ascertained from either product shipping documents or by emailing InVivo Biosystems . For example: The strain gene-1 (knu123[GFP::gene-1]) was generated by InVivo Biosystems 

We enable you to tag your favorite gene at the native locus via CRISPR/Cas9 gene editing technology choosing from a large selection of fluorescent proteins, immunotags and degron tags. Custom tags and combinations (i.e. GFP::3xFLAG, etc.) are also available.

InVivo Biosystems has the capability to make specific DNA alterations including knockout, point mutation, precise deletion, and floxed allele with high precision which enables you to study gene function in exciting new ways.

We offer MosSCI and Extrachromosomal array with varying cost and delivery time. Please view our package details for further information.

To quantify the expression of a transgene in C. elegans, techniques like Quantitative Polymerase Chain Reaction (qPCR) and Western Blotting are effective. qPCR is ideal for measuring mRNA levels of the transgene, providing precise quantitative data. For protein expression, Western Blotting is used to detect and quantify the transgenic protein using specific antibodies. These methods provide accurate and reliable quantification of transgene expression in genetic research.

 

Yes, we offer custom injection services where you provide the gene editing idea in your strain receive professionally prepared injection mix containing all of the required molecular components to achieve your desired custom genetic edit. You will, however, require access to microinjection rig, technical skills for proper injection, and the ability to screen and select final desired strain(s).

We offer a 50/50 service payment plan for Full Build and Candidate Lines projects. The first 50% is required for project start and is non-refundable. The second 50% is paid at end of the project and is only billable if a line is delivered.

Prices stated are exclusive of all taxes, fees, licenses, duties or levies and, unless otherwise stated in the quotation, transportation charges, freight and insurance.

InVivo Biosystems quotations are valid for thirty (30) days unless otherwise stated in the quotation. Please report within the mentioned duration.

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