Insertion of two loxP sites to produce a knockout (KO) of an embryonic lethal gene

A client wanted a knockout (KO) of an embryonic lethal gene. We could not make this line using our standard methods. Instead, we inserted two loxP sites. One in the first intron of the gene and the second in the 3’utr. After we confirmed this line by PCR and sequencing, we injected this line with …

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Analyzing Small C. elegans Populations with the wMicroTracker

Most experiments are based on population analysis. However, some researchers are interested in the study of a single individual. In wMicrotracker it is possible to perform experiments with as few as 1 worm. Here are some tips for doing this type of assay. To increase data accuracy, it is important to use an appropriate number of …

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