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Trisha Brock

Insertion of two loxP sites to produce a knockout (KO) of an embryonic lethal gene

A client wanted a knockout (KO) of an embryonic lethal gene. We could not make this line using our standard methods. Instead, we inserted two loxP sites. One in the first intron of the gene and the second in the 3’utr. After we confirmed this line by PCR and sequencing, we injected this line with …

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