Trisha Brock InVivo Biosystems

We Used CRISPR to Make 15 Point Mutations in 5 Genes. Here’s What We Learned.

C. elegans, Model Organism / By Trisha Brock and Kathryn McCormick / June 18, 2020 June 29, 2020

You may or may not know that at InVivo Biosystems, we conduct a lot of grant funded research as well as offering custom models and assays to our clients. Much of our grant activity in the past few years has centered on creating and characterizing disease-associated variants and point mutations. We’ve created collaborations and partnerships …

We Used CRISPR to Make 15 Point Mutations in 5 Genes. Here’s What We Learned. Read More »

zebrafish crispr knock in protocol_workflow

11 Steps to a Successful CRISPR Knock-in

Model Organism, Zebrafish / By Trisha Brock / April 30, 2020 May 12, 2020

Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in zebrafish so we want to share with you what we believe to be the 11 steps necessary to have a successful knock-in project.

Insertion of two loxP sites to produce a knockout (KO) of an embryonic lethal gene

Model Organism / By Trisha Brock / October 3, 2018 January 10, 2019

A client wanted a knockout (KO) of an embryonic lethal gene. We could not make this line using our standard methods. Instead, we inserted two loxP sites. One in the first intron of the gene and the second in the 3’utr. After we confirmed this line by PCR and sequencing, we injected this line with …

Insertion of two loxP sites to produce a knockout (KO) of an embryonic lethal gene Read More »