The development of CRISPR-Cas9 editing has allowed researchers to quickly create precise gene knockouts (KO), but while CRISPR-Cas9 KOs have been highly publicized, are the different methods understood? In this article we will discuss the two available methods for creating knockout models using the CRISPR-Cas9 technology: their advantages and limitations, and how they are being used in research.
Learn more about the DrugAge Database which highlights the high translational value of C. elegans as a model organism, and shows how valuable they can be as a tool for identifying novel drugs and drug targets in longevity research.
Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.
We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants were selected from the ClinVar database, literature, the Gnomad database, clinical researchers, and the STXBP1 foundation.
You may or may not know that at InVivo Biosystems, we conduct a lot of grant funded research as well as offering custom models and assays to our clients. Much of our grant activity in the past few years has centered on creating and characterizing disease-associated variants and point mutations. We’ve created collaborations and partnerships …
We Used CRISPR to Make 15 Point Mutations in 5 Genes. Here’s What We Learned. Read More »
Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in zebrafish so we want to share with you what we believe to be the 11 steps necessary to have a successful knock-in project.
A client wanted a knockout (KO) of an embryonic lethal gene. We could not make this line using our standard methods. Instead, we inserted two loxP sites. One in the first intron of the gene and the second in the 3’utr. After we confirmed this line by PCR and sequencing, we injected this line with …
Insertion of two loxP sites to produce a knockout (KO) of an embryonic lethal gene Read More »
