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Ben Jussila

Zebrafish Acute Toxicity Study Demonstrates Concordance with Rat Fetal Embryotoxicity

Summary: As the zebrafish is increasingly adopted to test drug toxicity, it is critical to systematically assess the parallels between zebrafish, humans and other model systems. In this article, we highlight a recent paper that compares toxicity results obtained in zebrafish and rats. The paper concludes that zebrafish are “valuable for predicting mammalian toxic exposure …

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6 things that can go wrong while making your Zebrafish CRISPR Knock-In

With the advent of targeted nucleases like zinc-finger nucleases (ZFNs), TALE nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas), zebrafish researchers were able to begin interrogating gene function and regions of interest with a precision that was previously out of reach. With these innovations, researchers could not only disrupt …

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Conditional mutagenesis by loxP insertion in zebrafish – outfoxing the unfloxable

CRISPR/Cas9-mediated integration of loxP sequences for conditional mutagenesis in zebrafish has recently made its way into the toolkit of zebrafish researchers. In particular, CRISPR/Cas9 mutagenesis coupled with ssODN-mediated repair has simplified experimental design and provided a robust framework for conditional mutagenesis, as readily demonstrated by several groups in the zebrafish community to date….

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CRISPR/Cas9 off target effects: Hyperactive cutting or media hype?

There has been a lot of media coverage in recent months surrounding CRISPR/Cas9 and the potentially life changing impact the gene-editing technology could have. More recently though, this coverage has focused on the potential for serious consequences in the form of off-target effects. The fear with off-target effects is that CRISPR/Cas9 editing is not as …

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An Abnormal Absence of Antibodies: Filling the Gaps with Gene Editing Tools in Zebrafish

The zebrafish is an increasingly popular laboratory organism with many experimental advantages. The animal model has been used by biologists to discover the detailed processes that underlie vertebrate development and disease, and study vertebrate haematopoiesis and immune cell interactions1. The zebrafish embryo is also a popular vertebrate model for neurobiology2. Zebrafish research, however, is limited …

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